Random amplified polymorphic DNA (RAPD) profiling of planted sago palm
Sago palm (Metroxylon sagu) is one of the commercially important palm species in Sarawak. They can occupy a diversity of habitats at different altitudes but they show dominancy for wet habitats. Sago palm is widely planted in Sarawak due to its commercial value. The objective of study is to isola...
| Main Author: | |
|---|---|
| Format: | Final Year Project Report / IMRAD |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak, UNIMAS
2013
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/7579/ http://ir.unimas.my/id/eprint/7579/1/Random%20amplified%20polymorphic%20DNA%20%28RAPD%29%20profiling%20of%20planted%20sago%20palm%20%2824pgs%29.pdf http://ir.unimas.my/id/eprint/7579/8/Allysya%20Sylvinessa%20Anak%20Alvin%20ft.pdf |
| Summary: | Sago palm (Metroxylon sagu) is one of the commercially important palm species
in Sarawak. They can occupy a diversity of habitats at different altitudes but they show
dominancy for wet habitats. Sago palm is widely planted in Sarawak due to its commercial
value. The objective of study is to isolate genomic DNA from planted sago palm and to
obtain DNA band profiles of the sago palm using the RAPD method. The DNA extraction
of the sago palm was conducted using common CTAB extraction method. This CTAB
method for DNA extraction of fresh tissue has been successfully used in laboratory for
wide variety of plant families. RAPD-PCR is a method which randomly amplifies genomic
DNA utilising single, short and arbitrary oligonucleotide primers. It is a fast and easy to
perform since no prior knowledge on the target sequence is needed. The DNA band
profiles were observed via agarose gel electrophoresis analysis. In this study, a good
quality and yield of sago palm genomic DNA was extracted. Also, DNA band profiles for
sago palm were successfully generated using RAPD commercial decamer primers. |
|---|