Optimization of metabolite extraction from sago leaves tissue

Metabolic profilling have been widely studies in plants especially for highly commercial product. Currently, the study of metabolites in Metroxylon sagu becomes significantly interesting as it is one of the starch-rich sources for many potential industries. Extraction is the most critical step in...

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Main Author: Farhana, Binti Aktar
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2013
Subjects:
Online Access:http://ir.unimas.my/id/eprint/8755/
http://ir.unimas.my/id/eprint/8755/8/Farhana%20Aktar%20ft.pdf
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author Farhana, Binti Aktar
author_facet Farhana, Binti Aktar
author_sort Farhana, Binti Aktar
building UNIMAS Institutional Repository
collection Online Access
description Metabolic profilling have been widely studies in plants especially for highly commercial product. Currently, the study of metabolites in Metroxylon sagu becomes significantly interesting as it is one of the starch-rich sources for many potential industries. Extraction is the most critical step in metabolites profiling. This study was conducted to optimize several parameters in the extraction method of metabolites of sago leaves. The parameters were temperature for extraction, extraction period and extraction times which were tested using solvent extraction method. Methanol chloroform co-solvent was used in extraction with ratio 2 : 1.The data was measured in quantity by weighing the metabolites extracted. From factorial analysis, the result showed there was significant correlation between temperature, extraction period and extraction time towards metabolites extracted in leaves tissue of M. sagu. Optimal condition towards metabolites extraction was at temperature 30oC for 2 hours at first batch extraction.
first_indexed 2025-11-15T06:23:46Z
format Final Year Project Report / IMRAD
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institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:23:46Z
publishDate 2013
publisher Universiti Malaysia Sarawak, (UNIMAS)
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spelling unimas-87552023-08-28T07:50:36Z http://ir.unimas.my/id/eprint/8755/ Optimization of metabolite extraction from sago leaves tissue Farhana, Binti Aktar SB Plant culture Metabolic profilling have been widely studies in plants especially for highly commercial product. Currently, the study of metabolites in Metroxylon sagu becomes significantly interesting as it is one of the starch-rich sources for many potential industries. Extraction is the most critical step in metabolites profiling. This study was conducted to optimize several parameters in the extraction method of metabolites of sago leaves. The parameters were temperature for extraction, extraction period and extraction times which were tested using solvent extraction method. Methanol chloroform co-solvent was used in extraction with ratio 2 : 1.The data was measured in quantity by weighing the metabolites extracted. From factorial analysis, the result showed there was significant correlation between temperature, extraction period and extraction time towards metabolites extracted in leaves tissue of M. sagu. Optimal condition towards metabolites extraction was at temperature 30oC for 2 hours at first batch extraction. Universiti Malaysia Sarawak, (UNIMAS) 2013 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/8755/8/Farhana%20Aktar%20ft.pdf Farhana, Binti Aktar (2013) Optimization of metabolite extraction from sago leaves tissue. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle SB Plant culture
Farhana, Binti Aktar
Optimization of metabolite extraction from sago leaves tissue
title Optimization of metabolite extraction from sago leaves tissue
title_full Optimization of metabolite extraction from sago leaves tissue
title_fullStr Optimization of metabolite extraction from sago leaves tissue
title_full_unstemmed Optimization of metabolite extraction from sago leaves tissue
title_short Optimization of metabolite extraction from sago leaves tissue
title_sort optimization of metabolite extraction from sago leaves tissue
topic SB Plant culture
url http://ir.unimas.my/id/eprint/8755/
http://ir.unimas.my/id/eprint/8755/8/Farhana%20Aktar%20ft.pdf