Variability of Dominant Endophytic Fungi Associated with Nypa fruticans and Selected Water Parameters in Kuching Wetland National Park (KWNP), Sarawak
A research was done in Kuching Wetland National Park (KWNP), Sarawak to analyse the basic physicochemical water properties of KWNP, the dominancy of endophytic fungi that associated with Nypa fruticans as well as to relate the dominant endophytic fungi with respects to the variability of the water...
| Main Author: | |
|---|---|
| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2014
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/8338/ http://ir.unimas.my/id/eprint/8338/1/Variability%20of%20Dominant%20Endophytic%20Fungi%20Associated%20with%20Nypa%20fruticans%20and%20Selected%20Water%20Parameters%20in%20Kuching%20Wetland%20National%20Park%20%28KWNP%29%2C%20Sarawak%20%2824pgs%29.pdf http://ir.unimas.my/id/eprint/8338/2/Variability%20of%20Dominant%20Endophytic%20Fungi%20Associated%20with%20Nypa%20fruticans%20and%20Selected%20Water%20Parameters%20in%20Kuching%20Wetland%20National%20Park%20%28KWNP%29%2C%20Sarawak%20%28full%29.pdf |
| _version_ | 1848836360824160256 |
|---|---|
| author | Nuraini, Binti Mohd Sabri |
| author_facet | Nuraini, Binti Mohd Sabri |
| author_sort | Nuraini, Binti Mohd Sabri |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | A research was done in Kuching Wetland National Park (KWNP), Sarawak to analyse the basic physicochemical
water properties of KWNP, the dominancy of endophytic fungi that associated with Nypa fruticans
as well as to relate the dominant endophytic fungi with respects to the variability of the water parameters.
The present study involved the combination of water parameters assessments, fungal screening in heavy
metals and molecular techniques. Sampling was carried out on September 2013 and selected water
parameters; pH, temperature, salinity, turbidity, total suspended solids and nutrients were analysed. A sample
of Nypa fruticans from each sampling stations were taken for endophytic fungi works. The range of
environmental conditions; pH, temperature, salinity, turbidity and total suspended solids are 8.0 to 8.6,28.29
to 29.49 °C, 7 to 22 PSU, 833.7 to 1561.07 NTU and 0.017 to 0.123 mg/L, respectively. Based on the result
ofwater parameters, five representative sites were chosen. Endophytic fungal isolates from these five stations
were screened in two concentrations (500 ppm and 1000 ppm) of four heavy metals; Zinc (Zn), Lead (Pb),
Copper (Co) and Chromium (Cr)to select the dominant fungal isolates. A total of 8 isolates were able to
grow, three isolates can resist in Zn, Pb and Co while the other five isolates can resists in Zn, Pb and Cr.
Molecular techniques done using Internal Transcribed Spacer (ITS), ITS 1 and ITS4 as primers. Phylogenetic
tree constructed supported the morphological identification of Isolate 2, 3, 4, 6, 7 and 8, which is
Pestalotiopsis sp. Pestalotiopsis sp were identified to have high tolerance on wide environmental conditions.
Future research on morphology should be done using Scanning Electron Microscope or Transmission
Electron Microscope to further distinguish and correctly defined the species ofendophytic fungi. |
| first_indexed | 2025-11-15T06:22:32Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-8338 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:22:32Z |
| publishDate | 2014 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-83382015-09-14T01:14:26Z http://ir.unimas.my/id/eprint/8338/ Variability of Dominant Endophytic Fungi Associated with Nypa fruticans and Selected Water Parameters in Kuching Wetland National Park (KWNP), Sarawak Nuraini, Binti Mohd Sabri GE Environmental Sciences A research was done in Kuching Wetland National Park (KWNP), Sarawak to analyse the basic physicochemical water properties of KWNP, the dominancy of endophytic fungi that associated with Nypa fruticans as well as to relate the dominant endophytic fungi with respects to the variability of the water parameters. The present study involved the combination of water parameters assessments, fungal screening in heavy metals and molecular techniques. Sampling was carried out on September 2013 and selected water parameters; pH, temperature, salinity, turbidity, total suspended solids and nutrients were analysed. A sample of Nypa fruticans from each sampling stations were taken for endophytic fungi works. The range of environmental conditions; pH, temperature, salinity, turbidity and total suspended solids are 8.0 to 8.6,28.29 to 29.49 °C, 7 to 22 PSU, 833.7 to 1561.07 NTU and 0.017 to 0.123 mg/L, respectively. Based on the result ofwater parameters, five representative sites were chosen. Endophytic fungal isolates from these five stations were screened in two concentrations (500 ppm and 1000 ppm) of four heavy metals; Zinc (Zn), Lead (Pb), Copper (Co) and Chromium (Cr)to select the dominant fungal isolates. A total of 8 isolates were able to grow, three isolates can resist in Zn, Pb and Co while the other five isolates can resists in Zn, Pb and Cr. Molecular techniques done using Internal Transcribed Spacer (ITS), ITS 1 and ITS4 as primers. Phylogenetic tree constructed supported the morphological identification of Isolate 2, 3, 4, 6, 7 and 8, which is Pestalotiopsis sp. Pestalotiopsis sp were identified to have high tolerance on wide environmental conditions. Future research on morphology should be done using Scanning Electron Microscope or Transmission Electron Microscope to further distinguish and correctly defined the species ofendophytic fungi. Universiti Malaysia Sarawak, (UNIMAS) 2014 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/8338/1/Variability%20of%20Dominant%20Endophytic%20Fungi%20Associated%20with%20Nypa%20fruticans%20and%20Selected%20Water%20Parameters%20in%20Kuching%20Wetland%20National%20Park%20%28KWNP%29%2C%20Sarawak%20%2824pgs%29.pdf text en http://ir.unimas.my/id/eprint/8338/2/Variability%20of%20Dominant%20Endophytic%20Fungi%20Associated%20with%20Nypa%20fruticans%20and%20Selected%20Water%20Parameters%20in%20Kuching%20Wetland%20National%20Park%20%28KWNP%29%2C%20Sarawak%20%28full%29.pdf Nuraini, Binti Mohd Sabri (2014) Variability of Dominant Endophytic Fungi Associated with Nypa fruticans and Selected Water Parameters in Kuching Wetland National Park (KWNP), Sarawak. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | GE Environmental Sciences Nuraini, Binti Mohd Sabri Variability of Dominant Endophytic Fungi Associated with Nypa fruticans and Selected Water Parameters in Kuching Wetland National Park (KWNP), Sarawak |
| title | Variability of Dominant Endophytic Fungi Associated with Nypa fruticans and Selected Water Parameters in Kuching Wetland National Park (KWNP), Sarawak |
| title_full | Variability of Dominant Endophytic Fungi Associated with Nypa fruticans and Selected Water Parameters in Kuching Wetland National Park (KWNP), Sarawak |
| title_fullStr | Variability of Dominant Endophytic Fungi Associated with Nypa fruticans and Selected Water Parameters in Kuching Wetland National Park (KWNP), Sarawak |
| title_full_unstemmed | Variability of Dominant Endophytic Fungi Associated with Nypa fruticans and Selected Water Parameters in Kuching Wetland National Park (KWNP), Sarawak |
| title_short | Variability of Dominant Endophytic Fungi Associated with Nypa fruticans and Selected Water Parameters in Kuching Wetland National Park (KWNP), Sarawak |
| title_sort | variability of dominant endophytic fungi associated with nypa fruticans and selected water parameters in kuching wetland national park (kwnp), sarawak |
| topic | GE Environmental Sciences |
| url | http://ir.unimas.my/id/eprint/8338/ http://ir.unimas.my/id/eprint/8338/1/Variability%20of%20Dominant%20Endophytic%20Fungi%20Associated%20with%20Nypa%20fruticans%20and%20Selected%20Water%20Parameters%20in%20Kuching%20Wetland%20National%20Park%20%28KWNP%29%2C%20Sarawak%20%2824pgs%29.pdf http://ir.unimas.my/id/eprint/8338/2/Variability%20of%20Dominant%20Endophytic%20Fungi%20Associated%20with%20Nypa%20fruticans%20and%20Selected%20Water%20Parameters%20in%20Kuching%20Wetland%20National%20Park%20%28KWNP%29%2C%20Sarawak%20%28full%29.pdf |