Identification of Methane-producing Bacteria from Palm Oil Mill Sludge (POMS) with Solid Cud from Ruminant Stomach
Biological generation in anaerobic environments such as enteric fermentation and anaerobic waste treatment from agriculture sector are the major contributor of methane gas which has the potential as biogas. The aimed of this study was to identify methane-producing bacteria in anaerobic vessel which...
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| Format: | Article |
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Hibiscus Publisher
2014
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| Online Access: | http://ir.unimas.my/id/eprint/7376/ |
| _version_ | 1848836114470666240 |
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| author | Lau, Seng Chan, CSW Husaini, A. A. S. A Zulkharnain, A. Kasing, Apun Bilung, L. M Micky, Vincent |
| author_facet | Lau, Seng Chan, CSW Husaini, A. A. S. A Zulkharnain, A. Kasing, Apun Bilung, L. M Micky, Vincent |
| author_sort | Lau, Seng |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Biological generation in anaerobic environments such as enteric fermentation and anaerobic waste treatment from agriculture sector are the major contributor of methane gas which has the
potential as biogas. The aimed of this study was to identify methane-producing bacteria in anaerobic vessel which contained a mixture of Palm Oil Mill Sludge (POMS) and solid cud
taken from the first compartment of cow’s stomach (1:2 and 2:1 ratio) as co-mixture. The comixture was incubated at 50 °C in a 2 L vessel with initial starter of 400 ml and sampling was
conducted every 4 weeks interval during 12 weeks of incubation. For specific detection of methanogens, 16S rRNA-cloning analysis was carried out. Methanobrevibacter sp. and
Methanosaeta sp. were confirmed to be presence within the 2:1 ratio of co-mixture while only Methanobrevibacter sp. was found in 1:2 ratio of co-mixture on both Week 0 and Week 4. No
methanogens were detected for both co-mixtures on Week 8 and Week 12. |
| first_indexed | 2025-11-15T06:18:37Z |
| format | Article |
| id | unimas-7376 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| last_indexed | 2025-11-15T06:18:37Z |
| publishDate | 2014 |
| publisher | Hibiscus Publisher |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-73762023-07-31T03:51:27Z http://ir.unimas.my/id/eprint/7376/ Identification of Methane-producing Bacteria from Palm Oil Mill Sludge (POMS) with Solid Cud from Ruminant Stomach Lau, Seng Chan, CSW Husaini, A. A. S. A Zulkharnain, A. Kasing, Apun Bilung, L. M Micky, Vincent GE Environmental Sciences QR Microbiology Biological generation in anaerobic environments such as enteric fermentation and anaerobic waste treatment from agriculture sector are the major contributor of methane gas which has the potential as biogas. The aimed of this study was to identify methane-producing bacteria in anaerobic vessel which contained a mixture of Palm Oil Mill Sludge (POMS) and solid cud taken from the first compartment of cow’s stomach (1:2 and 2:1 ratio) as co-mixture. The comixture was incubated at 50 °C in a 2 L vessel with initial starter of 400 ml and sampling was conducted every 4 weeks interval during 12 weeks of incubation. For specific detection of methanogens, 16S rRNA-cloning analysis was carried out. Methanobrevibacter sp. and Methanosaeta sp. were confirmed to be presence within the 2:1 ratio of co-mixture while only Methanobrevibacter sp. was found in 1:2 ratio of co-mixture on both Week 0 and Week 4. No methanogens were detected for both co-mixtures on Week 8 and Week 12. Hibiscus Publisher 2014 Article PeerReviewed Lau, Seng and Chan, CSW and Husaini, A. A. S. A and Zulkharnain, A. and Kasing, Apun and Bilung, L. M and Micky, Vincent (2014) Identification of Methane-producing Bacteria from Palm Oil Mill Sludge (POMS) with Solid Cud from Ruminant Stomach. Journal Of Biochemistry, Microbiology And Biotechnology, 1 (1). pp. 23-26. http://www.google.com.my/url?sa=t&rct=j&q=&esrc=s&source=web&cd=2&ved=0CCEQFjAB&url=http%3A%2F%2Fjournal.hibiscuspublisher.com%2Findex.php%2FJOBIMB%2Farticle%2Fdownload%2F125%2F185&ei=BQtQVdC_AdLIuASx34HADg&usg=AFQjCNH2FWdic1PX1H9KyRPV8HPUEtUiYg&cad=rja |
| spellingShingle | GE Environmental Sciences QR Microbiology Lau, Seng Chan, CSW Husaini, A. A. S. A Zulkharnain, A. Kasing, Apun Bilung, L. M Micky, Vincent Identification of Methane-producing Bacteria from Palm Oil Mill Sludge (POMS) with Solid Cud from Ruminant Stomach |
| title | Identification of Methane-producing Bacteria from Palm Oil Mill Sludge (POMS) with Solid Cud from Ruminant Stomach |
| title_full | Identification of Methane-producing Bacteria from Palm Oil Mill Sludge (POMS) with Solid Cud from Ruminant Stomach |
| title_fullStr | Identification of Methane-producing Bacteria from Palm Oil Mill Sludge (POMS) with Solid Cud from Ruminant Stomach |
| title_full_unstemmed | Identification of Methane-producing Bacteria from Palm Oil Mill Sludge (POMS) with Solid Cud from Ruminant Stomach |
| title_short | Identification of Methane-producing Bacteria from Palm Oil Mill Sludge (POMS) with Solid Cud from Ruminant Stomach |
| title_sort | identification of methane-producing bacteria from palm oil mill sludge (poms) with solid cud from ruminant stomach |
| topic | GE Environmental Sciences QR Microbiology |
| url | http://ir.unimas.my/id/eprint/7376/ http://ir.unimas.my/id/eprint/7376/ |