Preliminary Genetic Analysis on Sea Cucumber From Sampadi Island, Sarawak
Sea cucumbers (Echinodermata: Holothuroidea) are also known as Holothuroids or Holothurians which play important role in coral reef ecosystem. They are well known as a medicinal properties and as a food source. A few molecular studies had been carried out in Malaysia mainly using 16S rRNA gene an...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2014
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/8274/ http://ir.unimas.my/id/eprint/8274/1/Preliminary%20Genetic%20Analysis%20of%20Sea%20Cucumber%20From%20Sampadi%20Island%2C%20Sarawak%2824pgs%29.pdf http://ir.unimas.my/id/eprint/8274/2/Preliminary%20Genetic%20Analysis%20of%20Sea%20Cucumber%20From%20Sampadi%20Island%2C%20Sarawak.pdf |
| Summary: | Sea cucumbers (Echinodermata: Holothuroidea) are also known as Holothuroids or Holothurians
which play important role in coral reef ecosystem. They are well known as a medicinal properties
and as a food source. A few molecular studies had been carried out in Malaysia mainly using 16S
rRNA gene and COI gene to study molecular phylogeny of Holothuroidea. However, there is no
study conducted on Holothuria from Sarawak. Therefore, this study is to sequence COI gene from
Holothuria of Sampadi Island and to assess genetic variation between individuals and infer
molecular phylogeny of Holothuria. A total of 406 bp of COI gene sequences were obtained from
H. leucospilota in this study. Genetic divergence of 1.1% to 2.8% was recorded among H.
leucospilota samples from this study and other H. leucospilota sequence data from Genbank thus
indicating intraspecific variations between H. leucospilota. Phylogenetic trees constructed using
Neighbour Joining (NJ) and Maximum Parsimony (MP) showed monophyletic of the H.
leucospilota as they grouped together into a single clade. Limited samples were used in this study.
Therefore, more samples from different locations should be used for future work. |
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