Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus
Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, a short 24-kDa N-terminal of E2 glycoprotein of chikungunya virus was cloned and expressed in E.coli expression system. The E2 recombinant proteinwas expressed as a fusion protein to 6-Histidine for ease of p...
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| Format: | Article |
| Language: | English |
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Trade Science Inc. - INDIA
2015
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| Online Access: | http://ir.unimas.my/id/eprint/8406/ http://ir.unimas.my/id/eprint/8406/1/Expression.pdf |
| _version_ | 1848836370746834944 |
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| author | Magdline Sia, Henry Sum Anna, Andrew |
| author_facet | Magdline Sia, Henry Sum Anna, Andrew |
| author_sort | Magdline Sia, Henry Sum |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, a short 24-kDa N-terminal of E2 glycoprotein of chikungunya virus was cloned and expressed in E.coli expression system. The E2 recombinant proteinwas expressed as a fusion protein to 6-Histidine for ease of purification. The expression of the 24-kDa recombinant protein was detected by SDS-PAGE and the protein reactivity was evaluated by western blot analysis. The immunogenicity of the 24 kDa proteinwas further tested against human positive and negative sera for chikungunya and dengue. The results showed that the recombinant antigen was able to detect CHIKV positive sera and no cross reactivity was observed with dengue virus positive serum. |
| first_indexed | 2025-11-15T06:22:41Z |
| format | Article |
| id | unimas-8406 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:22:41Z |
| publishDate | 2015 |
| publisher | Trade Science Inc. - INDIA |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-84062022-01-28T01:39:31Z http://ir.unimas.my/id/eprint/8406/ Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus Magdline Sia, Henry Sum Anna, Andrew R Medicine (General) Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, a short 24-kDa N-terminal of E2 glycoprotein of chikungunya virus was cloned and expressed in E.coli expression system. The E2 recombinant proteinwas expressed as a fusion protein to 6-Histidine for ease of purification. The expression of the 24-kDa recombinant protein was detected by SDS-PAGE and the protein reactivity was evaluated by western blot analysis. The immunogenicity of the 24 kDa proteinwas further tested against human positive and negative sera for chikungunya and dengue. The results showed that the recombinant antigen was able to detect CHIKV positive sera and no cross reactivity was observed with dengue virus positive serum. Trade Science Inc. - INDIA 2015 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/8406/1/Expression.pdf Magdline Sia, Henry Sum and Anna, Andrew (2015) Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus. BioTechnology an Indian Journal, 11 (3). ISSN 0974 - 7435 http://www.researchgate.net/profile/Magdline_Sia_Henry_Sum/publication/272634054_Expression_and_evaluation_of_a_24-kDa_recombinant_protein_of_the_N-terminal_E2_glycoprotein_of_chikungunya_virus/links/54eadfff0cf2f7aa4d581ee0.pdf |
| spellingShingle | R Medicine (General) Magdline Sia, Henry Sum Anna, Andrew Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus |
| title | Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus |
| title_full | Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus |
| title_fullStr | Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus |
| title_full_unstemmed | Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus |
| title_short | Expression and evaluation of a 24-kDa recombinant protein of the N-terminal E2 glycoprotein of chikungunya virus |
| title_sort | expression and evaluation of a 24-kda recombinant protein of the n-terminal e2 glycoprotein of chikungunya virus |
| topic | R Medicine (General) |
| url | http://ir.unimas.my/id/eprint/8406/ http://ir.unimas.my/id/eprint/8406/ http://ir.unimas.my/id/eprint/8406/1/Expression.pdf |