Analysis of Putative Sago palm cysteine protease cDNA open reading frame
Cysteine protease is one of the types of proteolytic enzyme that present in Metroxylon sagu and has many roles in plant cells physiology and development process. In this study, the objective is to clone a putative cysteine protease gene derived from M. sagu into an expression vector. Firstly, the...
| Main Author: | |
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Faculty of Resource Science and Technology
2013
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| Online Access: | http://ir.unimas.my/id/eprint/8058/ http://ir.unimas.my/id/eprint/8058/3/Analysis%20of%20Putative%20Sago%20Palm%20Cysteine%20Protease%20cDNA%20Open%20Reading%20Frame.pdf |
| _version_ | 1848836289226342400 |
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| author | Jong, Thin Thin |
| author_facet | Jong, Thin Thin |
| author_sort | Jong, Thin Thin |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Cysteine protease is one of the types of proteolytic enzyme that present in Metroxylon sagu and has many
roles in plant cells physiology and development process. In this study, the objective is to clone a putative
cysteine protease gene derived from M. sagu into an expression vector. Firstly, the msCPR cDNA ORF
which has been isolated from sago palm, was propagated in pBluescript plasmid. The clone was analyzed via
restriction enzyme and PCR. The second objective is to clone the msCPR cDNA ORF into an expression
vector, pET-41a(+). The last objective is to determine the correct insertion of the coding fragment of pET-
41a(+) which containing msCPR cDNA ORF through DNA sequencing reaction. A pBluescript vector
containing the putative msCPR was checked via PCR and produced a fragment with the size of 750 bps. Then,
the pET-41a(+) vector and msCPR cDNA ORF was successfully digested with Nde I and Not I restriction
enzyme and subsequently ligated together. However, the determination of the correct insertion of the coding
fragment of pET-41a(+)-msCPR cDNA ORF through DNA sequencing reaction was failed to be conducted. |
| first_indexed | 2025-11-15T06:21:24Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-8058 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:21:24Z |
| publishDate | 2013 |
| publisher | Faculty of Resource Science and Technology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-80582023-06-28T08:19:28Z http://ir.unimas.my/id/eprint/8058/ Analysis of Putative Sago palm cysteine protease cDNA open reading frame Jong, Thin Thin Q Science (General) SB Plant culture Cysteine protease is one of the types of proteolytic enzyme that present in Metroxylon sagu and has many roles in plant cells physiology and development process. In this study, the objective is to clone a putative cysteine protease gene derived from M. sagu into an expression vector. Firstly, the msCPR cDNA ORF which has been isolated from sago palm, was propagated in pBluescript plasmid. The clone was analyzed via restriction enzyme and PCR. The second objective is to clone the msCPR cDNA ORF into an expression vector, pET-41a(+). The last objective is to determine the correct insertion of the coding fragment of pET- 41a(+) which containing msCPR cDNA ORF through DNA sequencing reaction. A pBluescript vector containing the putative msCPR was checked via PCR and produced a fragment with the size of 750 bps. Then, the pET-41a(+) vector and msCPR cDNA ORF was successfully digested with Nde I and Not I restriction enzyme and subsequently ligated together. However, the determination of the correct insertion of the coding fragment of pET-41a(+)-msCPR cDNA ORF through DNA sequencing reaction was failed to be conducted. Faculty of Resource Science and Technology 2013 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/8058/3/Analysis%20of%20Putative%20Sago%20Palm%20Cysteine%20Protease%20cDNA%20Open%20Reading%20Frame.pdf Jong, Thin Thin (2013) Analysis of Putative Sago palm cysteine protease cDNA open reading frame. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) SB Plant culture Jong, Thin Thin Analysis of Putative Sago palm cysteine protease cDNA open reading frame |
| title | Analysis of Putative Sago palm cysteine protease cDNA open reading frame |
| title_full | Analysis of Putative Sago palm cysteine protease cDNA open reading frame |
| title_fullStr | Analysis of Putative Sago palm cysteine protease cDNA open reading frame |
| title_full_unstemmed | Analysis of Putative Sago palm cysteine protease cDNA open reading frame |
| title_short | Analysis of Putative Sago palm cysteine protease cDNA open reading frame |
| title_sort | analysis of putative sago palm cysteine protease cdna open reading frame |
| topic | Q Science (General) SB Plant culture |
| url | http://ir.unimas.my/id/eprint/8058/ http://ir.unimas.my/id/eprint/8058/3/Analysis%20of%20Putative%20Sago%20Palm%20Cysteine%20Protease%20cDNA%20Open%20Reading%20Frame.pdf |