Molecular Characterization and Similarity Relationships Among Selected Kelampayan (Neolamarchia cadamba) Genotypes from Kelampayan Provenance Trial Plot I Using ISSR Markers

Neolamarckia cadamba or Kelampayan is a fast growing timber species that has economical and medicinal value. Kelampayan is one of the species widely used for reforestation programme, so as high commercial value in plywood manufacturing and wound cleansing. Therefore, molecular characterization of th...

Full description

Bibliographic Details
Main Author: Tiong, Shing Yiing
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, UNIMAS 2010
Subjects:
Online Access:http://ir.unimas.my/id/eprint/7877/
http://ir.unimas.my/id/eprint/7877/4/Shing%20Yiing%28fulltext%29.pdf
_version_ 1848836246002991104
author Tiong, Shing Yiing
author_facet Tiong, Shing Yiing
author_sort Tiong, Shing Yiing
building UNIMAS Institutional Repository
collection Online Access
description Neolamarckia cadamba or Kelampayan is a fast growing timber species that has economical and medicinal value. Kelampayan is one of the species widely used for reforestation programme, so as high commercial value in plywood manufacturing and wound cleansing. Therefore, molecular characterization of this species is important to enable further species improvement. In this project, determination of genetic relatedness and diversity of kelampayan was accomplish using ISSR primers namely (GTG)6, (AC)10 and (AG)10. Selected primers had successful amplified 64 loci with 73.44% of polymorphic in block I population. Shannon’s diversity index of all three combined primes was reported to be 0.350. Molecular diversity based on Jaccard’s similarity coefficient of 79 trees was in the ranged of 0.14 to 0.94. UPGMA dendrograms and neighbor joining tree were also constructed to show the genetic relationship of N. cadamba trees. In the outcome of the dendrograms reveals that, a total of four clusters were grouped for block 1 kelampayan trees indicating that N. cadamba trees are genetically diverse.
first_indexed 2025-11-15T06:20:43Z
format Final Year Project Report / IMRAD
id unimas-7877
institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:20:43Z
publishDate 2010
publisher Universiti Malaysia Sarawak, UNIMAS
recordtype eprints
repository_type Digital Repository
spelling unimas-78772024-08-26T08:11:57Z http://ir.unimas.my/id/eprint/7877/ Molecular Characterization and Similarity Relationships Among Selected Kelampayan (Neolamarchia cadamba) Genotypes from Kelampayan Provenance Trial Plot I Using ISSR Markers Tiong, Shing Yiing SB Plant culture Neolamarckia cadamba or Kelampayan is a fast growing timber species that has economical and medicinal value. Kelampayan is one of the species widely used for reforestation programme, so as high commercial value in plywood manufacturing and wound cleansing. Therefore, molecular characterization of this species is important to enable further species improvement. In this project, determination of genetic relatedness and diversity of kelampayan was accomplish using ISSR primers namely (GTG)6, (AC)10 and (AG)10. Selected primers had successful amplified 64 loci with 73.44% of polymorphic in block I population. Shannon’s diversity index of all three combined primes was reported to be 0.350. Molecular diversity based on Jaccard’s similarity coefficient of 79 trees was in the ranged of 0.14 to 0.94. UPGMA dendrograms and neighbor joining tree were also constructed to show the genetic relationship of N. cadamba trees. In the outcome of the dendrograms reveals that, a total of four clusters were grouped for block 1 kelampayan trees indicating that N. cadamba trees are genetically diverse. Universiti Malaysia Sarawak, UNIMAS 2010 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7877/4/Shing%20Yiing%28fulltext%29.pdf Tiong, Shing Yiing (2010) Molecular Characterization and Similarity Relationships Among Selected Kelampayan (Neolamarchia cadamba) Genotypes from Kelampayan Provenance Trial Plot I Using ISSR Markers. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle SB Plant culture
Tiong, Shing Yiing
Molecular Characterization and Similarity Relationships Among Selected Kelampayan (Neolamarchia cadamba) Genotypes from Kelampayan Provenance Trial Plot I Using ISSR Markers
title Molecular Characterization and Similarity Relationships Among Selected Kelampayan (Neolamarchia cadamba) Genotypes from Kelampayan Provenance Trial Plot I Using ISSR Markers
title_full Molecular Characterization and Similarity Relationships Among Selected Kelampayan (Neolamarchia cadamba) Genotypes from Kelampayan Provenance Trial Plot I Using ISSR Markers
title_fullStr Molecular Characterization and Similarity Relationships Among Selected Kelampayan (Neolamarchia cadamba) Genotypes from Kelampayan Provenance Trial Plot I Using ISSR Markers
title_full_unstemmed Molecular Characterization and Similarity Relationships Among Selected Kelampayan (Neolamarchia cadamba) Genotypes from Kelampayan Provenance Trial Plot I Using ISSR Markers
title_short Molecular Characterization and Similarity Relationships Among Selected Kelampayan (Neolamarchia cadamba) Genotypes from Kelampayan Provenance Trial Plot I Using ISSR Markers
title_sort molecular characterization and similarity relationships among selected kelampayan (neolamarchia cadamba) genotypes from kelampayan provenance trial plot i using issr markers
topic SB Plant culture
url http://ir.unimas.my/id/eprint/7877/
http://ir.unimas.my/id/eprint/7877/4/Shing%20Yiing%28fulltext%29.pdf