Molecular cloning and characterization of partial cDNA encoding for alpha-amylase inhibitor from kelampayan (neolamarckia cadamba)
Neolamarckia cadamba or Kelampayan is a type of deciduous tree species classified under the Rubiaceae family. The fast fast-growing N. cadamba emerges as a new alternative to meet high global demand of timber wood. Flavanone 3-hydroxylase is one of the key enzymes involved in the flavonoid synthe...
| Main Author: | |
|---|---|
| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, UNIMAS
2013
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/7551/ http://ir.unimas.my/id/eprint/7551/4/Li%20Fong%28full%20text%29.pdf |
| _version_ | 1848836157778952192 |
|---|---|
| author | Yong, Li Fong |
| author_facet | Yong, Li Fong |
| author_sort | Yong, Li Fong |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Neolamarckia cadamba or Kelampayan is a type of deciduous tree species classified
under the Rubiaceae family. The fast fast-growing N. cadamba emerges as a new
alternative to meet high global demand of timber wood. Flavanone 3-hydroxylase is one
of the key enzymes involved in the flavonoid synthesis pathway. Thus, this study aims to
amplify the flavanone 3-hydroxylase partial cDNA in order characterize it through
sequence analysis. High quality total RNA was extracted from the young leaves of N.
cadamba and converted into cDNA. Amplification of cDNA encoding flavanone 3-
hydroxylase was performed through PCR. The BLASTn result of purified PCR product
revealed high similarity with the flavanone 3-hydroxylase (F3H) mRNA of other plant
species, comfirming the identity of the nucleotide sequence as the partial cDNA of
flavanone 3-hydroxylase. |
| first_indexed | 2025-11-15T06:19:18Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-7551 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:19:18Z |
| publishDate | 2013 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-75512024-03-14T07:28:26Z http://ir.unimas.my/id/eprint/7551/ Molecular cloning and characterization of partial cDNA encoding for alpha-amylase inhibitor from kelampayan (neolamarckia cadamba) Yong, Li Fong QH301 Biology SB Plant culture Neolamarckia cadamba or Kelampayan is a type of deciduous tree species classified under the Rubiaceae family. The fast fast-growing N. cadamba emerges as a new alternative to meet high global demand of timber wood. Flavanone 3-hydroxylase is one of the key enzymes involved in the flavonoid synthesis pathway. Thus, this study aims to amplify the flavanone 3-hydroxylase partial cDNA in order characterize it through sequence analysis. High quality total RNA was extracted from the young leaves of N. cadamba and converted into cDNA. Amplification of cDNA encoding flavanone 3- hydroxylase was performed through PCR. The BLASTn result of purified PCR product revealed high similarity with the flavanone 3-hydroxylase (F3H) mRNA of other plant species, comfirming the identity of the nucleotide sequence as the partial cDNA of flavanone 3-hydroxylase. Universiti Malaysia Sarawak, UNIMAS 2013 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7551/4/Li%20Fong%28full%20text%29.pdf Yong, Li Fong (2013) Molecular cloning and characterization of partial cDNA encoding for alpha-amylase inhibitor from kelampayan (neolamarckia cadamba). [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | QH301 Biology SB Plant culture Yong, Li Fong Molecular cloning and characterization of partial cDNA encoding for alpha-amylase inhibitor from kelampayan (neolamarckia cadamba) |
| title | Molecular cloning and characterization of partial cDNA encoding for alpha-amylase inhibitor from kelampayan (neolamarckia cadamba) |
| title_full | Molecular cloning and characterization of partial cDNA encoding for alpha-amylase inhibitor from kelampayan (neolamarckia cadamba) |
| title_fullStr | Molecular cloning and characterization of partial cDNA encoding for alpha-amylase inhibitor from kelampayan (neolamarckia cadamba) |
| title_full_unstemmed | Molecular cloning and characterization of partial cDNA encoding for alpha-amylase inhibitor from kelampayan (neolamarckia cadamba) |
| title_short | Molecular cloning and characterization of partial cDNA encoding for alpha-amylase inhibitor from kelampayan (neolamarckia cadamba) |
| title_sort | molecular cloning and characterization of partial cdna encoding for alpha-amylase inhibitor from kelampayan (neolamarckia cadamba) |
| topic | QH301 Biology SB Plant culture |
| url | http://ir.unimas.my/id/eprint/7551/ http://ir.unimas.my/id/eprint/7551/4/Li%20Fong%28full%20text%29.pdf |