Expression of Recombinant E1 Glycoprotein of Chikungunya Virus in Baculovirus Expression System
Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, the envelope E1 gene of CHIKV was cloned and expressed in a baculovirus system. The recombinant E1 protein with N-term 6-His residues protein was successfully expressed and purified as confirmed by SDS-PAG...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hibiscus Publisher
2015
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/9288/ http://ir.unimas.my/id/eprint/9288/1/Expression%20of%20Recombinant%20E1%20Glycoprotein%20of%20Chikungunya%20Virus%20in%20Baculovirus%20Expression%20System%20%28abstract0.pdf |
| Summary: | Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, the
envelope E1 gene of CHIKV was cloned and expressed in a baculovirus system. The
recombinant E1 protein with N-term 6-His residues protein was successfully expressed and
purified as confirmed by SDS-PAGE and western blot analysis. The seroreactivity of the
recombinant protein was evaluated in immunoassay for anti-CHIKV IgM and IgG antibodies.
The recombinant antigen showed 69% sensitivity and 100% specificity for anti-CHIKV IgG by
dot blot assay. Detection of anti-CHIKV IgM by dot assay showed 79% sensitivity and 100%
specificity. No cross reactivity of the antigen was observed with anti-dengue virus serum
samples. The results strongly support that the recombinant E1 protein has potential to be used as
diagnostic antigen. The used of the antigen in a dot blot assay gives an advantage for laboratory
detection without the need of any specialised equipment. |
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