Isolation of vibrio parahaemolyticus from raw vegetables in local markets at Kuching and Samarahan
Currently, Vibrio parahaemolyticus are considered as one of the main cause for bacterial gastroenteristis worldwide. Various researches had been conducted and stated that the outbreaks involving raw fruits and vegetables occurred besides those of animal and seafood originated products. This study...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
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Universiti Malaysia Sarawak (UNIMAS)
2009
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| Online Access: | http://ir.unimas.my/id/eprint/7721/ http://ir.unimas.my/id/eprint/7721/1/Ling.pdf http://ir.unimas.my/id/eprint/7721/2/Ling%20full.pdf |
| Summary: | Currently, Vibrio parahaemolyticus are considered as one of the main cause for bacterial gastroenteristis worldwide. Various
researches had been conducted and stated that the outbreaks involving raw fruits and vegetables occurred besides those of
animal and seafood originated products. This study aims to detect and identify the presence of Vibrio parahaemolyticus in raw vegetables found in local wet markets, Kuching, Sarawak. In this study, samplings were conducted from three different stall
found from three different wet markets (Stutong, 7th Mile and Samarindah) respectively. Three types of vegetables were
bought and sampled, namely cucumber, tomato and water spinach. Enrichment was done in alkaline peptone water (APW).
Thiosulfate-citrate-bile salt (TCBS) agar and CHROMagar Vibrio was applied in the methodology in selection of Vibrio
parahaemolyticus.The bacteria appeared as green colonies on TCBS agar and purple on CHROMagar Vibrio. The
presumptive colonies were then be streaked on nutrient agar and later stored as stock culture. Biochemical tests were
performed in order to identify the isolated colonies. Vibrio parahaemolyticus was found in raw vegetables samples obtained
from local wet markets but in little quantity (20 occurrences from 270 total samples or 10.7% isolated from TCBS and 9
occurrences from 270 total samples or 3.3% isolated from CHROMagar Vibrio due to the poor hygiene level in the markets as well as improper handling of vegetables and cross-contamination with other food sold in the market. |
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