Clearance rate of two commercially important bivalves feeding on pseudo-nitzschia species (Bacillariophyceae)
The chain-forming pennate diatom, Pseudo-nitzschia is a cosmopolitan diatom. Contamination of domoic acid (DA), derived from several toxic species of Pseudo-nitzschia in filtered feeding bivalves, has subsequently caused seafood poisoning which is commonly known as amnesic shellfish poisoning (ASP)....
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, UNIMAS
2009
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| Online Access: | http://ir.unimas.my/id/eprint/7704/ http://ir.unimas.my/id/eprint/7704/8/TAN%20TOH%20HII.pdf |
| _version_ | 1848836195125035008 |
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| author | Tan, Toh Hii |
| author_facet | Tan, Toh Hii |
| author_sort | Tan, Toh Hii |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | The chain-forming pennate diatom, Pseudo-nitzschia is a cosmopolitan diatom. Contamination of domoic acid (DA), derived from several toxic species of Pseudo-nitzschia in filtered feeding bivalves, has subsequently caused seafood poisoning which is commonly known as amnesic shellfish poisoning (ASP). In this study, a feeding experiment was carried out to determine the clearance rates (CR) of two commercial bivalves, blood cockles, Anadara granosa and benthic clams, Polymesoda expansa feeding on Pseudo-nitzschia species. Mass clonal culture of Pseudo-nitzschia sp. strain PnMt43 from Muara Tebas was used as prey in this experiment. The bivalves were fed with different initial cell density ranging from 100 to 5000 cell mL-1. Subsamples were taken half-hourly for a period of six hours for cell counts. Our results showed two distinct clearance responses between the two bivalves (P = 0.0020). P. expansa had the higher CR with 7.316 cell gram-1 mL-1 day than A.granosa with 0.389 cell gram-1 mL-1. The half-saturation concentrations (K) for A. granosa and P. expansa were 955 and 437 cell mL-1, respectively. The higher clearance rate of P. expansa indicates the tendency to accumulate higher level of toxin during a Pseudo-nitzschia bloom. However, P. expansa with higher CR has lower K due to the difference in consumption pattern. Simulation of toxin kinetic of the two bivalves was not able to be conducted due to the absence of toxic Pseudo-nitzschia strains. Further studies on feeding responses and toxin accumulation-depuration rates are important to understand the mechanism of toxin kinetic of DA in these two commercially important bivalves. |
| first_indexed | 2025-11-15T06:19:54Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-7704 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:19:54Z |
| publishDate | 2009 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-77042024-08-27T08:14:50Z http://ir.unimas.my/id/eprint/7704/ Clearance rate of two commercially important bivalves feeding on pseudo-nitzschia species (Bacillariophyceae) Tan, Toh Hii GE Environmental Sciences The chain-forming pennate diatom, Pseudo-nitzschia is a cosmopolitan diatom. Contamination of domoic acid (DA), derived from several toxic species of Pseudo-nitzschia in filtered feeding bivalves, has subsequently caused seafood poisoning which is commonly known as amnesic shellfish poisoning (ASP). In this study, a feeding experiment was carried out to determine the clearance rates (CR) of two commercial bivalves, blood cockles, Anadara granosa and benthic clams, Polymesoda expansa feeding on Pseudo-nitzschia species. Mass clonal culture of Pseudo-nitzschia sp. strain PnMt43 from Muara Tebas was used as prey in this experiment. The bivalves were fed with different initial cell density ranging from 100 to 5000 cell mL-1. Subsamples were taken half-hourly for a period of six hours for cell counts. Our results showed two distinct clearance responses between the two bivalves (P = 0.0020). P. expansa had the higher CR with 7.316 cell gram-1 mL-1 day than A.granosa with 0.389 cell gram-1 mL-1. The half-saturation concentrations (K) for A. granosa and P. expansa were 955 and 437 cell mL-1, respectively. The higher clearance rate of P. expansa indicates the tendency to accumulate higher level of toxin during a Pseudo-nitzschia bloom. However, P. expansa with higher CR has lower K due to the difference in consumption pattern. Simulation of toxin kinetic of the two bivalves was not able to be conducted due to the absence of toxic Pseudo-nitzschia strains. Further studies on feeding responses and toxin accumulation-depuration rates are important to understand the mechanism of toxin kinetic of DA in these two commercially important bivalves. Universiti Malaysia Sarawak, UNIMAS 2009 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7704/8/TAN%20TOH%20HII.pdf Tan, Toh Hii (2009) Clearance rate of two commercially important bivalves feeding on pseudo-nitzschia species (Bacillariophyceae). [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | GE Environmental Sciences Tan, Toh Hii Clearance rate of two commercially important bivalves feeding on pseudo-nitzschia species (Bacillariophyceae) |
| title | Clearance rate of two commercially important bivalves feeding on pseudo-nitzschia species (Bacillariophyceae) |
| title_full | Clearance rate of two commercially important bivalves feeding on pseudo-nitzschia species (Bacillariophyceae) |
| title_fullStr | Clearance rate of two commercially important bivalves feeding on pseudo-nitzschia species (Bacillariophyceae) |
| title_full_unstemmed | Clearance rate of two commercially important bivalves feeding on pseudo-nitzschia species (Bacillariophyceae) |
| title_short | Clearance rate of two commercially important bivalves feeding on pseudo-nitzschia species (Bacillariophyceae) |
| title_sort | clearance rate of two commercially important bivalves feeding on pseudo-nitzschia species (bacillariophyceae) |
| topic | GE Environmental Sciences |
| url | http://ir.unimas.my/id/eprint/7704/ http://ir.unimas.my/id/eprint/7704/8/TAN%20TOH%20HII.pdf |