Isolation and characterization of the ribosomal protein gene, RPL37A, from human nasopharyngeal-derived cell lines
Previous studies have shown that expression of RPL37A gene was downregulated in nasopharyngeal carcinoma cell lines and it had been reported that genetic mutation of coding sequences in ribosomal protein gene RPSJ9 is the cause of dysregulation of gene expression in Diamond-Blackfan Anemia. The o...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2012
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/8211/ http://ir.unimas.my/id/eprint/8211/11/Kiung%20Siong%20Siang%20%28full%29.pdf |
| _version_ | 1848836324708057088 |
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| author | Kiung, Siong Siang |
| author_facet | Kiung, Siong Siang |
| author_sort | Kiung, Siong Siang |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Previous studies have shown that expression of RPL37A gene was downregulated in
nasopharyngeal carcinoma cell lines and it had been reported that genetic mutation of
coding sequences in ribosomal protein gene RPSJ9 is the cause of dysregulation of gene
expression in Diamond-Blackfan Anemia. The objective of this study is to determine
whether mutation in coding sequences of RPL37A gene is the cause of its differential
expression in nasopharyngeal carcinoma by isolating, sequencing and analyzing the coding
sequences of the gene from normal nasopharyngeal epithelial cell lines and nasopharyngeal
carcinoma cell lines. Results from this study show that there is no difference in the
nucleotide sequence in the coding region of RPL37A gene from both types of
nasopharyngeal cell lines and therefore indicates that mutation in the coding region of the
RPL37A gene is not the cause of its differential expression in nasopharyngeal carcinoma. |
| first_indexed | 2025-11-15T06:21:58Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-8211 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:21:58Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-82112023-08-11T03:29:36Z http://ir.unimas.my/id/eprint/8211/ Isolation and characterization of the ribosomal protein gene, RPL37A, from human nasopharyngeal-derived cell lines Kiung, Siong Siang QM Human anatomy Previous studies have shown that expression of RPL37A gene was downregulated in nasopharyngeal carcinoma cell lines and it had been reported that genetic mutation of coding sequences in ribosomal protein gene RPSJ9 is the cause of dysregulation of gene expression in Diamond-Blackfan Anemia. The objective of this study is to determine whether mutation in coding sequences of RPL37A gene is the cause of its differential expression in nasopharyngeal carcinoma by isolating, sequencing and analyzing the coding sequences of the gene from normal nasopharyngeal epithelial cell lines and nasopharyngeal carcinoma cell lines. Results from this study show that there is no difference in the nucleotide sequence in the coding region of RPL37A gene from both types of nasopharyngeal cell lines and therefore indicates that mutation in the coding region of the RPL37A gene is not the cause of its differential expression in nasopharyngeal carcinoma. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/8211/11/Kiung%20Siong%20Siang%20%28full%29.pdf Kiung, Siong Siang (2012) Isolation and characterization of the ribosomal protein gene, RPL37A, from human nasopharyngeal-derived cell lines. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | QM Human anatomy Kiung, Siong Siang Isolation and characterization of the ribosomal protein gene, RPL37A, from human nasopharyngeal-derived cell lines |
| title | Isolation and characterization of the ribosomal protein gene, RPL37A, from human nasopharyngeal-derived cell lines |
| title_full | Isolation and characterization of the ribosomal protein gene, RPL37A, from human nasopharyngeal-derived cell lines |
| title_fullStr | Isolation and characterization of the ribosomal protein gene, RPL37A, from human nasopharyngeal-derived cell lines |
| title_full_unstemmed | Isolation and characterization of the ribosomal protein gene, RPL37A, from human nasopharyngeal-derived cell lines |
| title_short | Isolation and characterization of the ribosomal protein gene, RPL37A, from human nasopharyngeal-derived cell lines |
| title_sort | isolation and characterization of the ribosomal protein gene, rpl37a, from human nasopharyngeal-derived cell lines |
| topic | QM Human anatomy |
| url | http://ir.unimas.my/id/eprint/8211/ http://ir.unimas.my/id/eprint/8211/11/Kiung%20Siong%20Siang%20%28full%29.pdf |