Screening and isolation of amylolytic fungus and crude enzyme characterization from Sago industrial waste
Five best amylolytic fungus from 15 successful by isolates from sago industrial waste were selected and studied for crude enzyme starch digesting ability, enzyme properties, enzyme production as well as fungal morphological and molecular identification. This study had revealed that Ceratocystis par...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
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Universiti Malaysia Sarawak, UNIMAS
2007
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| Online Access: | http://ir.unimas.my/id/eprint/7620/ http://ir.unimas.my/id/eprint/7620/1/Koh.pdf http://ir.unimas.my/id/eprint/7620/4/Koh%20Seng%20Fook%20ft.pdf |
| _version_ | 1848836173131153408 |
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| author | Koh, Seng Fook |
| author_facet | Koh, Seng Fook |
| author_sort | Koh, Seng Fook |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Five best amylolytic fungus from 15 successful by isolates from sago industrial waste were selected and studied for crude enzyme starch digesting ability, enzyme properties, enzyme production as well as fungal morphological and molecular identification. This study had revealed that Ceratocystis
paradoxa is the best amylolytic fungus from all the isolates. Starch-iodine assay was use to determine the enzyme activity. The kinetic constant (Km), maximum velocity (Vmax) and catalytic constant (kcat) was recorded at 1363.5 mg/ml, 144.9 U/ml, 8.17 mg/ml minutes-1 respectively for Ceratocystis paradoxa. The crude enzyme is thermostable on all assayed temperature where the optimum temperature is 60oC, and
maintained high relative activity (80 % and above) in pH 4.0 to 8.0 from studies performed, as well as remaining stable in the presence of metal ions (Cu2+, CO2+, Mn2+, Ca2+, Zn2+, Mg2+, Fe2+) studied. The genetic related study between the 5 isolates via ITS primers and DNA sequencing was also performed.
Further study should be emphasizing on the optimization of fungal crude enzyme production, solid state fermentation, cloning of the enzyme expression gene, as well as further molecular studies on the development of expressed sequence tag (EST) and random sequence tags (RSTs) in the future. |
| first_indexed | 2025-11-15T06:19:33Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-7620 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:19:33Z |
| publishDate | 2007 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-76202023-10-10T02:53:22Z http://ir.unimas.my/id/eprint/7620/ Screening and isolation of amylolytic fungus and crude enzyme characterization from Sago industrial waste Koh, Seng Fook S Agriculture (General) Five best amylolytic fungus from 15 successful by isolates from sago industrial waste were selected and studied for crude enzyme starch digesting ability, enzyme properties, enzyme production as well as fungal morphological and molecular identification. This study had revealed that Ceratocystis paradoxa is the best amylolytic fungus from all the isolates. Starch-iodine assay was use to determine the enzyme activity. The kinetic constant (Km), maximum velocity (Vmax) and catalytic constant (kcat) was recorded at 1363.5 mg/ml, 144.9 U/ml, 8.17 mg/ml minutes-1 respectively for Ceratocystis paradoxa. The crude enzyme is thermostable on all assayed temperature where the optimum temperature is 60oC, and maintained high relative activity (80 % and above) in pH 4.0 to 8.0 from studies performed, as well as remaining stable in the presence of metal ions (Cu2+, CO2+, Mn2+, Ca2+, Zn2+, Mg2+, Fe2+) studied. The genetic related study between the 5 isolates via ITS primers and DNA sequencing was also performed. Further study should be emphasizing on the optimization of fungal crude enzyme production, solid state fermentation, cloning of the enzyme expression gene, as well as further molecular studies on the development of expressed sequence tag (EST) and random sequence tags (RSTs) in the future. Universiti Malaysia Sarawak, UNIMAS 2007 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7620/1/Koh.pdf text en http://ir.unimas.my/id/eprint/7620/4/Koh%20Seng%20Fook%20ft.pdf Koh, Seng Fook (2007) Screening and isolation of amylolytic fungus and crude enzyme characterization from Sago industrial waste. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | S Agriculture (General) Koh, Seng Fook Screening and isolation of amylolytic fungus and crude enzyme characterization from Sago industrial waste |
| title | Screening and isolation of amylolytic fungus and crude enzyme characterization from Sago industrial waste |
| title_full | Screening and isolation of amylolytic fungus and crude enzyme characterization from Sago industrial waste |
| title_fullStr | Screening and isolation of amylolytic fungus and crude enzyme characterization from Sago industrial waste |
| title_full_unstemmed | Screening and isolation of amylolytic fungus and crude enzyme characterization from Sago industrial waste |
| title_short | Screening and isolation of amylolytic fungus and crude enzyme characterization from Sago industrial waste |
| title_sort | screening and isolation of amylolytic fungus and crude enzyme characterization from sago industrial waste |
| topic | S Agriculture (General) |
| url | http://ir.unimas.my/id/eprint/7620/ http://ir.unimas.my/id/eprint/7620/1/Koh.pdf http://ir.unimas.my/id/eprint/7620/4/Koh%20Seng%20Fook%20ft.pdf |