Horseshoe Crabs of Western Part of Sarawak Coastal Waters: Distribution and Preliminary Molecular Study

Horseshoe crabs are the oldest creature in the world and the concern for its status has increased. Present study was aimed to determine the distribution and preliminary genetic study of horseshoe crabs based on Cytochrome Oxidase I gene analysis at western part of Sarawak coastal waters. Field sam...

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Main Author: Amni Nabilah, binti Mat Adam
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2014
Subjects:
Online Access:http://ir.unimas.my/id/eprint/8264/
http://ir.unimas.my/id/eprint/8264/1/Amni.pdf
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author Amni Nabilah, binti Mat Adam
author_facet Amni Nabilah, binti Mat Adam
author_sort Amni Nabilah, binti Mat Adam
building UNIMAS Institutional Repository
collection Online Access
description Horseshoe crabs are the oldest creature in the world and the concern for its status has increased. Present study was aimed to determine the distribution and preliminary genetic study of horseshoe crabs based on Cytochrome Oxidase I gene analysis at western part of Sarawak coastal waters. Field sampling was carried out during range of new moon days between October 2013 and March 2014 in Sibu Laut (01°41’18.2” N, 110°12’08.9”E) and Kg. Pasir Putih (01°39’49.0” N, 110°28’30.0”E). A total of 104 horseshoe crabs were collected throughout sampling sites (Sibu Laut=18; Kg. Pasir Putih=86) which two species present, Tachypleus gigas and Carcinoscorpius rotundicauda. There was relationship between body weight and carapace width of T. gigas at Sibu Laut and Kg. Pasir Putih. All physicochemical parameters was significant (p<0.05) in both sampling sites. Mean percentage of mass particle showed the particle size of sand was mostly medium sand (42.935± 21.800 %) at Sibu Laut whereas fine sand (53.514±8.495 %) at Kg. Pasir Putih. A total of 611bp COI gene sequence was obtained from T. gigas, SL1a2 and it matched with sequence of T. gigas, JF896114. Based on COI gene sequence analysis, T. gigas from Sibu Laut and other T.gigas from GenBank sample was monophyletic and formed a clade with high bootstrap value
first_indexed 2025-11-15T06:22:11Z
format Final Year Project Report / IMRAD
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institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:22:11Z
publishDate 2014
publisher Universiti Malaysia Sarawak, (UNIMAS)
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spelling unimas-82642023-06-21T00:28:32Z http://ir.unimas.my/id/eprint/8264/ Horseshoe Crabs of Western Part of Sarawak Coastal Waters: Distribution and Preliminary Molecular Study Amni Nabilah, binti Mat Adam SH Aquaculture. Fisheries. Angling Horseshoe crabs are the oldest creature in the world and the concern for its status has increased. Present study was aimed to determine the distribution and preliminary genetic study of horseshoe crabs based on Cytochrome Oxidase I gene analysis at western part of Sarawak coastal waters. Field sampling was carried out during range of new moon days between October 2013 and March 2014 in Sibu Laut (01°41’18.2” N, 110°12’08.9”E) and Kg. Pasir Putih (01°39’49.0” N, 110°28’30.0”E). A total of 104 horseshoe crabs were collected throughout sampling sites (Sibu Laut=18; Kg. Pasir Putih=86) which two species present, Tachypleus gigas and Carcinoscorpius rotundicauda. There was relationship between body weight and carapace width of T. gigas at Sibu Laut and Kg. Pasir Putih. All physicochemical parameters was significant (p<0.05) in both sampling sites. Mean percentage of mass particle showed the particle size of sand was mostly medium sand (42.935± 21.800 %) at Sibu Laut whereas fine sand (53.514±8.495 %) at Kg. Pasir Putih. A total of 611bp COI gene sequence was obtained from T. gigas, SL1a2 and it matched with sequence of T. gigas, JF896114. Based on COI gene sequence analysis, T. gigas from Sibu Laut and other T.gigas from GenBank sample was monophyletic and formed a clade with high bootstrap value Universiti Malaysia Sarawak, (UNIMAS) 2014 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/8264/1/Amni.pdf Amni Nabilah, binti Mat Adam (2014) Horseshoe Crabs of Western Part of Sarawak Coastal Waters: Distribution and Preliminary Molecular Study. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle SH Aquaculture. Fisheries. Angling
Amni Nabilah, binti Mat Adam
Horseshoe Crabs of Western Part of Sarawak Coastal Waters: Distribution and Preliminary Molecular Study
title Horseshoe Crabs of Western Part of Sarawak Coastal Waters: Distribution and Preliminary Molecular Study
title_full Horseshoe Crabs of Western Part of Sarawak Coastal Waters: Distribution and Preliminary Molecular Study
title_fullStr Horseshoe Crabs of Western Part of Sarawak Coastal Waters: Distribution and Preliminary Molecular Study
title_full_unstemmed Horseshoe Crabs of Western Part of Sarawak Coastal Waters: Distribution and Preliminary Molecular Study
title_short Horseshoe Crabs of Western Part of Sarawak Coastal Waters: Distribution and Preliminary Molecular Study
title_sort horseshoe crabs of western part of sarawak coastal waters: distribution and preliminary molecular study
topic SH Aquaculture. Fisheries. Angling
url http://ir.unimas.my/id/eprint/8264/
http://ir.unimas.my/id/eprint/8264/1/Amni.pdf