Isolation of high quality genomic dna from dryobnanalops beccarii dyer tissues rich in camphor
The isolation of high-quality intact genomic DNA is obviously difficult in a variety of plant species due to the presence of high levels of polysaccharides, polyphenolics and other secondary metabolites. These contaminants will co-precipitate with DNA during DNA isolation and purification processes,...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak (UNIMAS)
2007
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| Online Access: | http://ir.unimas.my/id/eprint/7609/ http://ir.unimas.my/id/eprint/7609/3/LIEW%20KIT%20SIONG.pdf |
| _version_ | 1848836170028417024 |
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| author | Liew, Kit Siong |
| author_facet | Liew, Kit Siong |
| author_sort | Liew, Kit Siong |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | The isolation of high-quality intact genomic DNA is obviously difficult in a variety of plant species due to the presence of high levels of polysaccharides, polyphenolics and other secondary metabolites. These contaminants will co-precipitate with DNA during DNA isolation and purification processes, and therefore, resulting in a brownish DNA pellet that is unsuitable for downstream applications. Most plant genomic DNA
isolation protocols for various plant tissues have been established; however these protocols are inefficient in yielding high-quality amplifiable genomic DNA especially
from camphor containing timber species, Drynobanalops beccarii Dyer. In this project, the total genomic DNA of Drynobanalops beccarii was successfully isolated using a re-optimized CTAB method based on the Murray & Thompson (1980) and Doyle & Doyle (1990). The modifications include: 1) using 1% =-mercaptoethanol and 2% PVP (Mr 40000) in the extraction buffer, 2) sample incubation time, 40 minutes at 65°C; and 3) DNA precipitation at room temperature (25°C). The isolated
DNA pellet was in transparent colour and yielded amplifiable genomic DNA for RAPD-PCR. |
| first_indexed | 2025-11-15T06:19:30Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-7609 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:19:30Z |
| publishDate | 2007 |
| publisher | Universiti Malaysia Sarawak (UNIMAS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-76092024-01-02T07:33:30Z http://ir.unimas.my/id/eprint/7609/ Isolation of high quality genomic dna from dryobnanalops beccarii dyer tissues rich in camphor Liew, Kit Siong SB Plant culture The isolation of high-quality intact genomic DNA is obviously difficult in a variety of plant species due to the presence of high levels of polysaccharides, polyphenolics and other secondary metabolites. These contaminants will co-precipitate with DNA during DNA isolation and purification processes, and therefore, resulting in a brownish DNA pellet that is unsuitable for downstream applications. Most plant genomic DNA isolation protocols for various plant tissues have been established; however these protocols are inefficient in yielding high-quality amplifiable genomic DNA especially from camphor containing timber species, Drynobanalops beccarii Dyer. In this project, the total genomic DNA of Drynobanalops beccarii was successfully isolated using a re-optimized CTAB method based on the Murray & Thompson (1980) and Doyle & Doyle (1990). The modifications include: 1) using 1% =-mercaptoethanol and 2% PVP (Mr 40000) in the extraction buffer, 2) sample incubation time, 40 minutes at 65°C; and 3) DNA precipitation at room temperature (25°C). The isolated DNA pellet was in transparent colour and yielded amplifiable genomic DNA for RAPD-PCR. Universiti Malaysia Sarawak (UNIMAS) 2007 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7609/3/LIEW%20KIT%20SIONG.pdf Liew, Kit Siong (2007) Isolation of high quality genomic dna from dryobnanalops beccarii dyer tissues rich in camphor. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | SB Plant culture Liew, Kit Siong Isolation of high quality genomic dna from dryobnanalops beccarii dyer tissues rich in camphor |
| title | Isolation of high quality genomic dna from dryobnanalops beccarii dyer tissues rich in camphor |
| title_full | Isolation of high quality genomic dna from dryobnanalops beccarii dyer tissues rich in camphor |
| title_fullStr | Isolation of high quality genomic dna from dryobnanalops beccarii dyer tissues rich in camphor |
| title_full_unstemmed | Isolation of high quality genomic dna from dryobnanalops beccarii dyer tissues rich in camphor |
| title_short | Isolation of high quality genomic dna from dryobnanalops beccarii dyer tissues rich in camphor |
| title_sort | isolation of high quality genomic dna from dryobnanalops beccarii dyer tissues rich in camphor |
| topic | SB Plant culture |
| url | http://ir.unimas.my/id/eprint/7609/ http://ir.unimas.my/id/eprint/7609/3/LIEW%20KIT%20SIONG.pdf |