Comparative Analysis of expression patterns among the ribosomal protein genes of rpl27, rpl37a, and rpl41 in human cell line
Ribosomal proteins are essential in protein biosynthesis process and they also have extraribosomal functions. These proteins have been found to be closely associated with various inherited diseases and cancers. Previous studies revealed that the ribosomal protein large subunit (RPL) gene expressed...
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| Format: | Final Year Project Report / IMRAD |
| Language: | English |
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Universiti Malaysia Sarawak, UNIMAS
2013
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| Online Access: | http://ir.unimas.my/id/eprint/7487/ http://ir.unimas.my/id/eprint/7487/8/Comparative%20analysis%20of%20expression%20patterns%20among%20the%20ribosomal%20protein%20genes%20of%20RPL27%20%28OCR%29.pdf |
| _version_ | 1848836142973059072 |
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| author | Chin, Fiona Sze Yen |
| author_facet | Chin, Fiona Sze Yen |
| author_sort | Chin, Fiona Sze Yen |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Ribosomal proteins are essential in protein biosynthesis process and they also have extraribosomal functions.
These proteins have been found to be closely associated with various inherited diseases and cancers. Previous
studies revealed that the ribosomal protein large subunit (RPL) gene expressed differently among cell line
derived from human nasopharyngeal carcinoma (NPC). However, RPL27, RPL37a, and RPL41 are found to
be significantly downregulated in all cell lines derived from NPC tumours when compared to normal control.
The main objective of this study is to evaluate quantitatively the expressed transcript level among the RP genes of RPL27, RPL37a, and RPL41 in the human cell line system. The method of Reverse Transcription
Polymerase Chain Reaction (RT-PCR) coupled with band intensity analysis will be used to meet this
objective. Result from this finding show that there are no significant different in expressions of RPL27,
RPL37a, and RPL41 in NPC tumours and normal control. Besides that, RPL27 and RPL37a show significant
correlation when increase in sample size. |
| first_indexed | 2025-11-15T06:19:04Z |
| format | Final Year Project Report / IMRAD |
| id | unimas-7487 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:19:04Z |
| publishDate | 2013 |
| publisher | Universiti Malaysia Sarawak, UNIMAS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-74872023-01-31T03:44:21Z http://ir.unimas.my/id/eprint/7487/ Comparative Analysis of expression patterns among the ribosomal protein genes of rpl27, rpl37a, and rpl41 in human cell line Chin, Fiona Sze Yen Q Science (General) QH301 Biology Ribosomal proteins are essential in protein biosynthesis process and they also have extraribosomal functions. These proteins have been found to be closely associated with various inherited diseases and cancers. Previous studies revealed that the ribosomal protein large subunit (RPL) gene expressed differently among cell line derived from human nasopharyngeal carcinoma (NPC). However, RPL27, RPL37a, and RPL41 are found to be significantly downregulated in all cell lines derived from NPC tumours when compared to normal control. The main objective of this study is to evaluate quantitatively the expressed transcript level among the RP genes of RPL27, RPL37a, and RPL41 in the human cell line system. The method of Reverse Transcription Polymerase Chain Reaction (RT-PCR) coupled with band intensity analysis will be used to meet this objective. Result from this finding show that there are no significant different in expressions of RPL27, RPL37a, and RPL41 in NPC tumours and normal control. Besides that, RPL27 and RPL37a show significant correlation when increase in sample size. Universiti Malaysia Sarawak, UNIMAS 2013 Final Year Project Report / IMRAD NonPeerReviewed text en http://ir.unimas.my/id/eprint/7487/8/Comparative%20analysis%20of%20expression%20patterns%20among%20the%20ribosomal%20protein%20genes%20of%20RPL27%20%28OCR%29.pdf Chin, Fiona Sze Yen (2013) Comparative Analysis of expression patterns among the ribosomal protein genes of rpl27, rpl37a, and rpl41 in human cell line. [Final Year Project Report / IMRAD] (Unpublished) |
| spellingShingle | Q Science (General) QH301 Biology Chin, Fiona Sze Yen Comparative Analysis of expression patterns among the ribosomal protein genes of rpl27, rpl37a, and rpl41 in human cell line |
| title | Comparative Analysis of expression patterns among the ribosomal protein genes of rpl27, rpl37a, and rpl41 in human cell line |
| title_full | Comparative Analysis of expression patterns among the ribosomal protein genes of rpl27, rpl37a, and rpl41 in human cell line |
| title_fullStr | Comparative Analysis of expression patterns among the ribosomal protein genes of rpl27, rpl37a, and rpl41 in human cell line |
| title_full_unstemmed | Comparative Analysis of expression patterns among the ribosomal protein genes of rpl27, rpl37a, and rpl41 in human cell line |
| title_short | Comparative Analysis of expression patterns among the ribosomal protein genes of rpl27, rpl37a, and rpl41 in human cell line |
| title_sort | comparative analysis of expression patterns among the ribosomal protein genes of rpl27, rpl37a, and rpl41 in human cell line |
| topic | Q Science (General) QH301 Biology |
| url | http://ir.unimas.my/id/eprint/7487/ http://ir.unimas.my/id/eprint/7487/8/Comparative%20analysis%20of%20expression%20patterns%20among%20the%20ribosomal%20protein%20genes%20of%20RPL27%20%28OCR%29.pdf |