Detection of L.Monocytogenes in animal and water samples in Kubah National Park, Kuching, Sarawak
Listeria monocytogenes (L. monocytogenes) is facultative anaerobic pathogens that can asymptomatically infect various animals. Normally, it also can be found in environment such as vegetation, water, soil, dust, mud and sewage. This study was conducted to detect the presence of L. monocytogenes an...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, (UNIMAS)
2012
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/8129/ http://ir.unimas.my/id/eprint/8129/1/Aimi%20Syazana%20Anuar%20ft.pdf |
| _version_ | 1848836307244023808 |
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| author | Aimi Syazana, Anuar |
| author_facet | Aimi Syazana, Anuar |
| author_sort | Aimi Syazana, Anuar |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Listeria monocytogenes (L. monocytogenes) is facultative anaerobic pathogens that can asymptomatically infect
various animals. Normally, it also can be found in environment such as vegetation, water, soil, dust, mud and
sewage. This study was conducted to detect the presence of L. monocytogenes and antimicrobial susceptibility
characteristic from wildlife and water samples at Kubah National Park, Kuching. Samples collected were
incubated and streaked on selective medium P ALCAM agar to confirm the presence of Listeria spp. before they
were further tested using biochemical and molecular analysis. Specific Polymerase Chain Reaction (PCR) assay
were performed to target specific virulence gene, such as haemolysin gene, h/yA to further distinguish the
presence ofthis pathogenic bacteria in the sample. The h/yA gene plays major roles in haemolytic properties in
L. monocytogenes as it produces Listeriolysin 0 (LLO) which involve actively in lysis process of phagocytic
membrane by releasing pore-forming toxin. Overall, out of the 30 samples tested, 10 samples were confirmed as
to contain L. monocytogenes isolates and selected to subsequent antimicrobial susceptibility test. The test
revealed that 90010 of the isolates were resistant to Tetracycline (30 ~g) and Erythromycin (30 Jlg) while
Gentamycin (10 Jlg) and Tobramycin (10 Jlg) has the lowest level of resistance (0%). The mUltiple antibiotic
resistances shown by L. monocytogenes isolates in this study indicate the potential health hazard associated with the possible transmission between wildlife and water to its surrounding environment especially visitors of
Kubah National Park, Kuching |
| first_indexed | 2025-11-15T06:21:41Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-8129 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:21:41Z |
| publishDate | 2012 |
| publisher | Universiti Malaysia Sarawak, (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-81292023-02-22T07:24:18Z http://ir.unimas.my/id/eprint/8129/ Detection of L.Monocytogenes in animal and water samples in Kubah National Park, Kuching, Sarawak Aimi Syazana, Anuar QR Microbiology Listeria monocytogenes (L. monocytogenes) is facultative anaerobic pathogens that can asymptomatically infect various animals. Normally, it also can be found in environment such as vegetation, water, soil, dust, mud and sewage. This study was conducted to detect the presence of L. monocytogenes and antimicrobial susceptibility characteristic from wildlife and water samples at Kubah National Park, Kuching. Samples collected were incubated and streaked on selective medium P ALCAM agar to confirm the presence of Listeria spp. before they were further tested using biochemical and molecular analysis. Specific Polymerase Chain Reaction (PCR) assay were performed to target specific virulence gene, such as haemolysin gene, h/yA to further distinguish the presence ofthis pathogenic bacteria in the sample. The h/yA gene plays major roles in haemolytic properties in L. monocytogenes as it produces Listeriolysin 0 (LLO) which involve actively in lysis process of phagocytic membrane by releasing pore-forming toxin. Overall, out of the 30 samples tested, 10 samples were confirmed as to contain L. monocytogenes isolates and selected to subsequent antimicrobial susceptibility test. The test revealed that 90010 of the isolates were resistant to Tetracycline (30 ~g) and Erythromycin (30 Jlg) while Gentamycin (10 Jlg) and Tobramycin (10 Jlg) has the lowest level of resistance (0%). The mUltiple antibiotic resistances shown by L. monocytogenes isolates in this study indicate the potential health hazard associated with the possible transmission between wildlife and water to its surrounding environment especially visitors of Kubah National Park, Kuching Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/8129/1/Aimi%20Syazana%20Anuar%20ft.pdf Aimi Syazana, Anuar (2012) Detection of L.Monocytogenes in animal and water samples in Kubah National Park, Kuching, Sarawak. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | QR Microbiology Aimi Syazana, Anuar Detection of L.Monocytogenes in animal and water samples in Kubah National Park, Kuching, Sarawak |
| title | Detection of L.Monocytogenes in animal and water samples in Kubah National Park, Kuching, Sarawak |
| title_full | Detection of L.Monocytogenes in animal and water samples in Kubah National Park, Kuching, Sarawak |
| title_fullStr | Detection of L.Monocytogenes in animal and water samples in Kubah National Park, Kuching, Sarawak |
| title_full_unstemmed | Detection of L.Monocytogenes in animal and water samples in Kubah National Park, Kuching, Sarawak |
| title_short | Detection of L.Monocytogenes in animal and water samples in Kubah National Park, Kuching, Sarawak |
| title_sort | detection of l.monocytogenes in animal and water samples in kubah national park, kuching, sarawak |
| topic | QR Microbiology |
| url | http://ir.unimas.my/id/eprint/8129/ http://ir.unimas.my/id/eprint/8129/1/Aimi%20Syazana%20Anuar%20ft.pdf |