Risk assessment of gram-negative bacteria from food sources in Kota Samarahan and Satok
Forty six isolates of bacteria were isolated from food sources collected markets in Kota Samarahan and Satok. The food sources analysed were chicken meat, seafood and processed food. Out of the 46 isolates, 10 were randomly chosen and identified by 16S rRNA sequencing. The confirmed isolates were c...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, UNIMAS
2011
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| Online Access: | http://ir.unimas.my/id/eprint/7662/ http://ir.unimas.my/id/eprint/7662/4/Nurfarhana%20Kamez%20ft.pdf |
| _version_ | 1848836183780491264 |
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| author | Nurfarhana, Kamez |
| author_facet | Nurfarhana, Kamez |
| author_sort | Nurfarhana, Kamez |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Forty six isolates of bacteria were isolated from food sources collected markets in Kota Samarahan and Satok.
The food sources analysed were chicken meat, seafood and processed food. Out of the 46 isolates, 10 were randomly chosen and identified by 16S rRNA sequencing. The confirmed isolates were characterised by (GTG)5
PCR and antibiotic susceptibility testing. The 16S rRNA analysis revealed that the isolates were Klebsiella
pneumoniae, Klebsiella oxytoca, Proteus mirabilis, Enterobacter amnigenus, Enterobacter cloacae, and
Enterobacter cancerogenus. The phylogenetic analysis based on the (GTG)5 PCR showed that the isolates were
clustered into 3 main groups which were further subclustered into minor groups or individual isolates. The antibiotic resistance showed that the isolates were resistant towards ampicillin (10μg),
sulphamethoxazole/trimethoprim (25μg), carbenicillin (100μg), norfloxacin (10μg), chloramphenicol (30μg),
streptomycin (10μg), tetracycline (30μg), nitrofurantoin (300μg) and kanamycin (30μg). All of the isolates tested
were found to be sensitive towards nalidixic acid (30μg). The results of this study indicate the usefulness of the
16S rRNA in identifying the bacterial isolates and (GTG)5 PCR in grouping them. This study has revealed the
potential risk associated with antibiotic resistance bacteria from food sources. The results of this study suggested that the isolates were originated from high risk sources of contamination |
| first_indexed | 2025-11-15T06:19:43Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-7662 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:19:43Z |
| publishDate | 2011 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-76622023-11-29T08:37:54Z http://ir.unimas.my/id/eprint/7662/ Risk assessment of gram-negative bacteria from food sources in Kota Samarahan and Satok Nurfarhana, Kamez Q Science (General) QH301 Biology Forty six isolates of bacteria were isolated from food sources collected markets in Kota Samarahan and Satok. The food sources analysed were chicken meat, seafood and processed food. Out of the 46 isolates, 10 were randomly chosen and identified by 16S rRNA sequencing. The confirmed isolates were characterised by (GTG)5 PCR and antibiotic susceptibility testing. The 16S rRNA analysis revealed that the isolates were Klebsiella pneumoniae, Klebsiella oxytoca, Proteus mirabilis, Enterobacter amnigenus, Enterobacter cloacae, and Enterobacter cancerogenus. The phylogenetic analysis based on the (GTG)5 PCR showed that the isolates were clustered into 3 main groups which were further subclustered into minor groups or individual isolates. The antibiotic resistance showed that the isolates were resistant towards ampicillin (10μg), sulphamethoxazole/trimethoprim (25μg), carbenicillin (100μg), norfloxacin (10μg), chloramphenicol (30μg), streptomycin (10μg), tetracycline (30μg), nitrofurantoin (300μg) and kanamycin (30μg). All of the isolates tested were found to be sensitive towards nalidixic acid (30μg). The results of this study indicate the usefulness of the 16S rRNA in identifying the bacterial isolates and (GTG)5 PCR in grouping them. This study has revealed the potential risk associated with antibiotic resistance bacteria from food sources. The results of this study suggested that the isolates were originated from high risk sources of contamination Universiti Malaysia Sarawak, UNIMAS 2011 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7662/4/Nurfarhana%20Kamez%20ft.pdf Nurfarhana, Kamez (2011) Risk assessment of gram-negative bacteria from food sources in Kota Samarahan and Satok. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) QH301 Biology Nurfarhana, Kamez Risk assessment of gram-negative bacteria from food sources in Kota Samarahan and Satok |
| title | Risk assessment of gram-negative bacteria from food sources in Kota Samarahan and Satok |
| title_full | Risk assessment of gram-negative bacteria from food sources in Kota Samarahan and Satok |
| title_fullStr | Risk assessment of gram-negative bacteria from food sources in Kota Samarahan and Satok |
| title_full_unstemmed | Risk assessment of gram-negative bacteria from food sources in Kota Samarahan and Satok |
| title_short | Risk assessment of gram-negative bacteria from food sources in Kota Samarahan and Satok |
| title_sort | risk assessment of gram-negative bacteria from food sources in kota samarahan and satok |
| topic | Q Science (General) QH301 Biology |
| url | http://ir.unimas.my/id/eprint/7662/ http://ir.unimas.my/id/eprint/7662/4/Nurfarhana%20Kamez%20ft.pdf |