Screening and Isolation of Cyclodextrin Producing Fungi and Bacteria From Selected UNIMAS AAS Collection
Cyclodextrin (CD) is cyclic oligosaccharides consisting of α-1,4-glucosidic linkage produced by the reaction of cyclodextrin glycosyltransferase (CGTase EC 2.4.1.19) and starch. CGTase are usually produced by bacteria mostly from the Bacillus genera. There is no research done on fungi for production...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
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Universiti Malaysia Sarawak (UNIMAS)
2010
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| Online Access: | http://ir.unimas.my/id/eprint/7895/ http://ir.unimas.my/id/eprint/7895/1/Kathleen.pdf http://ir.unimas.my/id/eprint/7895/4/Kathleen%20Michelle%20Mikal%20ft.pdf |
| _version_ | 1848836251592949760 |
|---|---|
| author | Kathleen, Michelle Mikal |
| author_facet | Kathleen, Michelle Mikal |
| author_sort | Kathleen, Michelle Mikal |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Cyclodextrin (CD) is cyclic oligosaccharides consisting of α-1,4-glucosidic linkage produced by the reaction of cyclodextrin glycosyltransferase (CGTase EC 2.4.1.19) and starch. CGTase are usually produced by bacteria mostly from the Bacillus genera. There is no research done on fungi for production of CD. In this study, screening was done on fungi and bacteria to identify which fungi and bacteria could produce CGTase that will later form CD. The screening was done on the Horikoshi II media containing phenolphthalein and methyl orange as CGTase indicator. Five fungi and three bacteria from the AAS UNIMAS collection were screened. Screening and enzyme assay showed that two fungi, Aspergillus nomius and Aspergillus flavus produce CGTase. Both fungi have high enzyme activity with 12.97U/ml for A.nomius and 10.66U/ml for A.flavus based on CGTase assay. |
| first_indexed | 2025-11-15T06:20:48Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-7895 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:20:48Z |
| publishDate | 2010 |
| publisher | Universiti Malaysia Sarawak (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-78952023-09-27T08:46:27Z http://ir.unimas.my/id/eprint/7895/ Screening and Isolation of Cyclodextrin Producing Fungi and Bacteria From Selected UNIMAS AAS Collection Kathleen, Michelle Mikal Q Science (General) Cyclodextrin (CD) is cyclic oligosaccharides consisting of α-1,4-glucosidic linkage produced by the reaction of cyclodextrin glycosyltransferase (CGTase EC 2.4.1.19) and starch. CGTase are usually produced by bacteria mostly from the Bacillus genera. There is no research done on fungi for production of CD. In this study, screening was done on fungi and bacteria to identify which fungi and bacteria could produce CGTase that will later form CD. The screening was done on the Horikoshi II media containing phenolphthalein and methyl orange as CGTase indicator. Five fungi and three bacteria from the AAS UNIMAS collection were screened. Screening and enzyme assay showed that two fungi, Aspergillus nomius and Aspergillus flavus produce CGTase. Both fungi have high enzyme activity with 12.97U/ml for A.nomius and 10.66U/ml for A.flavus based on CGTase assay. Universiti Malaysia Sarawak (UNIMAS) 2010 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7895/1/Kathleen.pdf text en http://ir.unimas.my/id/eprint/7895/4/Kathleen%20Michelle%20Mikal%20ft.pdf Kathleen, Michelle Mikal (2010) Screening and Isolation of Cyclodextrin Producing Fungi and Bacteria From Selected UNIMAS AAS Collection. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) Kathleen, Michelle Mikal Screening and Isolation of Cyclodextrin Producing Fungi and Bacteria From Selected UNIMAS AAS Collection |
| title | Screening and Isolation of Cyclodextrin Producing Fungi and Bacteria From Selected UNIMAS AAS Collection |
| title_full | Screening and Isolation of Cyclodextrin Producing Fungi and Bacteria From Selected UNIMAS AAS Collection |
| title_fullStr | Screening and Isolation of Cyclodextrin Producing Fungi and Bacteria From Selected UNIMAS AAS Collection |
| title_full_unstemmed | Screening and Isolation of Cyclodextrin Producing Fungi and Bacteria From Selected UNIMAS AAS Collection |
| title_short | Screening and Isolation of Cyclodextrin Producing Fungi and Bacteria From Selected UNIMAS AAS Collection |
| title_sort | screening and isolation of cyclodextrin producing fungi and bacteria from selected unimas aas collection |
| topic | Q Science (General) |
| url | http://ir.unimas.my/id/eprint/7895/ http://ir.unimas.my/id/eprint/7895/1/Kathleen.pdf http://ir.unimas.my/id/eprint/7895/4/Kathleen%20Michelle%20Mikal%20ft.pdf |