Hunting for second copy of GRB10 interacting gyf domain (GIGYF) family genes in danio rerio genome

Grb10 Interacting GYF domain (GIGYF) family genes consist of two members, namely GIGYF1 and GIGYF2 respectively. These two genes have been found to have interaction with Grb10 and Insulin-like Growth Factor I (IGF-I) as well as the insulin receptor. Further studies had shown that mutation of GIGYF2...

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Main Author: Foong, Pik Mun
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, UNIMAS 2010
Subjects:
Online Access:http://ir.unimas.my/id/eprint/7856/
http://ir.unimas.my/id/eprint/7856/1/Foong%20Pik%20Mun%20ft.pdf
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author Foong, Pik Mun
author_facet Foong, Pik Mun
author_sort Foong, Pik Mun
building UNIMAS Institutional Repository
collection Online Access
description Grb10 Interacting GYF domain (GIGYF) family genes consist of two members, namely GIGYF1 and GIGYF2 respectively. These two genes have been found to have interaction with Grb10 and Insulin-like Growth Factor I (IGF-I) as well as the insulin receptor. Further studies had shown that mutation of GIGYF2 in the PARK 11 locus might causes development of familial Parkinson’s disease (PD). Therefore, the aim of this study is to identify a second copy of GIGYF genes from the genome of Danio rerio (zebrafish), in which the first copy has been accomplished by Lee in year 2008. The presence of another copy would suggest the occurrence of genome duplication in zebrafish, and this would enable further study of the gene, especially in determine its mutational effect on PD. In this study, two copies of GIGYF1 genes have been found in LG7 and 10 respectively, while single copy of GIGYF2 was identified in LG2, which is designed as the original copy. BLAST search result is however, showed high possibility of second copy of GIGYF2 in the genome. Subsequent phylogenetics analysis suggested the evolutionary relationship between zebrafish and the homologous species. Paralogous relationship was identified between the two GIGYF1 copies. The three-dimensional structures of GIGYF family genes have been constructed through homology modelling using 3D-Jigsaw, and composite modelling through I-TASSER. The models are sent for function prediction and annotation. Further researches are recommended to perform verification study through experimental approaches.
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format Final Year Project Report / IMRAD
id unimas-7856
institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:20:37Z
publishDate 2010
publisher Universiti Malaysia Sarawak, UNIMAS
recordtype eprints
repository_type Digital Repository
spelling unimas-78562023-08-30T03:58:25Z http://ir.unimas.my/id/eprint/7856/ Hunting for second copy of GRB10 interacting gyf domain (GIGYF) family genes in danio rerio genome Foong, Pik Mun Q Science (General) Grb10 Interacting GYF domain (GIGYF) family genes consist of two members, namely GIGYF1 and GIGYF2 respectively. These two genes have been found to have interaction with Grb10 and Insulin-like Growth Factor I (IGF-I) as well as the insulin receptor. Further studies had shown that mutation of GIGYF2 in the PARK 11 locus might causes development of familial Parkinson’s disease (PD). Therefore, the aim of this study is to identify a second copy of GIGYF genes from the genome of Danio rerio (zebrafish), in which the first copy has been accomplished by Lee in year 2008. The presence of another copy would suggest the occurrence of genome duplication in zebrafish, and this would enable further study of the gene, especially in determine its mutational effect on PD. In this study, two copies of GIGYF1 genes have been found in LG7 and 10 respectively, while single copy of GIGYF2 was identified in LG2, which is designed as the original copy. BLAST search result is however, showed high possibility of second copy of GIGYF2 in the genome. Subsequent phylogenetics analysis suggested the evolutionary relationship between zebrafish and the homologous species. Paralogous relationship was identified between the two GIGYF1 copies. The three-dimensional structures of GIGYF family genes have been constructed through homology modelling using 3D-Jigsaw, and composite modelling through I-TASSER. The models are sent for function prediction and annotation. Further researches are recommended to perform verification study through experimental approaches. Universiti Malaysia Sarawak, UNIMAS 2010 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7856/1/Foong%20Pik%20Mun%20ft.pdf Foong, Pik Mun (2010) Hunting for second copy of GRB10 interacting gyf domain (GIGYF) family genes in danio rerio genome. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle Q Science (General)
Foong, Pik Mun
Hunting for second copy of GRB10 interacting gyf domain (GIGYF) family genes in danio rerio genome
title Hunting for second copy of GRB10 interacting gyf domain (GIGYF) family genes in danio rerio genome
title_full Hunting for second copy of GRB10 interacting gyf domain (GIGYF) family genes in danio rerio genome
title_fullStr Hunting for second copy of GRB10 interacting gyf domain (GIGYF) family genes in danio rerio genome
title_full_unstemmed Hunting for second copy of GRB10 interacting gyf domain (GIGYF) family genes in danio rerio genome
title_short Hunting for second copy of GRB10 interacting gyf domain (GIGYF) family genes in danio rerio genome
title_sort hunting for second copy of grb10 interacting gyf domain (gigyf) family genes in danio rerio genome
topic Q Science (General)
url http://ir.unimas.my/id/eprint/7856/
http://ir.unimas.my/id/eprint/7856/1/Foong%20Pik%20Mun%20ft.pdf