Genomic Diversity of Cholera Outbreak Strains in East Malaysia
Thirty one Vibrio cholera isolates recovered from cholera outbreak in Bintulu, Sarawak (Malaysia) were detected with the presence of ctx gene by using specific PCR. These isolates were further characterized and differentiated by using the Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR...
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| Format: | Article |
| Language: | English English |
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Universiti Putra Malaysia
2014
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| Online Access: | http://ir.unimas.my/id/eprint/7389/ http://ir.unimas.my/id/eprint/7389/1/Genomic%20Diversity%20of%20Cholera%20Outbreak%20Strains%20in%20East%20Malaysia%20%28abstract%29.pdf http://ir.unimas.my/id/eprint/7389/2/Genomic%20Diversity%20of%20Cholera%20Outbreak%20Strains%20in%20East%20Malaysia%20%28OCR%29.pdf |
| _version_ | 1848836117811429376 |
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| author | Lesley Maurice, Bilung Fuh, Yong Sy Velnetti, Linang Adom, Benjamin Micky, Vincent Kasing, Apun Samuel, Lihan Lin, Chien Su |
| author_facet | Lesley Maurice, Bilung Fuh, Yong Sy Velnetti, Linang Adom, Benjamin Micky, Vincent Kasing, Apun Samuel, Lihan Lin, Chien Su |
| author_sort | Lesley Maurice, Bilung |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Thirty one Vibrio cholera isolates recovered from cholera outbreak in Bintulu, Sarawak (Malaysia) were detected with the presence of ctx gene by using specific PCR. These isolates were further characterized and differentiated by using the Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) and BOX-PCR to determine their genomic fingerprints. The specific PCR result confirmed the identities of 27 isolates out of 31 as pathogenic V. cholerae. The ERIC-PCR generated several genetic profiles consisting of 4-6 bands with sizes in the range of 100 to 600 bp, while the BOX-PCR produced profiles numbering 2-7 bands in the sizes between 200 to 1000 bp. Based on the dendrogram generated from the
DNA fingerprinting profiles (ERIC-PCR and BOX-PCR), all of the isolates can be divided into 2 main clusters that is further divided into 2 sub-clusters. The low genetic diversity of the isolates indicated the outbreak of V. cholerae in the study area was due to the contamination from a single or few sources of V. cholerae. |
| first_indexed | 2025-11-15T06:18:40Z |
| format | Article |
| id | unimas-7389 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T06:18:40Z |
| publishDate | 2014 |
| publisher | Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-73892016-04-14T01:19:14Z http://ir.unimas.my/id/eprint/7389/ Genomic Diversity of Cholera Outbreak Strains in East Malaysia Lesley Maurice, Bilung Fuh, Yong Sy Velnetti, Linang Adom, Benjamin Micky, Vincent Kasing, Apun Samuel, Lihan Lin, Chien Su GE Environmental Sciences Q Science (General) QR Microbiology Thirty one Vibrio cholera isolates recovered from cholera outbreak in Bintulu, Sarawak (Malaysia) were detected with the presence of ctx gene by using specific PCR. These isolates were further characterized and differentiated by using the Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) and BOX-PCR to determine their genomic fingerprints. The specific PCR result confirmed the identities of 27 isolates out of 31 as pathogenic V. cholerae. The ERIC-PCR generated several genetic profiles consisting of 4-6 bands with sizes in the range of 100 to 600 bp, while the BOX-PCR produced profiles numbering 2-7 bands in the sizes between 200 to 1000 bp. Based on the dendrogram generated from the DNA fingerprinting profiles (ERIC-PCR and BOX-PCR), all of the isolates can be divided into 2 main clusters that is further divided into 2 sub-clusters. The low genetic diversity of the isolates indicated the outbreak of V. cholerae in the study area was due to the contamination from a single or few sources of V. cholerae. Universiti Putra Malaysia 2014 Article PeerReviewed text en http://ir.unimas.my/id/eprint/7389/1/Genomic%20Diversity%20of%20Cholera%20Outbreak%20Strains%20in%20East%20Malaysia%20%28abstract%29.pdf text en http://ir.unimas.my/id/eprint/7389/2/Genomic%20Diversity%20of%20Cholera%20Outbreak%20Strains%20in%20East%20Malaysia%20%28OCR%29.pdf Lesley Maurice, Bilung and Fuh, Yong Sy and Velnetti, Linang and Adom, Benjamin and Micky, Vincent and Kasing, Apun and Samuel, Lihan and Lin, Chien Su (2014) Genomic Diversity of Cholera Outbreak Strains in East Malaysia. Malaysian Journal of Medicine and Health Sciences, 10 (2). pp. 19-26. ISSN 1675-8544 http://t.upm.edu.my/dokumen/FKUSK1_Jurnal_Text_Article_3.pdf |
| spellingShingle | GE Environmental Sciences Q Science (General) QR Microbiology Lesley Maurice, Bilung Fuh, Yong Sy Velnetti, Linang Adom, Benjamin Micky, Vincent Kasing, Apun Samuel, Lihan Lin, Chien Su Genomic Diversity of Cholera Outbreak Strains in East Malaysia |
| title | Genomic Diversity of Cholera Outbreak Strains in East Malaysia |
| title_full | Genomic Diversity of Cholera Outbreak Strains in East Malaysia |
| title_fullStr | Genomic Diversity of Cholera Outbreak Strains in East Malaysia |
| title_full_unstemmed | Genomic Diversity of Cholera Outbreak Strains in East Malaysia |
| title_short | Genomic Diversity of Cholera Outbreak Strains in East Malaysia |
| title_sort | genomic diversity of cholera outbreak strains in east malaysia |
| topic | GE Environmental Sciences Q Science (General) QR Microbiology |
| url | http://ir.unimas.my/id/eprint/7389/ http://ir.unimas.my/id/eprint/7389/ http://ir.unimas.my/id/eprint/7389/1/Genomic%20Diversity%20of%20Cholera%20Outbreak%20Strains%20in%20East%20Malaysia%20%28abstract%29.pdf http://ir.unimas.my/id/eprint/7389/2/Genomic%20Diversity%20of%20Cholera%20Outbreak%20Strains%20in%20East%20Malaysia%20%28OCR%29.pdf |