Characterization and expression studies of gigyf2 gene in danio rerio

Grb 10 Interacting GYF protein 2 (GIGYF2) is one of the novel protein that interacts with growth factor receptor bound protein 10 (Grb10), an adapter protein that binds activated insulin-like growth factor I (IGF-I) and insulin receptors. The interaction suggests that the gene to be potentially invo...

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Bibliographic Details
Main Author: Lim, Shing Yiing
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2010
Subjects:
Online Access:http://ir.unimas.my/id/eprint/8720/
http://ir.unimas.my/id/eprint/8720/1/Lim%20Shing%20Yiing%20ft.pdf
Description
Summary:Grb 10 Interacting GYF protein 2 (GIGYF2) is one of the novel protein that interacts with growth factor receptor bound protein 10 (Grb10), an adapter protein that binds activated insulin-like growth factor I (IGF-I) and insulin receptors. The interaction suggests that the gene to be potentially involved in insulin and IGF signaling. The GIGYF2 gene is located within a region linked to familial Parkinson’s disease (PARK11 locus). Therefore, GIGYF2 represents a good candidate gene for PD. The expression of GIGYF2 gene was determined through RT-PCR of mRNA from ten isolated Danio rerio (zebrafish) organs, which include eye, gill, brain, heart, liver, intestine, stomach, skin, muscle and bone. The expression patterns of the GIGYF2 gene were compared between Danio rerio, human and mouse. Comparison showed that GIGYF2 gene was mainly expressed in insulin-targeted organs in Danio rerio and mammals.