Evolution of Breeding mode in Bornean frogs
Bornean frogs used different types of lifestyles as they choose suitable places to breed in order to fulfill the needs of their young. Thus, they have different types of breeding modes which related to their specific microhabitat. Mitochondrial DNA of partial 16S rRNA gene was used in inferring th...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, UNIMAS
2013
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| Online Access: | http://ir.unimas.my/id/eprint/7531/ http://ir.unimas.my/id/eprint/7531/3/Evolution%20of%20Breeding%20Mode%20in%20Bornean%20Frogs%28full%20text%29.pdf |
| Summary: | Bornean frogs used different types of lifestyles as they choose suitable places to breed in order to fulfill the
needs of their young. Thus, they have different types of breeding modes which related to their specific
microhabitat. Mitochondrial DNA of partial 16S rRNA gene was used in inferring the genetic study of
Bornean frogs’ families. The study was aimed to determine the evolution of breeding mode in families of
Bornean frogs based on phylogenetic and breeding mode study. A total of 526 bp of 16S rRNA gene from 24
samples were analyzed using molecular technique approach (PCR-direct sequencing). Character coding based
on previous study data was used in constructing trees by using the Neighbor-Joining (NJ), and Maximum
parsimony (MP). The phylogenetic trees topologies based on 16S rRNA gene show no pattern of each
relationship of the breeding mode of frogs’ family. Although partial gene 16S rRNA was used to study
amphibian at a specific level, however 16S rRNA is not a suitable gene marker to highlights the breeding
mode. Besides that, the tree produced from character coding based on previous study does not have enough
characters to infer the representative species. This study should be enhanced in future in order to infer the
phylogeny of Bornean frogs based on DNA and breeding mode by adding samples, characters, and breeding
mode. |
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