Sex identification in canarium odontophyllum miq. (Dabai) using random amplified polymorphic DNA (RAPD) marker
Canarium odontophyllum Miq. is a perennial plant native to Malaysia, Indonesia and Philippines. The pistillate plants bear fruits twice a year and are more preferable than staminate plants. Thus, sex determination in Canarium odontophyllum at seedling stage is very important for crop improvement pro...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, UNIMAS
2008
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| Online Access: | http://ir.unimas.my/id/eprint/7677/ http://ir.unimas.my/id/eprint/7677/8/Sex%20Identification%20in%20Canarium%20odontophyllum%20Miq.%20%28DABAI%29%20Using%20Random%20Amplified%20Polymorphic%20DNA%20%28RAPD%29%20Marker.pdf |
| _version_ | 1848836188063924224 |
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| author | Ting, Jen Ching |
| author_facet | Ting, Jen Ching |
| author_sort | Ting, Jen Ching |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Canarium odontophyllum Miq. is a perennial plant native to Malaysia, Indonesia and Philippines. The pistillate plants bear fruits twice a year and are more preferable than staminate plants. Thus, sex determination in Canarium odontophyllum at seedling stage is very important for crop improvement processes because it accelerates the identification of fruitful pistillate plants. In this study, Random Amplified Polymorphic DNA (RAPD) technique was used to amplify DNA segments, with the objective of finding molecular markers associated to sex determination locus in this species. DNA was isolated from one male and three female Dabai trees and subjected to RAPD analysis. Of 52 RAPD primers, one primer (OPL – 03) produced an approximately 1,000 bp amplification band detectable in all pistillate plants, while absent in the staminate plants. The exclusiveness of this female-specific diagnostic band could be used in a breeding program to screen the gender of Dabai trees long before they reach reproductive maturity, resulting in considerable savings of time and economic resources. |
| first_indexed | 2025-11-15T06:19:47Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-7677 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:19:47Z |
| publishDate | 2008 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-76772024-08-29T06:41:34Z http://ir.unimas.my/id/eprint/7677/ Sex identification in canarium odontophyllum miq. (Dabai) using random amplified polymorphic DNA (RAPD) marker Ting, Jen Ching S Agriculture (General) Canarium odontophyllum Miq. is a perennial plant native to Malaysia, Indonesia and Philippines. The pistillate plants bear fruits twice a year and are more preferable than staminate plants. Thus, sex determination in Canarium odontophyllum at seedling stage is very important for crop improvement processes because it accelerates the identification of fruitful pistillate plants. In this study, Random Amplified Polymorphic DNA (RAPD) technique was used to amplify DNA segments, with the objective of finding molecular markers associated to sex determination locus in this species. DNA was isolated from one male and three female Dabai trees and subjected to RAPD analysis. Of 52 RAPD primers, one primer (OPL – 03) produced an approximately 1,000 bp amplification band detectable in all pistillate plants, while absent in the staminate plants. The exclusiveness of this female-specific diagnostic band could be used in a breeding program to screen the gender of Dabai trees long before they reach reproductive maturity, resulting in considerable savings of time and economic resources. Universiti Malaysia Sarawak, UNIMAS 2008 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7677/8/Sex%20Identification%20in%20Canarium%20odontophyllum%20Miq.%20%28DABAI%29%20Using%20Random%20Amplified%20Polymorphic%20DNA%20%28RAPD%29%20Marker.pdf Ting, Jen Ching (2008) Sex identification in canarium odontophyllum miq. (Dabai) using random amplified polymorphic DNA (RAPD) marker. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | S Agriculture (General) Ting, Jen Ching Sex identification in canarium odontophyllum miq. (Dabai) using random amplified polymorphic DNA (RAPD) marker |
| title | Sex identification in canarium odontophyllum miq. (Dabai) using random amplified polymorphic DNA (RAPD) marker |
| title_full | Sex identification in canarium odontophyllum miq. (Dabai) using random amplified polymorphic DNA (RAPD) marker |
| title_fullStr | Sex identification in canarium odontophyllum miq. (Dabai) using random amplified polymorphic DNA (RAPD) marker |
| title_full_unstemmed | Sex identification in canarium odontophyllum miq. (Dabai) using random amplified polymorphic DNA (RAPD) marker |
| title_short | Sex identification in canarium odontophyllum miq. (Dabai) using random amplified polymorphic DNA (RAPD) marker |
| title_sort | sex identification in canarium odontophyllum miq. (dabai) using random amplified polymorphic dna (rapd) marker |
| topic | S Agriculture (General) |
| url | http://ir.unimas.my/id/eprint/7677/ http://ir.unimas.my/id/eprint/7677/8/Sex%20Identification%20in%20Canarium%20odontophyllum%20Miq.%20%28DABAI%29%20Using%20Random%20Amplified%20Polymorphic%20DNA%20%28RAPD%29%20Marker.pdf |