Screening and characterization of fibrolytic bacteria from sago waste
A total of 16 bacteria were successfully isolated from the sago waste after selectively enriched in media containing 1 % (w/v) of corn cobs. Screening of the bacterial isolates was done by using Congo-red staining method after the bacterial strains was cultured onto the CMC agar plates and 1 % corn...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2012
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| Online Access: | http://ir.unimas.my/id/eprint/8222/ http://ir.unimas.my/id/eprint/8222/3/Almuhsen%20full.pdf |
| _version_ | 1848836327954448384 |
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| author | Mohd Almuhsen, Karim |
| author_facet | Mohd Almuhsen, Karim |
| author_sort | Mohd Almuhsen, Karim |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | A total of 16 bacteria were successfully isolated from the sago waste after selectively enriched in media containing 1 % (w/v) of corn cobs. Screening of the bacterial isolates was done by using Congo-red staining method after the bacterial strains was cultured onto the CMC agar plates and 1 % corn cobs agar plates, respectively. Four isolates were chosen and selected for further analysis due to their ability to produce large halo and characterized. Standard biochemical test, morphological characterization, cellulase enzyme assay, xylanase enzyme assay and molecular characterization were done on these four isolates. Enzyme assays were conducted using ONS method. After ONA sequencing with 16s rONA analysis, the isolates were found to be similar to Bacillus pumilus and Bacillus subtiUs. |
| first_indexed | 2025-11-15T06:22:01Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-8222 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:22:01Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-82222023-08-28T04:15:06Z http://ir.unimas.my/id/eprint/8222/ Screening and characterization of fibrolytic bacteria from sago waste Mohd Almuhsen, Karim Q Science (General) A total of 16 bacteria were successfully isolated from the sago waste after selectively enriched in media containing 1 % (w/v) of corn cobs. Screening of the bacterial isolates was done by using Congo-red staining method after the bacterial strains was cultured onto the CMC agar plates and 1 % corn cobs agar plates, respectively. Four isolates were chosen and selected for further analysis due to their ability to produce large halo and characterized. Standard biochemical test, morphological characterization, cellulase enzyme assay, xylanase enzyme assay and molecular characterization were done on these four isolates. Enzyme assays were conducted using ONS method. After ONA sequencing with 16s rONA analysis, the isolates were found to be similar to Bacillus pumilus and Bacillus subtiUs. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/8222/3/Almuhsen%20full.pdf Mohd Almuhsen, Karim (2012) Screening and characterization of fibrolytic bacteria from sago waste. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) Mohd Almuhsen, Karim Screening and characterization of fibrolytic bacteria from sago waste |
| title | Screening and characterization of fibrolytic bacteria from sago waste |
| title_full | Screening and characterization of fibrolytic bacteria from sago waste |
| title_fullStr | Screening and characterization of fibrolytic bacteria from sago waste |
| title_full_unstemmed | Screening and characterization of fibrolytic bacteria from sago waste |
| title_short | Screening and characterization of fibrolytic bacteria from sago waste |
| title_sort | screening and characterization of fibrolytic bacteria from sago waste |
| topic | Q Science (General) |
| url | http://ir.unimas.my/id/eprint/8222/ http://ir.unimas.my/id/eprint/8222/3/Almuhsen%20full.pdf |