Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer
The genetic relatedness among Acacia superbulk (plus tree of Acacia mangium) and Acacia hybrid (Acacia mangium x Acacia auriculiformis) collected from the UNIMAS arboretum, Kota Samarahan, Sarawak was evaluated using M13 universal Primer. A total of 16 reproducible loci was generated where 56.3% of...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, UNIMAS
2008
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| Online Access: | http://ir.unimas.my/id/eprint/7655/ http://ir.unimas.my/id/eprint/7655/4/Suliana%28fulltext%29.pdf |
| _version_ | 1848836182123741184 |
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| author | Suliana, Charles Nyucong |
| author_facet | Suliana, Charles Nyucong |
| author_sort | Suliana, Charles Nyucong |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | The genetic relatedness among Acacia superbulk (plus tree of Acacia mangium) and Acacia hybrid (Acacia mangium x Acacia auriculiformis) collected from the UNIMAS arboretum, Kota Samarahan, Sarawak was evaluated using M13 universal Primer. A total of 16 reproducible loci was generated where 56.3% of the fragments were identified as polymorphic band with the size ranging from 500bp to 2.5kbp. An UPGMA dendrogram was constructed based on the Jaccard’s coefficient of similarity
matrix which then grouped the Acacia superbulk and Acacia hybrid into two main clusters, i.e. Cluster 1: Acacia superbulk trees and Cluster 2: Acacia hybrid trees. All Acacia hybrid trees showed identical banding profile and therefore, suggested that these trees were originated from the same clone, whereas Acacia superbulk trees were originated from randomly selected plus trees seeds of A. mangium. The preliminary results showed that M13 universal primer is a potential molecular marker
not only for assessing genetic relatedness but also could be used for clonal identification and seedling certification. However, further study by using other DAMD markers such as YNZ-22 and 33.6 can be carried out to validate these results. |
| first_indexed | 2025-11-15T06:19:42Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-7655 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:19:42Z |
| publishDate | 2008 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-76552024-02-28T06:23:01Z http://ir.unimas.my/id/eprint/7655/ Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer Suliana, Charles Nyucong Q Science (General) The genetic relatedness among Acacia superbulk (plus tree of Acacia mangium) and Acacia hybrid (Acacia mangium x Acacia auriculiformis) collected from the UNIMAS arboretum, Kota Samarahan, Sarawak was evaluated using M13 universal Primer. A total of 16 reproducible loci was generated where 56.3% of the fragments were identified as polymorphic band with the size ranging from 500bp to 2.5kbp. An UPGMA dendrogram was constructed based on the Jaccard’s coefficient of similarity matrix which then grouped the Acacia superbulk and Acacia hybrid into two main clusters, i.e. Cluster 1: Acacia superbulk trees and Cluster 2: Acacia hybrid trees. All Acacia hybrid trees showed identical banding profile and therefore, suggested that these trees were originated from the same clone, whereas Acacia superbulk trees were originated from randomly selected plus trees seeds of A. mangium. The preliminary results showed that M13 universal primer is a potential molecular marker not only for assessing genetic relatedness but also could be used for clonal identification and seedling certification. However, further study by using other DAMD markers such as YNZ-22 and 33.6 can be carried out to validate these results. Universiti Malaysia Sarawak, UNIMAS 2008 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7655/4/Suliana%28fulltext%29.pdf Suliana, Charles Nyucong (2008) Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) Suliana, Charles Nyucong Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer |
| title | Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer |
| title_full | Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer |
| title_fullStr | Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer |
| title_full_unstemmed | Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer |
| title_short | Evaluation of genetic relatedness among acacia superbulk and acacia hybrid (Acacia mangium x acacia auriculiformis) trees using M13 Universal Primer |
| title_sort | evaluation of genetic relatedness among acacia superbulk and acacia hybrid (acacia mangium x acacia auriculiformis) trees using m13 universal primer |
| topic | Q Science (General) |
| url | http://ir.unimas.my/id/eprint/7655/ http://ir.unimas.my/id/eprint/7655/4/Suliana%28fulltext%29.pdf |