Heterologous expression of cellulase gene from bacillus Amyloliquefacians UMAS 1002 in Escherichia Coli BLl21 (DE3)
Cloning and expression of cellulase gene from B. amyloliquefaciens in E. coli BL21 (DE3) was attempted to optimize the activity of recombinant cellulase. PCR amplification of cellulase gene from Top10 cell was not successful although different approaches were carried out. Alternative method was take...
| Main Author: | Komathi, Balasupramaniam |
|---|---|
| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, UNIMAS
2010
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/7853/ http://ir.unimas.my/id/eprint/7853/1/Komathi%20Balasupramaniam%20ft.pdf |
Similar Items
Alpha-Amylase from Bacillus amyloliquefaciens UMAS 1002 Gene Characterization and Expression in Escherichia coli
by: Muhammad Suhaib, M. H., et al.
Published: (2010)
by: Muhammad Suhaib, M. H., et al.
Published: (2010)
Alpha-Amylase and Endoglucanase from Bacillus amyloliquefaciens umas 1002: Gene chaacterization and expression in Escherichia coli
by: Muhammad Suhaib, Mat Hussin
Published: (2013)
by: Muhammad Suhaib, Mat Hussin
Published: (2013)
Kinetic studies of the purified amylase and cellulase enzymes of bacillus amyloliquefaciens umas 1002 grown on Sago pith waste
by: Zahrihan Azura, Azahan
Published: (2004)
by: Zahrihan Azura, Azahan
Published: (2004)
Heterologous expression of xylanase gene from bacillus sp. in e. coli bl21 (DE3)
by: Arutchelvan, Rajamanikam
Published: (2010)
by: Arutchelvan, Rajamanikam
Published: (2010)
Isolation and characterization of amylase gene from bacillus amyloliquefaciens UMAS 1002
by: Aida Sabrina, Zainal.
Published: (2004)
by: Aida Sabrina, Zainal.
Published: (2004)
Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002
by: Muhammad Suhaib, M. H., et al.
Published: (2013)
by: Muhammad Suhaib, M. H., et al.
Published: (2013)
Heterologous expression of Bromelain in Escherichia coli
by: M., Bala, et al.
Published: (2011)
by: M., Bala, et al.
Published: (2011)
Enhancing alpha-amylase and cellulase in vivo enzyme expressions on sago pith residue using basillus amyloliquefaciens Umas 1002
by: Faisal Ali, Anwarali Khan
Published: (2004)
by: Faisal Ali, Anwarali Khan
Published: (2004)
Enhancing α-Amylase and Cellulase in vivo Enzyme Expressions on Sago Pith Residue Using Bacilllus amyloliquefaciens UMAS 1002
by: Faisal Ali, Anwarali Khan, et al.
Published: (2006)
by: Faisal Ali, Anwarali Khan, et al.
Published: (2006)
Heterologous expression of Xylanase gene from klebsiella pneumoniae in E.coli BL21 (DE3)
by: Suhaila, Bt Zainol
Published: (2012)
by: Suhaila, Bt Zainol
Published: (2012)
Trial Biodeinking of Mixed Office Paper Using the Crude Enzymes Produced By Bacillus amyloliquefaciens Umas 1002 and Bacillus licheniformis P1
by: Shahirah, Che Amat
Published: (2010)
by: Shahirah, Che Amat
Published: (2010)
Enzymatic characterization of heterologously expressed fungal β-glucosidase BGLII in Escherichia coli
by: Mohamad Sobri, Mohamad Farhan, et al.
Published: (2017)
by: Mohamad Sobri, Mohamad Farhan, et al.
Published: (2017)
Screening, isolation and molecular characterization of @-amylase enzyme from basillus amyloliquefaciens umas 1002
by: Husna, Hj. Mohd Noor.
Published: (2003)
by: Husna, Hj. Mohd Noor.
Published: (2003)
Heterologous Expression Of Xylanase Gene From Klebsiella Pneumoniae In E. Coli Bl21 (De3) For Potential Use In Green Technology
by: Mohd Hasnain, Hussain, et al.
Published: (2013)
by: Mohd Hasnain, Hussain, et al.
Published: (2013)
Expression of phytase From Mitsuokella jalaludinii in heterologous hosts and characterization of expressed protein from escherichia coli
by: Tan, Wan Qin
Published: (2013)
by: Tan, Wan Qin
Published: (2013)
Heterologous expression of CGT-BS gene improved recombinant cyclodextrin glycosyltransferase production from Escherichia coli
by: Nik Pa, Nik Ida Mardiana, et al.
Published: (2017)
by: Nik Pa, Nik Ida Mardiana, et al.
Published: (2017)
Statistical Optimization of Green Fluorescent Protein Production from Escherichia coli BL21(DE3)
by: Chew, Few Ne, et al.
Published: (2012)
by: Chew, Few Ne, et al.
Published: (2012)
Heterologous Expression Of Avian
Influenza A (H5n1) Neuraminidase In
Kluyveromyces Lactis And Escherichia Coli
by: Taufik, Noor Zafiah
Published: (2016)
by: Taufik, Noor Zafiah
Published: (2016)
Heterologous Expression Of
Violacein Biosynthetic Gene Cluster
From Chromobacterium Violaceum Strain
Mth01 In Escherichia Coli
by: Matthew Minggu, Matthlessa
Published: (2017)
by: Matthew Minggu, Matthlessa
Published: (2017)
Dissection of synechococcus rubisco large subunit sections involved in heterologous holoenzyme formation in escherichia coli
by: Ong, Wei Chi
Published: (2023)
by: Ong, Wei Chi
Published: (2023)
Uma cultura de colaboração entre professores e bibliotecários: uma orientação tutorial de estudantes de Radiologia em Moçambique
by: Antunes, M., et al.
Published: (2015)
by: Antunes, M., et al.
Published: (2015)
Construction and expression of synthetic gene encoding MPT64 as extracellular protein in Escherichia coli BL21 (DE3) expression system
by: Kusuma, Sri Agung Fitri, et al.
Published: (2018)
by: Kusuma, Sri Agung Fitri, et al.
Published: (2018)
One-step biotransformation of ferulic acid into biovanillin using recombinant Escherichia coli BL21 (DE3)
by: Zamzuri, Nur Ain
Published: (2016)
by: Zamzuri, Nur Ain
Published: (2016)
Understanding carbon flux of a heterologous metabolic pathway in Escherichia coli for sustainable methyl methacrylate biosynthesis
by: Tilley, Clemency
Published: (2022)
by: Tilley, Clemency
Published: (2022)
Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
by: Wan Siti Atikah, Wan Omar, et al.
Published: (2016)
by: Wan Siti Atikah, Wan Omar, et al.
Published: (2016)
Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
by: Wan Omar, Wan Siti Atikah, et al.
Published: (2016)
by: Wan Omar, Wan Siti Atikah, et al.
Published: (2016)
Heterologous Expression and Characterization of Plant Lipase LIP2 from Elaeis guineensis Jacq. Oil Palm Mesocarp in Escherichia coli
by: Mohd Hadzdee, M. D., et al.
Published: (2021)
by: Mohd Hadzdee, M. D., et al.
Published: (2021)
Heterologous expression and characterization of plant lipase LIP2 from Elaeis guineensis Jacq. oil palm Mesocarp in Escherichia coli
by: Mohd Din, Mohd Hadzdee, et al.
Published: (2021)
by: Mohd Din, Mohd Hadzdee, et al.
Published: (2021)
Optimization of culture conditions for Mpt64 synthetic gene expression in Escherichia coli BL21 (DE3) using surface response methodology
by: Kusuma, Sri Agung Fitri, et al.
Published: (2019)
by: Kusuma, Sri Agung Fitri, et al.
Published: (2019)
Expression of influenza M2e-NP recombinant fusion protein in Escherichia coli BL21 (DE3) and its binding to antibodies
by: Tan, Mei Peng, et al.
Published: (2022)
by: Tan, Mei Peng, et al.
Published: (2022)
Production Of Novel Recombinant Antipfhrp2 VNAR-G1 Protein Using Escherichia Coli Bl21(DE3) Expression System
by: Kok, Boon Hui
Published: (2020)
by: Kok, Boon Hui
Published: (2020)
Production Of Novel Recombinant Antipfhrp2
Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System
by: Kok, Boon Hui
Published: (2020)
by: Kok, Boon Hui
Published: (2020)
Production Of Novel Recombinant Anti-PFHRP2 VNAR-G1 Protein Using Escherichia
COLI BL21(DE3) Expression System
by: Kok, Boon Hui
Published: (2020)
by: Kok, Boon Hui
Published: (2020)
Recovery of recombinant bromelain from Escherichia
coli BL21-AI
by: Bala, Muntari, et al.
Published: (2011)
by: Bala, Muntari, et al.
Published: (2011)
Overview on the employment of the lambda pL/cI587 regulation system in the overproduction of heterologous protein in escherichia coli culture
by: Razali, Firdausi, et al.
Published: (2004)
by: Razali, Firdausi, et al.
Published: (2004)
Secretory overexpression of CGTase gene from bacillus sp. G1 in escherichia coli
by: Lo, Po Kim, et al.
Published: (2005)
by: Lo, Po Kim, et al.
Published: (2005)
Comparison of purification methods to purify recombinant bromelain from Escherichia coli BL21-A1 = Perbandingan kaedah penulenan rekombinan bromelain daripada Escherichia coli BL21-A1
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2017)
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2017)
Heterologous expression and secretion of Bacillus coagulans ST-6 xylanase in Lactococcus lactis NZ9000
by: Roslan, Abdullah Munir
Published: (2017)
by: Roslan, Abdullah Munir
Published: (2017)
Improving of pelB-Secreted MPT64 protein released by Escherichia coli BL21 (DE3) using Triton X-100 and Tween-80
by: Kusuma, Sri Agung Fitri, et al.
Published: (2022)
by: Kusuma, Sri Agung Fitri, et al.
Published: (2022)
Engineering Escherichia coli for hyper-production of outer membrane vesicles enriched in heterologous lipidated antigens for use as a novel mucosal vaccine platform
by: Barbora, Martinkova
Published: (2023)
by: Barbora, Martinkova
Published: (2023)
Similar Items
-
Alpha-Amylase from Bacillus amyloliquefaciens UMAS 1002 Gene Characterization and Expression in Escherichia coli
by: Muhammad Suhaib, M. H., et al.
Published: (2010) -
Alpha-Amylase and Endoglucanase from Bacillus amyloliquefaciens umas 1002: Gene chaacterization and expression in Escherichia coli
by: Muhammad Suhaib, Mat Hussin
Published: (2013) -
Kinetic studies of the purified amylase and cellulase enzymes of bacillus amyloliquefaciens umas 1002 grown on Sago pith waste
by: Zahrihan Azura, Azahan
Published: (2004) -
Heterologous expression of xylanase gene from bacillus sp. in e. coli bl21 (DE3)
by: Arutchelvan, Rajamanikam
Published: (2010) -
Isolation and characterization of amylase gene from bacillus amyloliquefaciens UMAS 1002
by: Aida Sabrina, Zainal.
Published: (2004)