Morphological and Molecular Characterisation of Water Lily (Family: Nymphaeaceae) in UNIMAS Lake East Campus
Water lilies are aquatic angiosperms in the Family Nymphaeaceae. They are important ornamental plants that enhance the beauty of ponds and lakes. Several studies on water lilies in Sarawak had been carried out on ecological aspects, but there is limited number of molecular data on water lilies in...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2014
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| Online Access: | http://ir.unimas.my/id/eprint/8267/ http://ir.unimas.my/id/eprint/8267/1/Morphological%20and%20Molecular%20Characterisation%20of%20Water%20Lily%20%28Family%20%20Nympheaceae%29%20in%20UNIMAS%20Lake%20East%20Campus%20%2824pgs%29.pdf http://ir.unimas.my/id/eprint/8267/2/Morphological%20and%20Molecular%20Characterisation%20of%20Water%20Lily%20%28Family%20%20Nympheaceae%29%20in%20UNIMAS%20Lake%20East%20Campus.pdf |
| Summary: | Water lilies are aquatic angiosperms in the Family Nymphaeaceae. They are important ornamental plants that
enhance the beauty of ponds and lakes. Several studies on water lilies in Sarawak had been carried out on
ecological aspects, but there is limited number of molecular data on water lilies in Malaysia available in the
GenBank. This study is designed to sequence the internal transcribed spacer (ITS) regions of nuclear
ribosomal DNA from the water lilies sampled from Universiti Malaysia Sarawak (UNIMAS) Lake, East
Campus. A total of 11 water lilies were subjected to identification based on morphological characteristics. In
addition, for molecular work, the total genomic DNA was extracted from the flowers and leaves using
modified CTAB method followed by Polymerase Chain Reaction to amplify 730 bp ITS gene, DNA
sequencing and data analysis. In this study, there were three species of water lily found in UNIMAS Lake
identified as Nymphaea capensis, N. lotus and N. pubescens based on combination of morphological data and
ITS gene sequence. N. capensis1 and N. capensis2 have low genetic divergent (0% - 0.9%) which suggesting
intraspecific variations. N. capensis and N. lotus have 30.7 % of genetic divergent whereas N. lotus and N.
pubescens have genetic divergent value of 5.5 %. N. lotus is monophyletic with high bootstrap value, 98% in
NJ tree and 97% in MP tree. N. capensis has bootstrap value of 99% in NJ tree and 100% in MP tree which
indicates that the species is monophyletic. N. pubescens is also monophyletic with 100% bootstrap value in
both NJ and MP tree. |
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