Screening and Isolation of Cyclodextin Glucanotransferase Producing Fungi From Selected UNIMAS AAS Collection

Cyclodextrin (CDs) is a cyclic homogenous oligosaccharide, synthesized from starch by an enzyme called cyclodextrin glucanotransferase (CGTase, EC 2.4. 1. 19) via a process called intramolecular transglycosylation in microorganism. CGTase is produced mainly by bacteria and recent finding shows that...

Full description

Bibliographic Details
Main Author: Amirah, Ahmad
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, UNIMAS 2010
Subjects:
Online Access:http://ir.unimas.my/id/eprint/7894/
http://ir.unimas.my/id/eprint/7894/1/Amirah%20Ahmad%20ft.pdf
_version_ 1848836251327660032
author Amirah, Ahmad
author_facet Amirah, Ahmad
author_sort Amirah, Ahmad
building UNIMAS Institutional Repository
collection Online Access
description Cyclodextrin (CDs) is a cyclic homogenous oligosaccharide, synthesized from starch by an enzyme called cyclodextrin glucanotransferase (CGTase, EC 2.4. 1. 19) via a process called intramolecular transglycosylation in microorganism. CGTase is produced mainly by bacteria and recent finding shows that archae is also able to produce CGTase. However, there is no study conducted on fungi to determine its potential as CGTase producer. Screening of CGTase producing fungi was done on samples obtained from UNIMAS AAS fungi collection. Rapid screening was done by using Horikoshi media with phenolphthalein and methyl orange. Sample SW005 which is Aspergillus sp. showed the best CGTase activity during screening compared to other sample as well as having the highest enzyme activity based on phenolphthalein assay which measure β-CD forming activity.
first_indexed 2025-11-15T06:20:48Z
format Final Year Project Report / IMRAD
id unimas-7894
institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:20:48Z
publishDate 2010
publisher Universiti Malaysia Sarawak, UNIMAS
recordtype eprints
repository_type Digital Repository
spelling unimas-78942023-02-27T04:24:24Z http://ir.unimas.my/id/eprint/7894/ Screening and Isolation of Cyclodextin Glucanotransferase Producing Fungi From Selected UNIMAS AAS Collection Amirah, Ahmad Q Science (General) Cyclodextrin (CDs) is a cyclic homogenous oligosaccharide, synthesized from starch by an enzyme called cyclodextrin glucanotransferase (CGTase, EC 2.4. 1. 19) via a process called intramolecular transglycosylation in microorganism. CGTase is produced mainly by bacteria and recent finding shows that archae is also able to produce CGTase. However, there is no study conducted on fungi to determine its potential as CGTase producer. Screening of CGTase producing fungi was done on samples obtained from UNIMAS AAS fungi collection. Rapid screening was done by using Horikoshi media with phenolphthalein and methyl orange. Sample SW005 which is Aspergillus sp. showed the best CGTase activity during screening compared to other sample as well as having the highest enzyme activity based on phenolphthalein assay which measure β-CD forming activity. Universiti Malaysia Sarawak, UNIMAS 2010 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7894/1/Amirah%20Ahmad%20ft.pdf Amirah, Ahmad (2010) Screening and Isolation of Cyclodextin Glucanotransferase Producing Fungi From Selected UNIMAS AAS Collection. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle Q Science (General)
Amirah, Ahmad
Screening and Isolation of Cyclodextin Glucanotransferase Producing Fungi From Selected UNIMAS AAS Collection
title Screening and Isolation of Cyclodextin Glucanotransferase Producing Fungi From Selected UNIMAS AAS Collection
title_full Screening and Isolation of Cyclodextin Glucanotransferase Producing Fungi From Selected UNIMAS AAS Collection
title_fullStr Screening and Isolation of Cyclodextin Glucanotransferase Producing Fungi From Selected UNIMAS AAS Collection
title_full_unstemmed Screening and Isolation of Cyclodextin Glucanotransferase Producing Fungi From Selected UNIMAS AAS Collection
title_short Screening and Isolation of Cyclodextin Glucanotransferase Producing Fungi From Selected UNIMAS AAS Collection
title_sort screening and isolation of cyclodextin glucanotransferase producing fungi from selected unimas aas collection
topic Q Science (General)
url http://ir.unimas.my/id/eprint/7894/
http://ir.unimas.my/id/eprint/7894/1/Amirah%20Ahmad%20ft.pdf