Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques
Duabanga moluccana (or locally known as sawih) is an indigenous fast growing tropical tree species that confers various advantages for the timber industry and for planted forests development. In this paper, we isolated and characterized 8 polymorphic microsatellite markers from the D. moluccana geno...
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| Format: | Article |
| Language: | English |
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Springer India
2015
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| Online Access: | http://ir.unimas.my/id/eprint/8418/ http://ir.unimas.my/id/eprint/8418/1/Development.pdf |
| _version_ | 1848836372613300224 |
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| author | Liew, Kit-Siong Abdullah, Julaihi Pang Shek, Ling Ho Wei, Seng |
| author_facet | Liew, Kit-Siong Abdullah, Julaihi Pang Shek, Ling Ho Wei, Seng |
| author_sort | Liew, Kit-Siong |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Duabanga moluccana (or locally known as sawih) is an indigenous fast growing tropical tree species that confers various advantages for the timber industry and for planted forests development. In this paper, we isolated and characterized 8 polymorphic microsatellite markers from the D. moluccana genome using ISSR-suppression PCR techniques. The number of alleles and PIC values ranged from 3 to 8 alleles per locus and from 0.488 to 0.792, respectively. Three microsatellite loci were deviated from Hardy-Weinberg equilibrium (P<0.05). The transferability rate ranged from 24 to 100 % among the three indigenous tree species tested. This indicates that the newly developed microsatellite markers would be useful tools for population genetic studies on D. moluccana and other indigenous tree species. |
| first_indexed | 2025-11-15T06:22:43Z |
| format | Article |
| id | unimas-8418 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:22:43Z |
| publishDate | 2015 |
| publisher | Springer India |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-84182022-01-28T00:32:23Z http://ir.unimas.my/id/eprint/8418/ Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques Liew, Kit-Siong Abdullah, Julaihi Pang Shek, Ling Ho Wei, Seng SB Plant culture Duabanga moluccana (or locally known as sawih) is an indigenous fast growing tropical tree species that confers various advantages for the timber industry and for planted forests development. In this paper, we isolated and characterized 8 polymorphic microsatellite markers from the D. moluccana genome using ISSR-suppression PCR techniques. The number of alleles and PIC values ranged from 3 to 8 alleles per locus and from 0.488 to 0.792, respectively. Three microsatellite loci were deviated from Hardy-Weinberg equilibrium (P<0.05). The transferability rate ranged from 24 to 100 % among the three indigenous tree species tested. This indicates that the newly developed microsatellite markers would be useful tools for population genetic studies on D. moluccana and other indigenous tree species. Springer India 2015 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/8418/1/Development.pdf Liew, Kit-Siong and Abdullah, Julaihi and Pang Shek, Ling and Ho Wei, Seng (2015) Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques. Physiology and Molecular Biology of Plants, 21 (1). pp. 163-165. ISSN 0974-0430 http://www.researchgate.net/publication/272075880_Development_and_characterization_of_microsatellite_markers_in_sawih_tree_(Duabanga_moluccana_Blume)_using_ISSR-suppression_PCR_techniques doi: 10.1007/s12298-014-0262-2 |
| spellingShingle | SB Plant culture Liew, Kit-Siong Abdullah, Julaihi Pang Shek, Ling Ho Wei, Seng Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques |
| title | Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques |
| title_full | Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques |
| title_fullStr | Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques |
| title_full_unstemmed | Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques |
| title_short | Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques |
| title_sort | development and characterization of microsatellite markers in sawih tree (duabanga moluccana blume) using issr-suppression pcr techniques |
| topic | SB Plant culture |
| url | http://ir.unimas.my/id/eprint/8418/ http://ir.unimas.my/id/eprint/8418/ http://ir.unimas.my/id/eprint/8418/ http://ir.unimas.my/id/eprint/8418/1/Development.pdf |