Suspension culture of Aquilaria beccariana van teigh
Aquilaria beccariana van Teigh (Thymelaceae), is a non timber forest product. The tree is valuable for its fragrant wood. Agarwood which is important for incense, traditional medicine, perfumery and others products. The population of Aquilaria has been reduced drastically due to the indiscriminat...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, UNIMAS
2013
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/7592/ http://ir.unimas.my/id/eprint/7592/8/Suspension%20culture%20of%20Aquilaria%20beccariana%20van%20teigh%20%28full%20text%29.pdf |
| _version_ | 1848836167745667072 |
|---|---|
| author | Khairunisa, Binti Ab Aziz |
| author_facet | Khairunisa, Binti Ab Aziz |
| author_sort | Khairunisa, Binti Ab Aziz |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Aquilaria beccariana van Teigh (Thymelaceae), is a non timber forest product. The tree is valuable
for its fragrant wood. Agarwood which is important for incense, traditional medicine, perfumery
and others products. The population of Aquilaria has been reduced drastically due to the
indiscriminate collection, over exploitation and lacking of rapid natural regeneration. The
population of this tree became declined and the production of gaharu is less compared to highly
demand of the user. Thus, in vitro technique was selected because it offer rapid regeneration and
conservation of these endangered species and this study, attempts were done to produce callus from
Aquilaria sp. Works were done to induce callus from lamina explants on MS solid and MS liquid
medium supplemented with different combination of hormone (2,4-D, picloram and NAA) and
varied concentration of sucrose (20-50 g/L). The callus culture was successfully established in MS
solid media supplemented with combination of 2.0 mg/L 2,4-D plus 0.5 mg/L BAP. While in MS
liquid medium, the suspension culture successfully established in combination of 3 mg/L NAA plus
0.5 mg/L BAP by obtaining highest frequency of fresh weight (2.09g). Thus, 40 g /L found to be
the best sucrose concentration with (1.95 g ) of fresh weight. While, combination of 0.25 mg/L
picloram with 2.0 mg/L 2,4-D and 0.5 mg/L BAP gave high frequency of fresh weight ( 1.06 g) in
Picloram effect. Suspension culture of callus gave friable and compact in texture and globular and
irregular in shape. |
| first_indexed | 2025-11-15T06:19:28Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-7592 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:19:28Z |
| publishDate | 2013 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-75922023-08-10T08:27:47Z http://ir.unimas.my/id/eprint/7592/ Suspension culture of Aquilaria beccariana van teigh Khairunisa, Binti Ab Aziz Q Science (General) QD Chemistry SB Plant culture Aquilaria beccariana van Teigh (Thymelaceae), is a non timber forest product. The tree is valuable for its fragrant wood. Agarwood which is important for incense, traditional medicine, perfumery and others products. The population of Aquilaria has been reduced drastically due to the indiscriminate collection, over exploitation and lacking of rapid natural regeneration. The population of this tree became declined and the production of gaharu is less compared to highly demand of the user. Thus, in vitro technique was selected because it offer rapid regeneration and conservation of these endangered species and this study, attempts were done to produce callus from Aquilaria sp. Works were done to induce callus from lamina explants on MS solid and MS liquid medium supplemented with different combination of hormone (2,4-D, picloram and NAA) and varied concentration of sucrose (20-50 g/L). The callus culture was successfully established in MS solid media supplemented with combination of 2.0 mg/L 2,4-D plus 0.5 mg/L BAP. While in MS liquid medium, the suspension culture successfully established in combination of 3 mg/L NAA plus 0.5 mg/L BAP by obtaining highest frequency of fresh weight (2.09g). Thus, 40 g /L found to be the best sucrose concentration with (1.95 g ) of fresh weight. While, combination of 0.25 mg/L picloram with 2.0 mg/L 2,4-D and 0.5 mg/L BAP gave high frequency of fresh weight ( 1.06 g) in Picloram effect. Suspension culture of callus gave friable and compact in texture and globular and irregular in shape. Universiti Malaysia Sarawak, UNIMAS 2013 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7592/8/Suspension%20culture%20of%20Aquilaria%20beccariana%20van%20teigh%20%28full%20text%29.pdf Khairunisa, Binti Ab Aziz (2013) Suspension culture of Aquilaria beccariana van teigh. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) QD Chemistry SB Plant culture Khairunisa, Binti Ab Aziz Suspension culture of Aquilaria beccariana van teigh |
| title | Suspension culture of Aquilaria beccariana van teigh |
| title_full | Suspension culture of Aquilaria beccariana van teigh |
| title_fullStr | Suspension culture of Aquilaria beccariana van teigh |
| title_full_unstemmed | Suspension culture of Aquilaria beccariana van teigh |
| title_short | Suspension culture of Aquilaria beccariana van teigh |
| title_sort | suspension culture of aquilaria beccariana van teigh |
| topic | Q Science (General) QD Chemistry SB Plant culture |
| url | http://ir.unimas.my/id/eprint/7592/ http://ir.unimas.my/id/eprint/7592/8/Suspension%20culture%20of%20Aquilaria%20beccariana%20van%20teigh%20%28full%20text%29.pdf |