Genetic relatedness study of jatropha curcas genotypes using rapd molecular markers

Jatropha curcas is widely known as a source of high quality biodiesel. Due to the high demand of research on J. curcas accession, study of genetic relatedness of J. curcas accession from Sarawak (Lundu) to the other widely research J. curcas accession (Africa, Thailand and Indonesia) were conducte...

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Main Author: Nur Syuhadah, Mohd Gusti
Format: Final Year Project Report / IMRAD
Language:English
Published: Universiti Malaysia Sarawak, UNIMAS 2013
Subjects:
Online Access:http://ir.unimas.my/id/eprint/7534/
http://ir.unimas.my/id/eprint/7534/8/NUR%20SYUHADAH.pdf
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author Nur Syuhadah, Mohd Gusti
author_facet Nur Syuhadah, Mohd Gusti
author_sort Nur Syuhadah, Mohd Gusti
building UNIMAS Institutional Repository
collection Online Access
description Jatropha curcas is widely known as a source of high quality biodiesel. Due to the high demand of research on J. curcas accession, study of genetic relatedness of J. curcas accession from Sarawak (Lundu) to the other widely research J. curcas accession (Africa, Thailand and Indonesia) were conducted. The aim of this study was to assess the genetic relatedness of four different breeds of J. curcas (Africa, Thailand, Lundu (Sarawak, Malaysia) and Indonesia) by using RAPD marker. 10-mers primers were used to assess the genetic relatedness among the breeds. Jaccard formulation was used to calculate the similarity matrix. The RAPD-PCR analysis obtained shows high similarity matrix between Thailand and Indonesia (0.67) showing that both breeds come from a narrow genetic ancestor. Low similarity matrix of J. curcas of breed from Africa and Lundu to the other accession suggests that the breeds were not closely related. The successful revelation of genetic relatedness among the breeds indicates the effectiveness of RAPD profiling in the study. The outcomes of the study and the breeds are a good source for the development of excellent c J. curcas cultivars.
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format Final Year Project Report / IMRAD
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institution Universiti Malaysia Sarawak
institution_category Local University
language English
last_indexed 2025-11-15T06:19:14Z
publishDate 2013
publisher Universiti Malaysia Sarawak, UNIMAS
recordtype eprints
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spelling unimas-75342023-11-13T07:01:14Z http://ir.unimas.my/id/eprint/7534/ Genetic relatedness study of jatropha curcas genotypes using rapd molecular markers Nur Syuhadah, Mohd Gusti QH301 Biology QH426 Genetics Jatropha curcas is widely known as a source of high quality biodiesel. Due to the high demand of research on J. curcas accession, study of genetic relatedness of J. curcas accession from Sarawak (Lundu) to the other widely research J. curcas accession (Africa, Thailand and Indonesia) were conducted. The aim of this study was to assess the genetic relatedness of four different breeds of J. curcas (Africa, Thailand, Lundu (Sarawak, Malaysia) and Indonesia) by using RAPD marker. 10-mers primers were used to assess the genetic relatedness among the breeds. Jaccard formulation was used to calculate the similarity matrix. The RAPD-PCR analysis obtained shows high similarity matrix between Thailand and Indonesia (0.67) showing that both breeds come from a narrow genetic ancestor. Low similarity matrix of J. curcas of breed from Africa and Lundu to the other accession suggests that the breeds were not closely related. The successful revelation of genetic relatedness among the breeds indicates the effectiveness of RAPD profiling in the study. The outcomes of the study and the breeds are a good source for the development of excellent c J. curcas cultivars. Universiti Malaysia Sarawak, UNIMAS 2013 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7534/8/NUR%20SYUHADAH.pdf Nur Syuhadah, Mohd Gusti (2013) Genetic relatedness study of jatropha curcas genotypes using rapd molecular markers. [Final Year Project Report / IMRAD] (Unpublished)
spellingShingle QH301 Biology
QH426 Genetics
Nur Syuhadah, Mohd Gusti
Genetic relatedness study of jatropha curcas genotypes using rapd molecular markers
title Genetic relatedness study of jatropha curcas genotypes using rapd molecular markers
title_full Genetic relatedness study of jatropha curcas genotypes using rapd molecular markers
title_fullStr Genetic relatedness study of jatropha curcas genotypes using rapd molecular markers
title_full_unstemmed Genetic relatedness study of jatropha curcas genotypes using rapd molecular markers
title_short Genetic relatedness study of jatropha curcas genotypes using rapd molecular markers
title_sort genetic relatedness study of jatropha curcas genotypes using rapd molecular markers
topic QH301 Biology
QH426 Genetics
url http://ir.unimas.my/id/eprint/7534/
http://ir.unimas.my/id/eprint/7534/8/NUR%20SYUHADAH.pdf