Genetic relatedness study of jatropha curcas genotypes using rapd molecular markers
Jatropha curcas is widely known as a source of high quality biodiesel. Due to the high demand of research on J. curcas accession, study of genetic relatedness of J. curcas accession from Sarawak (Lundu) to the other widely research J. curcas accession (Africa, Thailand and Indonesia) were conducte...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, UNIMAS
2013
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| Online Access: | http://ir.unimas.my/id/eprint/7534/ http://ir.unimas.my/id/eprint/7534/8/NUR%20SYUHADAH.pdf |
| Summary: | Jatropha curcas is widely known as a source of high quality biodiesel. Due to the high demand of research on J.
curcas accession, study of genetic relatedness of J. curcas accession from Sarawak (Lundu) to the other widely
research J. curcas accession (Africa, Thailand and Indonesia) were conducted. The aim of this study was to
assess the genetic relatedness of four different breeds of J. curcas (Africa, Thailand, Lundu (Sarawak, Malaysia)
and Indonesia) by using RAPD marker. 10-mers primers were used to assess the genetic relatedness among the
breeds. Jaccard formulation was used to calculate the similarity matrix. The RAPD-PCR analysis obtained shows
high similarity matrix between Thailand and Indonesia (0.67) showing that both breeds come from a narrow
genetic ancestor. Low similarity matrix of J. curcas of breed from Africa and Lundu to the other accession
suggests that the breeds were not closely related. The successful revelation of genetic relatedness among the
breeds indicates the effectiveness of RAPD profiling in the study. The outcomes of the study and the breeds are a
good source for the development of excellent c J. curcas cultivars. |
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