The Role of Polymerase Chain Reaction (PCR) in Diagnosis of Spine Tuberculosis after Pre-operative Anti-tuberculosis Treatment
Objective: The aim of this study was to evaluate the role of polymerase chain reaction (PCR) in the diagnosis of spinal tuberculosis after 2 weeks of preoperative anti-tuberculosis treatment and to compare PCR to the Löwenstein - Jensen Culture (LJC) and histopathological examination (HPE) meth...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Malaysian Orthopaedic Association
2011
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/7458/ http://ir.unimas.my/id/eprint/7458/1/Polymerase_Chain_Reaction.pdf |
| _version_ | 1848836135299579904 |
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| author | Ahmad Hata, Rasit SF, Ibrahim CC, Wong |
| author_facet | Ahmad Hata, Rasit SF, Ibrahim CC, Wong |
| author_sort | Ahmad Hata, Rasit |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Objective:
The aim of this study was to evaluate the role of
polymerase chain reaction (PCR) in the diagnosis of spinal tuberculosis after 2 weeks of preoperative anti-tuberculosis treatment and to compare PCR to the Löwenstein - Jensen Culture (LJC) and histopathological examination (HPE) methods.
Methods:
Twenty-five patients were included in this study. Sixteen patients were diagnosed and treated for spinal tuberculosis based on clinical and radiological evidence. Nine patients were controls. The LJC method and HPE of the specimen were performed according to hospital protocol. PCR was performed using primer encoding insertion of sequences IS6110 for mycobacterium tuberculosis complex. Clinical findings and radiological features were the gold standard for comparison.
Results:
PCR results were 15 positive and one negative. The
sensitivity and specificity of PCR was 94% and 100%
respectively (with 95% confidence interval [CI] 67% to 99% and 63% to 100%, respectively). HPE results showed 13
were positive and 3 negative in the spinal tuberculosis group; for the control group, all were negative. Sensitivity and specificity value of HPE was 82 % and 100% respectively (with 95% confidence interval [CI] 54% to 95% and 63% to 100%, respectively). Use of LJC showed only one was positive and 15 were negative in the spinal tuberculosis group whole all nine in the control group were negative. Sensitivity and specificity value of LJC was 6% and 100% respectively (with 95% confidence interval [CI] 0.3% to 32% and 63% to 100%, respectively).
Conclusion:
Our findings showed that the PCR for Mycobacterium
tuberculosis is reliable as a method for diagnosis of spinal tuberculosis, even after of 2 weeks of anti-TB treatment, with an overall sensitivity of 94% and specificity of 100%. |
| first_indexed | 2025-11-15T06:18:57Z |
| format | Article |
| id | unimas-7458 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:18:57Z |
| publishDate | 2011 |
| publisher | Malaysian Orthopaedic Association |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-74582017-03-02T02:43:31Z http://ir.unimas.my/id/eprint/7458/ The Role of Polymerase Chain Reaction (PCR) in Diagnosis of Spine Tuberculosis after Pre-operative Anti-tuberculosis Treatment Ahmad Hata, Rasit SF, Ibrahim CC, Wong QR Microbiology RD Surgery RZ Other systems of medicine Objective: The aim of this study was to evaluate the role of polymerase chain reaction (PCR) in the diagnosis of spinal tuberculosis after 2 weeks of preoperative anti-tuberculosis treatment and to compare PCR to the Löwenstein - Jensen Culture (LJC) and histopathological examination (HPE) methods. Methods: Twenty-five patients were included in this study. Sixteen patients were diagnosed and treated for spinal tuberculosis based on clinical and radiological evidence. Nine patients were controls. The LJC method and HPE of the specimen were performed according to hospital protocol. PCR was performed using primer encoding insertion of sequences IS6110 for mycobacterium tuberculosis complex. Clinical findings and radiological features were the gold standard for comparison. Results: PCR results were 15 positive and one negative. The sensitivity and specificity of PCR was 94% and 100% respectively (with 95% confidence interval [CI] 67% to 99% and 63% to 100%, respectively). HPE results showed 13 were positive and 3 negative in the spinal tuberculosis group; for the control group, all were negative. Sensitivity and specificity value of HPE was 82 % and 100% respectively (with 95% confidence interval [CI] 54% to 95% and 63% to 100%, respectively). Use of LJC showed only one was positive and 15 were negative in the spinal tuberculosis group whole all nine in the control group were negative. Sensitivity and specificity value of LJC was 6% and 100% respectively (with 95% confidence interval [CI] 0.3% to 32% and 63% to 100%, respectively). Conclusion: Our findings showed that the PCR for Mycobacterium tuberculosis is reliable as a method for diagnosis of spinal tuberculosis, even after of 2 weeks of anti-TB treatment, with an overall sensitivity of 94% and specificity of 100%. Malaysian Orthopaedic Association 2011-03-01 Article PeerReviewed text en http://ir.unimas.my/id/eprint/7458/1/Polymerase_Chain_Reaction.pdf Ahmad Hata, Rasit and SF, Ibrahim and CC, Wong (2011) The Role of Polymerase Chain Reaction (PCR) in Diagnosis of Spine Tuberculosis after Pre-operative Anti-tuberculosis Treatment. Malaysia Orthopaedic Journal, 5 (1). pp. 8-12. ISSN 1985-2533 http://www.morthoj.org/2011v5n1/Polymerase_Chain_Reaction.pdf doi: 10.5704/MOJ.1103.002 |
| spellingShingle | QR Microbiology RD Surgery RZ Other systems of medicine Ahmad Hata, Rasit SF, Ibrahim CC, Wong The Role of Polymerase Chain Reaction (PCR) in Diagnosis of Spine Tuberculosis after Pre-operative Anti-tuberculosis Treatment |
| title | The Role of Polymerase Chain Reaction (PCR) in Diagnosis
of Spine Tuberculosis after Pre-operative Anti-tuberculosis
Treatment |
| title_full | The Role of Polymerase Chain Reaction (PCR) in Diagnosis
of Spine Tuberculosis after Pre-operative Anti-tuberculosis
Treatment |
| title_fullStr | The Role of Polymerase Chain Reaction (PCR) in Diagnosis
of Spine Tuberculosis after Pre-operative Anti-tuberculosis
Treatment |
| title_full_unstemmed | The Role of Polymerase Chain Reaction (PCR) in Diagnosis
of Spine Tuberculosis after Pre-operative Anti-tuberculosis
Treatment |
| title_short | The Role of Polymerase Chain Reaction (PCR) in Diagnosis
of Spine Tuberculosis after Pre-operative Anti-tuberculosis
Treatment |
| title_sort | role of polymerase chain reaction (pcr) in diagnosis
of spine tuberculosis after pre-operative anti-tuberculosis
treatment |
| topic | QR Microbiology RD Surgery RZ Other systems of medicine |
| url | http://ir.unimas.my/id/eprint/7458/ http://ir.unimas.my/id/eprint/7458/ http://ir.unimas.my/id/eprint/7458/ http://ir.unimas.my/id/eprint/7458/1/Polymerase_Chain_Reaction.pdf |