The Effect of Hydrocotyle sibthorpioides Lam. Extracts on in Vitro Dengue Replication
Objective. To investigate the potential effect of Hydrocotyle sibthorpioides Lam. (H. sibthorpioides) extracts against in vitro dengue viral replication. Methods. The cytotoxicity of H. sibthorpioides was evaluated using a cell viability assay. Cells were pre- and posttreated with water and methanol...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Publishing Corporation
2015
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/9283/ http://ir.unimas.my/id/eprint/9283/1/NO%2017%20The%20Effect%20of%20Hydrocotyle%20sibthorpioides%20Lam.%20Extracts%20on%20In%20Vitro%20Dengue%20Replication%28abstract%29.pdf |
| _version_ | 1848836529445666816 |
|---|---|
| author | Fitrien, Husin Yean, Yean Chan Siew Hua, Gan Siti Amrah, Sulaiman Rafidah Hanim, Shueb |
| author_facet | Fitrien, Husin Yean, Yean Chan Siew Hua, Gan Siti Amrah, Sulaiman Rafidah Hanim, Shueb |
| author_sort | Fitrien, Husin |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Objective. To investigate the potential effect of Hydrocotyle sibthorpioides Lam. (H. sibthorpioides) extracts against in vitro dengue viral replication. Methods. The cytotoxicity of H. sibthorpioides was evaluated using a cell viability assay. Cells were pre- and posttreated with water and methanol extracts of H. sibthorpioides, and the viral inhibitory effect was investigated by observing the morphological changes, which were further confirmed by plaque assay. Results. The methanolic extract cytotoxicity was higher in Vero and C6/36 cells than the cytotoxicity of the water extract. Preincubation of the cells with H. sibthorpioides extract showed nonexistent to mild prophylactic effects. The posttreatment of Vero cells with H. sibthorpioides methanolic extract presented higher antidengue activities when compared with the water extract. Surprisingly, posttreatment of C6/36 cells resulted in an enhancement of viral replication. Conclusion. H. sibthorpioides had variable effects on dengue viral replication, depending on the treatment, cell lines, and solvent types. This study provides important novel insights on the phytomedicinal properties of H. sibthorpioides extracts on dengue virus. |
| first_indexed | 2025-11-15T06:25:13Z |
| format | Article |
| id | unimas-9283 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:25:13Z |
| publishDate | 2015 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-92832015-11-03T02:07:52Z http://ir.unimas.my/id/eprint/9283/ The Effect of Hydrocotyle sibthorpioides Lam. Extracts on in Vitro Dengue Replication Fitrien, Husin Yean, Yean Chan Siew Hua, Gan Siti Amrah, Sulaiman Rafidah Hanim, Shueb RZ Other systems of medicine Objective. To investigate the potential effect of Hydrocotyle sibthorpioides Lam. (H. sibthorpioides) extracts against in vitro dengue viral replication. Methods. The cytotoxicity of H. sibthorpioides was evaluated using a cell viability assay. Cells were pre- and posttreated with water and methanol extracts of H. sibthorpioides, and the viral inhibitory effect was investigated by observing the morphological changes, which were further confirmed by plaque assay. Results. The methanolic extract cytotoxicity was higher in Vero and C6/36 cells than the cytotoxicity of the water extract. Preincubation of the cells with H. sibthorpioides extract showed nonexistent to mild prophylactic effects. The posttreatment of Vero cells with H. sibthorpioides methanolic extract presented higher antidengue activities when compared with the water extract. Surprisingly, posttreatment of C6/36 cells resulted in an enhancement of viral replication. Conclusion. H. sibthorpioides had variable effects on dengue viral replication, depending on the treatment, cell lines, and solvent types. This study provides important novel insights on the phytomedicinal properties of H. sibthorpioides extracts on dengue virus. Hindawi Publishing Corporation 2015 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/9283/1/NO%2017%20The%20Effect%20of%20Hydrocotyle%20sibthorpioides%20Lam.%20Extracts%20on%20In%20Vitro%20Dengue%20Replication%28abstract%29.pdf Fitrien, Husin and Yean, Yean Chan and Siew Hua, Gan and Siti Amrah, Sulaiman and Rafidah Hanim, Shueb (2015) The Effect of Hydrocotyle sibthorpioides Lam. Extracts on in Vitro Dengue Replication. Evidence-Based Complementary and Alternative Medicine, 2015. http://www.hindawi.com/journals/ecam/2015/596109/ http://dx.doi.org/10.1155/2015/596109 |
| spellingShingle | RZ Other systems of medicine Fitrien, Husin Yean, Yean Chan Siew Hua, Gan Siti Amrah, Sulaiman Rafidah Hanim, Shueb The Effect of Hydrocotyle sibthorpioides Lam. Extracts on in Vitro Dengue Replication |
| title | The Effect of Hydrocotyle sibthorpioides Lam. Extracts on in Vitro Dengue Replication |
| title_full | The Effect of Hydrocotyle sibthorpioides Lam. Extracts on in Vitro Dengue Replication |
| title_fullStr | The Effect of Hydrocotyle sibthorpioides Lam. Extracts on in Vitro Dengue Replication |
| title_full_unstemmed | The Effect of Hydrocotyle sibthorpioides Lam. Extracts on in Vitro Dengue Replication |
| title_short | The Effect of Hydrocotyle sibthorpioides Lam. Extracts on in Vitro Dengue Replication |
| title_sort | effect of hydrocotyle sibthorpioides lam. extracts on in vitro dengue replication |
| topic | RZ Other systems of medicine |
| url | http://ir.unimas.my/id/eprint/9283/ http://ir.unimas.my/id/eprint/9283/ http://ir.unimas.my/id/eprint/9283/ http://ir.unimas.my/id/eprint/9283/1/NO%2017%20The%20Effect%20of%20Hydrocotyle%20sibthorpioides%20Lam.%20Extracts%20on%20In%20Vitro%20Dengue%20Replication%28abstract%29.pdf |