Molecular phylogeny of grouper based on cytochrome oxidase i gene analysis
Epinephelus sexfasciatlls (six bar grouper) is a reef-associated species that often known as seafood delicacy in Asian countries. This fish is well-distributed across western Pacific region. Limited molecular data is available for this species, but none came from Malaysia.This study is aimed to se...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2014
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| Online Access: | http://ir.unimas.my/id/eprint/8336/ http://ir.unimas.my/id/eprint/8336/8/Molecular%20Phylogeny%20of%20Grouper%20Based%20on%20Cytochrome%20Oxidase%20I%20Gene%20Analysis%20%28full%29.pdf |
| Summary: | Epinephelus sexfasciatlls (six bar grouper) is a reef-associated species that often known as seafood delicacy in
Asian countries. This fish is well-distributed across western Pacific region. Limited molecular data is available
for this species, but none came from Malaysia.This study is aimed to sequence COl gene from genus Epinephellls
ESM03 followed by determination of the genetic divergence values and phylogenetic analysis. This study
involved total genomic DNA extraction, optical density reading, agarose gel electrophoresis, polymerase chain
reaction and DNA sequencing as well as data analysis. A total of 496 bp of COl gene from grouper ESM03 had
been successfully sequenced and it matched 99% with E. sexfasciatus KC959953.1. Results obtained from this
gene showed that there are mutations present in certain base. Pairwise genetic divergence analysis revealed that
there is 2.6% of difference between grouper ESM03 and E. sexfasciatus KC959953.l. The phylogenetic trees
constructed showed that genus Epinephellis is monophyletic. |
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