Screening for antibiotics in endophytes isolated from melastoma malabathricum from Lundu, Sarawak
A study was conducted to isolate and screen endophytes from Melastoma malabathricum for antibiotic activity. Ten samples from three plants were collected from Lundu, Sarawak, and used for the isolation. Of the ten fungal endophytes that were isolated, four fungal isolates, namely E1, E2, E3 and E4...
| Main Author: | |
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak (UNIMAS)
2010
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/7897/ http://ir.unimas.my/id/eprint/7897/3/Munirah%20full.pdf |
| _version_ | 1848836252100460544 |
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| author | Munirah, Abdul Talip |
| author_facet | Munirah, Abdul Talip |
| author_sort | Munirah, Abdul Talip |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | A study was conducted to isolate and screen endophytes from Melastoma malabathricum for antibiotic activity. Ten
samples from three plants were collected from Lundu, Sarawak, and used for the isolation. Of the ten fungal endophytes
that were isolated, four fungal isolates, namely E1, E2, E3 and E4, were considered separate isolates. In a preliminary
antimicrobial activity test using agar overlay technique, only isolates E2 and E3 showed zone of inhibition against test
bacteria. In this test, E2 was positive against both Gram positive bacteria, Staphylococcus aureus and Gram negative
bacteria, Escherichia coli. E3 showed positive result against Gram negative bacteria, E. coli. Further antimicrobial
activity test on E2 and E3 using agar overlay technique, revealed a contrasting results in which E2 was only positive
against S. aureus and that E3 was positive against E. coli. However, antifungal activity screening showed that all four
fungi isolates did not against the test fungus, which was Fusarium sp. Antibiotic screening test was also carried out on
methanol extracts of the four endophytes. In agar disc diffusion test, methanol extract of E2 gave positive result against
both S. aureus and Salmonella typhii. Methanol extract of E1 also gave positive result against S. aureus and S. typhii, but
the inhibition zone produced were not clearly shown. Methanol extracts of E3 and E4 were not active against the test
bacteria. Macroscopic and microscopic observations using slide culture technique showed that the four endophytic fungi
had different characteristics. The colonies of E1 and E4 were white, whereas E2 was purplish-brown, and E3 was grey in
colour. All three isolates E1, E2, and E3 possessed dark, septate, and brancing hyphae while E4 was hyaline, septate and
non-branching hyphae. The spores of E1 and E2 were both cylindrical in shape, while E3 and E4 did not sporulate. The
isolates E1 and E2 were tentatively identified as Fusarium sp. and Chalaropsis sp respectively. The E3 and E4 fungal
isolates were non-identified. |
| first_indexed | 2025-11-15T06:20:48Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-7897 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:20:48Z |
| publishDate | 2010 |
| publisher | Universiti Malaysia Sarawak (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-78972023-08-29T09:24:53Z http://ir.unimas.my/id/eprint/7897/ Screening for antibiotics in endophytes isolated from melastoma malabathricum from Lundu, Sarawak Munirah, Abdul Talip SB Plant culture A study was conducted to isolate and screen endophytes from Melastoma malabathricum for antibiotic activity. Ten samples from three plants were collected from Lundu, Sarawak, and used for the isolation. Of the ten fungal endophytes that were isolated, four fungal isolates, namely E1, E2, E3 and E4, were considered separate isolates. In a preliminary antimicrobial activity test using agar overlay technique, only isolates E2 and E3 showed zone of inhibition against test bacteria. In this test, E2 was positive against both Gram positive bacteria, Staphylococcus aureus and Gram negative bacteria, Escherichia coli. E3 showed positive result against Gram negative bacteria, E. coli. Further antimicrobial activity test on E2 and E3 using agar overlay technique, revealed a contrasting results in which E2 was only positive against S. aureus and that E3 was positive against E. coli. However, antifungal activity screening showed that all four fungi isolates did not against the test fungus, which was Fusarium sp. Antibiotic screening test was also carried out on methanol extracts of the four endophytes. In agar disc diffusion test, methanol extract of E2 gave positive result against both S. aureus and Salmonella typhii. Methanol extract of E1 also gave positive result against S. aureus and S. typhii, but the inhibition zone produced were not clearly shown. Methanol extracts of E3 and E4 were not active against the test bacteria. Macroscopic and microscopic observations using slide culture technique showed that the four endophytic fungi had different characteristics. The colonies of E1 and E4 were white, whereas E2 was purplish-brown, and E3 was grey in colour. All three isolates E1, E2, and E3 possessed dark, septate, and brancing hyphae while E4 was hyaline, septate and non-branching hyphae. The spores of E1 and E2 were both cylindrical in shape, while E3 and E4 did not sporulate. The isolates E1 and E2 were tentatively identified as Fusarium sp. and Chalaropsis sp respectively. The E3 and E4 fungal isolates were non-identified. Universiti Malaysia Sarawak (UNIMAS) 2010 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7897/3/Munirah%20full.pdf Munirah, Abdul Talip (2010) Screening for antibiotics in endophytes isolated from melastoma malabathricum from Lundu, Sarawak. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | SB Plant culture Munirah, Abdul Talip Screening for antibiotics in endophytes isolated from melastoma malabathricum from Lundu, Sarawak |
| title | Screening for antibiotics in endophytes isolated from melastoma malabathricum from Lundu, Sarawak |
| title_full | Screening for antibiotics in endophytes isolated from melastoma malabathricum from Lundu, Sarawak |
| title_fullStr | Screening for antibiotics in endophytes isolated from melastoma malabathricum from Lundu, Sarawak |
| title_full_unstemmed | Screening for antibiotics in endophytes isolated from melastoma malabathricum from Lundu, Sarawak |
| title_short | Screening for antibiotics in endophytes isolated from melastoma malabathricum from Lundu, Sarawak |
| title_sort | screening for antibiotics in endophytes isolated from melastoma malabathricum from lundu, sarawak |
| topic | SB Plant culture |
| url | http://ir.unimas.my/id/eprint/7897/ http://ir.unimas.my/id/eprint/7897/3/Munirah%20full.pdf |