Incidence of Vibrio parahaemolyticus at Estuarine Region of Sg. Sarawak (Sarawak) Influenced by Physicochemical Parameters

This study aimed to determine the correlation between physicochemical parameters namely salinity, pH and temperature with the occurrence of V. parahaemolyticus in estuarine river of Sg. Sarawak. A total of 72 water samples (surface and deep waters) were collected from the study site. A combination o...

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Main Author: Lesley Maurice, Bilung
Format: Article
Language:English
Published: Hibiscus Publisher 2014
Subjects:
Online Access:http://ir.unimas.my/id/eprint/7384/
http://ir.unimas.my/id/eprint/7384/1/Incidence%20of%20Vibrio%20-%20Copy.pdf
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author Lesley Maurice, Bilung
author_facet Lesley Maurice, Bilung
author_sort Lesley Maurice, Bilung
building UNIMAS Institutional Repository
collection Online Access
description This study aimed to determine the correlation between physicochemical parameters namely salinity, pH and temperature with the occurrence of V. parahaemolyticus in estuarine river of Sg. Sarawak. A total of 72 water samples (surface and deep waters) were collected from the study site. A combination of MPN and PCR to detect and quantify the presence of V. parahaemolyticus, targeting the regulatory gene, toxR was performed. Over the 12-weeks sampling period, site 2 and site 3 recorded 100% presence of V. parahaemolyticus while site I was only 17%. The conditions found in site 2 (9.75‰, pH 7.2, 29.65 °C) and 3 (10.3‰, pH 7.36, 30 °C) favored the presence of V. parahaemolyticus (669 cells 10 ml-1 and 922 cells 10 ml-1 respectively) than site 1 (0.68‰, pH 6.75, 29.2 °C). Statistical analysis showed that the abundance of total V. parahaemolyticus counts was correlated well with the water salinity (Pearson correlation coefficient, r = 0.699; P < 0.01) and water temperature (r = 0.531; P < 0.01) but less correlated with water pH (r = 0.343; P < 0.01).
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spelling unimas-73842022-08-16T07:31:22Z http://ir.unimas.my/id/eprint/7384/ Incidence of Vibrio parahaemolyticus at Estuarine Region of Sg. Sarawak (Sarawak) Influenced by Physicochemical Parameters Lesley Maurice, Bilung GE Environmental Sciences Q Science (General) This study aimed to determine the correlation between physicochemical parameters namely salinity, pH and temperature with the occurrence of V. parahaemolyticus in estuarine river of Sg. Sarawak. A total of 72 water samples (surface and deep waters) were collected from the study site. A combination of MPN and PCR to detect and quantify the presence of V. parahaemolyticus, targeting the regulatory gene, toxR was performed. Over the 12-weeks sampling period, site 2 and site 3 recorded 100% presence of V. parahaemolyticus while site I was only 17%. The conditions found in site 2 (9.75‰, pH 7.2, 29.65 °C) and 3 (10.3‰, pH 7.36, 30 °C) favored the presence of V. parahaemolyticus (669 cells 10 ml-1 and 922 cells 10 ml-1 respectively) than site 1 (0.68‰, pH 6.75, 29.2 °C). Statistical analysis showed that the abundance of total V. parahaemolyticus counts was correlated well with the water salinity (Pearson correlation coefficient, r = 0.699; P < 0.01) and water temperature (r = 0.531; P < 0.01) but less correlated with water pH (r = 0.343; P < 0.01). Hibiscus Publisher 2014 Article PeerReviewed text en http://ir.unimas.my/id/eprint/7384/1/Incidence%20of%20Vibrio%20-%20Copy.pdf Lesley Maurice, Bilung (2014) Incidence of Vibrio parahaemolyticus at Estuarine Region of Sg. Sarawak (Sarawak) Influenced by Physicochemical Parameters. Bioremediation Science And Technology Research, 2 (1). pp. 9-13. ISSN 2289-5892 http://www.researchgate.net/publication/266081163_Incidence_of_Vibrio_parahaemolyticus_at_Estuarine_Region_of_Sg._Sarawak_%28Sarawak%29_Influenced_by_Physicochemical_Parameters
spellingShingle GE Environmental Sciences
Q Science (General)
Lesley Maurice, Bilung
Incidence of Vibrio parahaemolyticus at Estuarine Region of Sg. Sarawak (Sarawak) Influenced by Physicochemical Parameters
title Incidence of Vibrio parahaemolyticus at Estuarine Region of Sg. Sarawak (Sarawak) Influenced by Physicochemical Parameters
title_full Incidence of Vibrio parahaemolyticus at Estuarine Region of Sg. Sarawak (Sarawak) Influenced by Physicochemical Parameters
title_fullStr Incidence of Vibrio parahaemolyticus at Estuarine Region of Sg. Sarawak (Sarawak) Influenced by Physicochemical Parameters
title_full_unstemmed Incidence of Vibrio parahaemolyticus at Estuarine Region of Sg. Sarawak (Sarawak) Influenced by Physicochemical Parameters
title_short Incidence of Vibrio parahaemolyticus at Estuarine Region of Sg. Sarawak (Sarawak) Influenced by Physicochemical Parameters
title_sort incidence of vibrio parahaemolyticus at estuarine region of sg. sarawak (sarawak) influenced by physicochemical parameters
topic GE Environmental Sciences
Q Science (General)
url http://ir.unimas.my/id/eprint/7384/
http://ir.unimas.my/id/eprint/7384/
http://ir.unimas.my/id/eprint/7384/1/Incidence%20of%20Vibrio%20-%20Copy.pdf