Isolation and characterization of domestic grease degrading bacteria from household greywater system

Twelve isolates of possible grease degrading bacteria were successfully obtained from domestic household greywater after selection process using minimal salt medium with additional 3% (v/v) domestic oils. Among these isolates, three samples were randomly chose based on lipase plate assay activity...

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Main Author: George, Deng Anyie.
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, UNIMAS 2007
Subjects:
Online Access:http://ir.unimas.my/id/eprint/7864/
http://ir.unimas.my/id/eprint/7864/3/George%20Deng%20A.pdf
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author George, Deng Anyie.
author_facet George, Deng Anyie.
author_sort George, Deng Anyie.
building UNIMAS Institutional Repository
collection Online Access
description Twelve isolates of possible grease degrading bacteria were successfully obtained from domestic household greywater after selection process using minimal salt medium with additional 3% (v/v) domestic oils. Among these isolates, three samples were randomly chose based on lipase plate assay activity for further analysis through molecular identification. The three samples were identified as Pseudomonas fulgida strain iCTE16, Pseudomonas fulgida strain iCTE 12, and Pseudomonas mephitica DNA strain ATCC 33665T. Additional test was made on the samples that gave positive result in lipase plate assay activity. Microscopic observation and Pseudomonas selective media (centrimide) further indicated that the identified samples are a Pseudomonas sp. The samples were tested on their rate in grease degrading capabilities. Pseudomonas fulgida strain iCTE 12 was the isolate that has the highest value in grease degrading activity.
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spelling unimas-78642023-10-03T06:36:43Z http://ir.unimas.my/id/eprint/7864/ Isolation and characterization of domestic grease degrading bacteria from household greywater system George, Deng Anyie. QC Physics QH301 Biology Twelve isolates of possible grease degrading bacteria were successfully obtained from domestic household greywater after selection process using minimal salt medium with additional 3% (v/v) domestic oils. Among these isolates, three samples were randomly chose based on lipase plate assay activity for further analysis through molecular identification. The three samples were identified as Pseudomonas fulgida strain iCTE16, Pseudomonas fulgida strain iCTE 12, and Pseudomonas mephitica DNA strain ATCC 33665T. Additional test was made on the samples that gave positive result in lipase plate assay activity. Microscopic observation and Pseudomonas selective media (centrimide) further indicated that the identified samples are a Pseudomonas sp. The samples were tested on their rate in grease degrading capabilities. Pseudomonas fulgida strain iCTE 12 was the isolate that has the highest value in grease degrading activity. Universiti Malaysia Sarawak, UNIMAS 2007 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7864/3/George%20Deng%20A.pdf George, Deng Anyie. (2007) Isolation and characterization of domestic grease degrading bacteria from household greywater system. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle QC Physics
QH301 Biology
George, Deng Anyie.
Isolation and characterization of domestic grease degrading bacteria from household greywater system
title Isolation and characterization of domestic grease degrading bacteria from household greywater system
title_full Isolation and characterization of domestic grease degrading bacteria from household greywater system
title_fullStr Isolation and characterization of domestic grease degrading bacteria from household greywater system
title_full_unstemmed Isolation and characterization of domestic grease degrading bacteria from household greywater system
title_short Isolation and characterization of domestic grease degrading bacteria from household greywater system
title_sort isolation and characterization of domestic grease degrading bacteria from household greywater system
topic QC Physics
QH301 Biology
url http://ir.unimas.my/id/eprint/7864/
http://ir.unimas.my/id/eprint/7864/3/George%20Deng%20A.pdf