Comparative analysis of chloroplast DNA of crytocoryne species
This study focuses on determining polymorphism of chloroplast DNA sequence in Cryptocoryne species. The emergence of new hybrid of C. purpurea in Sarawak and Borneo creates attention among scientists to determine possible mother plants for the species. Determination of possible mother plants for the...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, UNIMAS
2010
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| Online Access: | http://ir.unimas.my/id/eprint/7824/ http://ir.unimas.my/id/eprint/7824/3/Comparative%20Analysis%20of%20Chloroplast%20DNA%20of%20Cryptocoryne%20Species.pdf |
| _version_ | 1848836230960119808 |
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| author | Muhammad Mustaqim, Haji Shamsudin |
| author_facet | Muhammad Mustaqim, Haji Shamsudin |
| author_sort | Muhammad Mustaqim, Haji Shamsudin |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | This study focuses on determining polymorphism of chloroplast DNA sequence in Cryptocoryne species. The emergence of new hybrid of C. purpurea in Sarawak and Borneo creates attention among scientists to determine possible mother plants for the species. Determination of possible mother plants for the hybrid through molecular work provides useful information for taxonomy study. One new hybrid of C. purpurea and three possible mother plants of the hybrid; C. cordata var. zonata, C. ferruginea and C. pallidinervia have been used. Polymorphism in chloroplast DNA in these Cryptocoryne species is determined through PCR amplification of rbcL gene sequence coded rubisco synthesis using primers rbcL-1 (5’-TGTCACCAAAAACAGAGACT-3’) and rbcL-2 (5’-TTCCATACTTCACAAGCAGC-3’), and restriction fragment digestion using several restriction endonucleases. Polymorphisms of certain restriction sites were obtained. |
| first_indexed | 2025-11-15T06:20:28Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-7824 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:20:28Z |
| publishDate | 2010 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-78242023-08-29T08:39:53Z http://ir.unimas.my/id/eprint/7824/ Comparative analysis of chloroplast DNA of crytocoryne species Muhammad Mustaqim, Haji Shamsudin Q Science (General) This study focuses on determining polymorphism of chloroplast DNA sequence in Cryptocoryne species. The emergence of new hybrid of C. purpurea in Sarawak and Borneo creates attention among scientists to determine possible mother plants for the species. Determination of possible mother plants for the hybrid through molecular work provides useful information for taxonomy study. One new hybrid of C. purpurea and three possible mother plants of the hybrid; C. cordata var. zonata, C. ferruginea and C. pallidinervia have been used. Polymorphism in chloroplast DNA in these Cryptocoryne species is determined through PCR amplification of rbcL gene sequence coded rubisco synthesis using primers rbcL-1 (5’-TGTCACCAAAAACAGAGACT-3’) and rbcL-2 (5’-TTCCATACTTCACAAGCAGC-3’), and restriction fragment digestion using several restriction endonucleases. Polymorphisms of certain restriction sites were obtained. Universiti Malaysia Sarawak, UNIMAS 2010 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7824/3/Comparative%20Analysis%20of%20Chloroplast%20DNA%20of%20Cryptocoryne%20Species.pdf Muhammad Mustaqim, Haji Shamsudin (2010) Comparative analysis of chloroplast DNA of crytocoryne species. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) Muhammad Mustaqim, Haji Shamsudin Comparative analysis of chloroplast DNA of crytocoryne species |
| title | Comparative analysis of chloroplast DNA of crytocoryne species |
| title_full | Comparative analysis of chloroplast DNA of crytocoryne species |
| title_fullStr | Comparative analysis of chloroplast DNA of crytocoryne species |
| title_full_unstemmed | Comparative analysis of chloroplast DNA of crytocoryne species |
| title_short | Comparative analysis of chloroplast DNA of crytocoryne species |
| title_sort | comparative analysis of chloroplast dna of crytocoryne species |
| topic | Q Science (General) |
| url | http://ir.unimas.my/id/eprint/7824/ http://ir.unimas.my/id/eprint/7824/3/Comparative%20Analysis%20of%20Chloroplast%20DNA%20of%20Cryptocoryne%20Species.pdf |