Cryopreservation of Excised-Embryos of Jatropha Curcas L. Using Vitrification Technique
A study was carried out to evaluate ability of preserving excised-embryos of Jatropha curcas L. in liquid nitrogen using vitrification technique. The initial moisture content and germination of excised-embryos of J.curcas was 4.38 percent and 92.5 percent, respectively. The excised-embryos were load...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, UNIMAS
2010
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| Online Access: | http://ir.unimas.my/id/eprint/7825/ http://ir.unimas.my/id/eprint/7825/1/Kristina%20Ahong%20ft.pdf |
| _version_ | 1848836231306149888 |
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| author | Kristina, Ahong |
| author_facet | Kristina, Ahong |
| author_sort | Kristina, Ahong |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | A study was carried out to evaluate ability of preserving excised-embryos of Jatropha curcas L. in liquid nitrogen using vitrification technique. The initial moisture content and germination of excised-embryos of J.curcas was 4.38 percent and 92.5 percent, respectively. The excised-embryos were loaded in different sucrose concentrations of 0.0, 0.2, 0.4, 0.6, 0.8 and 1.0M prior to treatment with Plant Vitrification Solution 2 (PVS2) for the period of 0, 30, 60, 90 and 120 minutes. Pretreatment using 0.4M sucrose with PVS2 for the period of 30 minutes gave the highest germination of 80 percent and 35.49 percent moisture content. The percentage of moisture content and germination decreased with increased in sucrose concentration and duration of exposure. Regression analysis showed that excised-embryos of J.curcas can be preserved in liquid nitrogen after vitrification for 37 days or 5 weeks and 2 days. |
| first_indexed | 2025-11-15T06:20:28Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-7825 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:20:28Z |
| publishDate | 2010 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-78252023-09-27T03:46:54Z http://ir.unimas.my/id/eprint/7825/ Cryopreservation of Excised-Embryos of Jatropha Curcas L. Using Vitrification Technique Kristina, Ahong Q Science (General) A study was carried out to evaluate ability of preserving excised-embryos of Jatropha curcas L. in liquid nitrogen using vitrification technique. The initial moisture content and germination of excised-embryos of J.curcas was 4.38 percent and 92.5 percent, respectively. The excised-embryos were loaded in different sucrose concentrations of 0.0, 0.2, 0.4, 0.6, 0.8 and 1.0M prior to treatment with Plant Vitrification Solution 2 (PVS2) for the period of 0, 30, 60, 90 and 120 minutes. Pretreatment using 0.4M sucrose with PVS2 for the period of 30 minutes gave the highest germination of 80 percent and 35.49 percent moisture content. The percentage of moisture content and germination decreased with increased in sucrose concentration and duration of exposure. Regression analysis showed that excised-embryos of J.curcas can be preserved in liquid nitrogen after vitrification for 37 days or 5 weeks and 2 days. Universiti Malaysia Sarawak, UNIMAS 2010 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7825/1/Kristina%20Ahong%20ft.pdf Kristina, Ahong (2010) Cryopreservation of Excised-Embryos of Jatropha Curcas L. Using Vitrification Technique. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) Kristina, Ahong Cryopreservation of Excised-Embryos of Jatropha Curcas L. Using Vitrification Technique |
| title | Cryopreservation of Excised-Embryos of Jatropha Curcas L. Using Vitrification Technique |
| title_full | Cryopreservation of Excised-Embryos of Jatropha Curcas L. Using Vitrification Technique |
| title_fullStr | Cryopreservation of Excised-Embryos of Jatropha Curcas L. Using Vitrification Technique |
| title_full_unstemmed | Cryopreservation of Excised-Embryos of Jatropha Curcas L. Using Vitrification Technique |
| title_short | Cryopreservation of Excised-Embryos of Jatropha Curcas L. Using Vitrification Technique |
| title_sort | cryopreservation of excised-embryos of jatropha curcas l. using vitrification technique |
| topic | Q Science (General) |
| url | http://ir.unimas.my/id/eprint/7825/ http://ir.unimas.my/id/eprint/7825/1/Kristina%20Ahong%20ft.pdf |