Preliminary Genetic Analysis on Sea Cucumber From Sampadi Island, Sarawak
Sea cucumbers (Echinodermata: Holothuroidea) are also known as Holothuroids or Holothurians which play important role in coral reef ecosystem. They are well known as a medicinal properties and as a food source. A few molecular studies had been carried out in Malaysia mainly using 16S rRNA gene an...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
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Universiti Malaysia Sarawak, (UNIMAS)
2014
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| Online Access: | http://ir.unimas.my/id/eprint/8274/ http://ir.unimas.my/id/eprint/8274/1/Preliminary%20Genetic%20Analysis%20of%20Sea%20Cucumber%20From%20Sampadi%20Island%2C%20Sarawak%2824pgs%29.pdf http://ir.unimas.my/id/eprint/8274/2/Preliminary%20Genetic%20Analysis%20of%20Sea%20Cucumber%20From%20Sampadi%20Island%2C%20Sarawak.pdf |
| _version_ | 1848836341682405376 |
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| author | Nur Natasya, binti Ab Halim |
| author_facet | Nur Natasya, binti Ab Halim |
| author_sort | Nur Natasya, binti Ab Halim |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Sea cucumbers (Echinodermata: Holothuroidea) are also known as Holothuroids or Holothurians
which play important role in coral reef ecosystem. They are well known as a medicinal properties
and as a food source. A few molecular studies had been carried out in Malaysia mainly using 16S
rRNA gene and COI gene to study molecular phylogeny of Holothuroidea. However, there is no
study conducted on Holothuria from Sarawak. Therefore, this study is to sequence COI gene from
Holothuria of Sampadi Island and to assess genetic variation between individuals and infer
molecular phylogeny of Holothuria. A total of 406 bp of COI gene sequences were obtained from
H. leucospilota in this study. Genetic divergence of 1.1% to 2.8% was recorded among H.
leucospilota samples from this study and other H. leucospilota sequence data from Genbank thus
indicating intraspecific variations between H. leucospilota. Phylogenetic trees constructed using
Neighbour Joining (NJ) and Maximum Parsimony (MP) showed monophyletic of the H.
leucospilota as they grouped together into a single clade. Limited samples were used in this study.
Therefore, more samples from different locations should be used for future work. |
| first_indexed | 2025-11-15T06:22:14Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-8274 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:22:14Z |
| publishDate | 2014 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-82742015-09-14T01:05:06Z http://ir.unimas.my/id/eprint/8274/ Preliminary Genetic Analysis on Sea Cucumber From Sampadi Island, Sarawak Nur Natasya, binti Ab Halim GE Environmental Sciences Sea cucumbers (Echinodermata: Holothuroidea) are also known as Holothuroids or Holothurians which play important role in coral reef ecosystem. They are well known as a medicinal properties and as a food source. A few molecular studies had been carried out in Malaysia mainly using 16S rRNA gene and COI gene to study molecular phylogeny of Holothuroidea. However, there is no study conducted on Holothuria from Sarawak. Therefore, this study is to sequence COI gene from Holothuria of Sampadi Island and to assess genetic variation between individuals and infer molecular phylogeny of Holothuria. A total of 406 bp of COI gene sequences were obtained from H. leucospilota in this study. Genetic divergence of 1.1% to 2.8% was recorded among H. leucospilota samples from this study and other H. leucospilota sequence data from Genbank thus indicating intraspecific variations between H. leucospilota. Phylogenetic trees constructed using Neighbour Joining (NJ) and Maximum Parsimony (MP) showed monophyletic of the H. leucospilota as they grouped together into a single clade. Limited samples were used in this study. Therefore, more samples from different locations should be used for future work. Universiti Malaysia Sarawak, (UNIMAS) 2014 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/8274/1/Preliminary%20Genetic%20Analysis%20of%20Sea%20Cucumber%20From%20Sampadi%20Island%2C%20Sarawak%2824pgs%29.pdf text en http://ir.unimas.my/id/eprint/8274/2/Preliminary%20Genetic%20Analysis%20of%20Sea%20Cucumber%20From%20Sampadi%20Island%2C%20Sarawak.pdf Nur Natasya, binti Ab Halim (2014) Preliminary Genetic Analysis on Sea Cucumber From Sampadi Island, Sarawak. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | GE Environmental Sciences Nur Natasya, binti Ab Halim Preliminary Genetic Analysis on Sea Cucumber From Sampadi Island, Sarawak |
| title | Preliminary Genetic Analysis on Sea Cucumber From Sampadi Island, Sarawak |
| title_full | Preliminary Genetic Analysis on Sea Cucumber From Sampadi Island, Sarawak |
| title_fullStr | Preliminary Genetic Analysis on Sea Cucumber From Sampadi Island, Sarawak |
| title_full_unstemmed | Preliminary Genetic Analysis on Sea Cucumber From Sampadi Island, Sarawak |
| title_short | Preliminary Genetic Analysis on Sea Cucumber From Sampadi Island, Sarawak |
| title_sort | preliminary genetic analysis on sea cucumber from sampadi island, sarawak |
| topic | GE Environmental Sciences |
| url | http://ir.unimas.my/id/eprint/8274/ http://ir.unimas.my/id/eprint/8274/1/Preliminary%20Genetic%20Analysis%20of%20Sea%20Cucumber%20From%20Sampadi%20Island%2C%20Sarawak%2824pgs%29.pdf http://ir.unimas.my/id/eprint/8274/2/Preliminary%20Genetic%20Analysis%20of%20Sea%20Cucumber%20From%20Sampadi%20Island%2C%20Sarawak.pdf |