Water quality and characteristics of bacteria from fish facility
Maintaining the quality and safety of the water is important as the contamination of the water can cause serious illness or even death among the creatures in the water. In this study, chemical and microbiological water quality was determined. Samples of water were collected from fish facility of D...
| Main Author: | |
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2012
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/8334/ http://ir.unimas.my/id/eprint/8334/4/Kong%20full.pdf |
| _version_ | 1848836359528120320 |
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| author | Kong, Chak Yien |
| author_facet | Kong, Chak Yien |
| author_sort | Kong, Chak Yien |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Maintaining the quality and safety of the water is important as the contamination of the water can cause
serious illness or even death among the creatures in the water. In this study, chemical and microbiological
water quality was determined. Samples of water were collected from fish facility of Department of Molecular
Biology, Faculty of Resource Science and Technology, UNIMAS. These water samples were analysed for
the presence of bacteria. Bacteria were isolated from the water and were characterized. The DNA extraction
of the bacterial isolates was performed by boiling-centrifugation method. The genetic variations among the
isolates were analysed by (GTG)5 PCR. (GTG)5 PCR analysis indicated that the isolates were grouped into 2
main clusters. Then, the isolates were picked from the dendrogram for PCR to amplify the 16S rRNA gene of
the isolates. Twelve isolates were chosen for sequencing analysis. Among the twelve isolates, eight isolates
were Aeromonas veronii, two isolates are Aeromonas jandaei, and the last two isolates are Plesiomonas
shigelloides and Pseudomonas alcaligenes respectively. All isolates from the water were tested for their
antibiotic resistance by disc diffusion method. AU of the isolates were tested to be resistant towards
ampicillin. penicillin and gentamicin. The MAR index of the isolates ranged from 0.455 to 0.727. The
multiple antibiotic resistances of the isolates indicate the high potential hazard associated with antibioticresistant
bacteria in water. |
| first_indexed | 2025-11-15T06:22:31Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-8334 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:22:31Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-83342023-08-11T03:31:04Z http://ir.unimas.my/id/eprint/8334/ Water quality and characteristics of bacteria from fish facility Kong, Chak Yien GE Environmental Sciences Maintaining the quality and safety of the water is important as the contamination of the water can cause serious illness or even death among the creatures in the water. In this study, chemical and microbiological water quality was determined. Samples of water were collected from fish facility of Department of Molecular Biology, Faculty of Resource Science and Technology, UNIMAS. These water samples were analysed for the presence of bacteria. Bacteria were isolated from the water and were characterized. The DNA extraction of the bacterial isolates was performed by boiling-centrifugation method. The genetic variations among the isolates were analysed by (GTG)5 PCR. (GTG)5 PCR analysis indicated that the isolates were grouped into 2 main clusters. Then, the isolates were picked from the dendrogram for PCR to amplify the 16S rRNA gene of the isolates. Twelve isolates were chosen for sequencing analysis. Among the twelve isolates, eight isolates were Aeromonas veronii, two isolates are Aeromonas jandaei, and the last two isolates are Plesiomonas shigelloides and Pseudomonas alcaligenes respectively. All isolates from the water were tested for their antibiotic resistance by disc diffusion method. AU of the isolates were tested to be resistant towards ampicillin. penicillin and gentamicin. The MAR index of the isolates ranged from 0.455 to 0.727. The multiple antibiotic resistances of the isolates indicate the high potential hazard associated with antibioticresistant bacteria in water. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/8334/4/Kong%20full.pdf Kong, Chak Yien (2012) Water quality and characteristics of bacteria from fish facility. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | GE Environmental Sciences Kong, Chak Yien Water quality and characteristics of bacteria from fish facility |
| title | Water quality and characteristics of bacteria from fish facility |
| title_full | Water quality and characteristics of bacteria from fish facility |
| title_fullStr | Water quality and characteristics of bacteria from fish facility |
| title_full_unstemmed | Water quality and characteristics of bacteria from fish facility |
| title_short | Water quality and characteristics of bacteria from fish facility |
| title_sort | water quality and characteristics of bacteria from fish facility |
| topic | GE Environmental Sciences |
| url | http://ir.unimas.my/id/eprint/8334/ http://ir.unimas.my/id/eprint/8334/4/Kong%20full.pdf |