Variability of Dominant Endophytic Fungi Associated with Nypa fruticans and Selected Water Parameters in Kuching Wetland National Park (KWNP), Sarawak
A research was done in Kuching Wetland National Park (KWNP), Sarawak to analyse the basic physicochemical water properties of KWNP, the dominancy of endophytic fungi that associated with Nypa fruticans as well as to relate the dominant endophytic fungi with respects to the variability of the water...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
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Universiti Malaysia Sarawak, (UNIMAS)
2014
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| Online Access: | http://ir.unimas.my/id/eprint/8338/ http://ir.unimas.my/id/eprint/8338/1/Variability%20of%20Dominant%20Endophytic%20Fungi%20Associated%20with%20Nypa%20fruticans%20and%20Selected%20Water%20Parameters%20in%20Kuching%20Wetland%20National%20Park%20%28KWNP%29%2C%20Sarawak%20%2824pgs%29.pdf http://ir.unimas.my/id/eprint/8338/2/Variability%20of%20Dominant%20Endophytic%20Fungi%20Associated%20with%20Nypa%20fruticans%20and%20Selected%20Water%20Parameters%20in%20Kuching%20Wetland%20National%20Park%20%28KWNP%29%2C%20Sarawak%20%28full%29.pdf |
| Summary: | A research was done in Kuching Wetland National Park (KWNP), Sarawak to analyse the basic physicochemical
water properties of KWNP, the dominancy of endophytic fungi that associated with Nypa fruticans
as well as to relate the dominant endophytic fungi with respects to the variability of the water parameters.
The present study involved the combination of water parameters assessments, fungal screening in heavy
metals and molecular techniques. Sampling was carried out on September 2013 and selected water
parameters; pH, temperature, salinity, turbidity, total suspended solids and nutrients were analysed. A sample
of Nypa fruticans from each sampling stations were taken for endophytic fungi works. The range of
environmental conditions; pH, temperature, salinity, turbidity and total suspended solids are 8.0 to 8.6,28.29
to 29.49 °C, 7 to 22 PSU, 833.7 to 1561.07 NTU and 0.017 to 0.123 mg/L, respectively. Based on the result
ofwater parameters, five representative sites were chosen. Endophytic fungal isolates from these five stations
were screened in two concentrations (500 ppm and 1000 ppm) of four heavy metals; Zinc (Zn), Lead (Pb),
Copper (Co) and Chromium (Cr)to select the dominant fungal isolates. A total of 8 isolates were able to
grow, three isolates can resist in Zn, Pb and Co while the other five isolates can resists in Zn, Pb and Cr.
Molecular techniques done using Internal Transcribed Spacer (ITS), ITS 1 and ITS4 as primers. Phylogenetic
tree constructed supported the morphological identification of Isolate 2, 3, 4, 6, 7 and 8, which is
Pestalotiopsis sp. Pestalotiopsis sp were identified to have high tolerance on wide environmental conditions.
Future research on morphology should be done using Scanning Electron Microscope or Transmission
Electron Microscope to further distinguish and correctly defined the species ofendophytic fungi. |
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