Risk modification of colorectal cancer susceptibility by interleukin-8 -251T>A polymorphism in Malaysians

AIM: To investigate the allele and genotype frequencies and associated risk of interleukin (IL )-8 -251T>A polymorphism on colorectal cancer (CRC) susceptibility risk. METHODS: Peripheral blood samples of 255 normal controls and 255 clinically and histopathologically confirmed CRC patients...

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Main Authors: Mohd Aminudin, Mustapha, Siti Nurfatimah, Mohd Shahpudin, Ahmad Aizat, Abdul Aziz, Ankathil, Ravindran
Format: Article
Language:English
Published: Baishideng 2012
Subjects:
Online Access:http://ir.unimas.my/id/eprint/734/
http://ir.unimas.my/id/eprint/734/1/Risk%20modification%20of%20colorectal%20cancer%28abstract%29.pdf
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author Mohd Aminudin, Mustapha
Siti Nurfatimah, Mohd Shahpudin
Ahmad Aizat, Abdul Aziz
Ankathil, Ravindran
author_facet Mohd Aminudin, Mustapha
Siti Nurfatimah, Mohd Shahpudin
Ahmad Aizat, Abdul Aziz
Ankathil, Ravindran
author_sort Mohd Aminudin, Mustapha
building UNIMAS Institutional Repository
collection Online Access
description AIM: To investigate the allele and genotype frequencies and associated risk of interleukin (IL )-8 -251T>A polymorphism on colorectal cancer (CRC) susceptibility risk. METHODS: Peripheral blood samples of 255 normal controls and 255 clinically and histopathologically confirmed CRC patients were genotyped for IL-8 -251T>A polymorphism employing allele-specific polymerase chain reaction. The relative association of variant allele and genotypes with CRC susceptibility risk was determined by calculating the odds ratios (ORs). Corresponding χ 2 tests on the CRC patients and controls were carried out and 95% confidence intervals (CIs) were determined using Fisher’s exact test. The allele frequencies and its risk association were calculated using FAMHAP, haplotype association analysis software. RESULTS: On comparing the frequencies of genotypes of patients and controls, the homozygous variant AA was significantly higher in CRC patients (P = 0.002) compared to controls. Investigation on the association of the polymorphic genotypes with CRC susceptibility risk, showed that the homozygous variant IL-8 -251AA had a significantly increased risk with OR 3.600 (95% CI: 1.550-8.481, P = 0.001). In the case of allele frequencies, variant allele A of IL-8 -251 showed a significantly increased risk of CRC predisposition with OR 1.32 (95% CI: 1.03-1.69, P = 0.003). CONCLUSION: Variant allele and genotype of IL-8 (-251 T>A) was significantly associated with CRC susceptibility risk and could be considered as a high-risk variant for CRC predisposition.
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spelling unimas-7342015-09-10T07:34:08Z http://ir.unimas.my/id/eprint/734/ Risk modification of colorectal cancer susceptibility by interleukin-8 -251T>A polymorphism in Malaysians Mohd Aminudin, Mustapha Siti Nurfatimah, Mohd Shahpudin Ahmad Aizat, Abdul Aziz Ankathil, Ravindran Q Science (General) R Medicine (General) AIM: To investigate the allele and genotype frequencies and associated risk of interleukin (IL )-8 -251T>A polymorphism on colorectal cancer (CRC) susceptibility risk. METHODS: Peripheral blood samples of 255 normal controls and 255 clinically and histopathologically confirmed CRC patients were genotyped for IL-8 -251T>A polymorphism employing allele-specific polymerase chain reaction. The relative association of variant allele and genotypes with CRC susceptibility risk was determined by calculating the odds ratios (ORs). Corresponding χ 2 tests on the CRC patients and controls were carried out and 95% confidence intervals (CIs) were determined using Fisher’s exact test. The allele frequencies and its risk association were calculated using FAMHAP, haplotype association analysis software. RESULTS: On comparing the frequencies of genotypes of patients and controls, the homozygous variant AA was significantly higher in CRC patients (P = 0.002) compared to controls. Investigation on the association of the polymorphic genotypes with CRC susceptibility risk, showed that the homozygous variant IL-8 -251AA had a significantly increased risk with OR 3.600 (95% CI: 1.550-8.481, P = 0.001). In the case of allele frequencies, variant allele A of IL-8 -251 showed a significantly increased risk of CRC predisposition with OR 1.32 (95% CI: 1.03-1.69, P = 0.003). CONCLUSION: Variant allele and genotype of IL-8 (-251 T>A) was significantly associated with CRC susceptibility risk and could be considered as a high-risk variant for CRC predisposition. Baishideng 2012-06-07 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/734/1/Risk%20modification%20of%20colorectal%20cancer%28abstract%29.pdf Mohd Aminudin, Mustapha and Siti Nurfatimah, Mohd Shahpudin and Ahmad Aizat, Abdul Aziz and Ankathil, Ravindran (2012) Risk modification of colorectal cancer susceptibility by interleukin-8 -251T>A polymorphism in Malaysians. World Journal of Gastroenterology., 18 (21). pp. 2668-2673. ISSN 2219 - 2840 http://www.wjgnet.com/1007-9327/full/v18/i21/2668.htm doi:10.3748/wjg.v18.i21.2668
spellingShingle Q Science (General)
R Medicine (General)
Mohd Aminudin, Mustapha
Siti Nurfatimah, Mohd Shahpudin
Ahmad Aizat, Abdul Aziz
Ankathil, Ravindran
Risk modification of colorectal cancer susceptibility by interleukin-8 -251T>A polymorphism in Malaysians
title Risk modification of colorectal cancer susceptibility by interleukin-8 -251T>A polymorphism in Malaysians
title_full Risk modification of colorectal cancer susceptibility by interleukin-8 -251T>A polymorphism in Malaysians
title_fullStr Risk modification of colorectal cancer susceptibility by interleukin-8 -251T>A polymorphism in Malaysians
title_full_unstemmed Risk modification of colorectal cancer susceptibility by interleukin-8 -251T>A polymorphism in Malaysians
title_short Risk modification of colorectal cancer susceptibility by interleukin-8 -251T>A polymorphism in Malaysians
title_sort risk modification of colorectal cancer susceptibility by interleukin-8 -251t>a polymorphism in malaysians
topic Q Science (General)
R Medicine (General)
url http://ir.unimas.my/id/eprint/734/
http://ir.unimas.my/id/eprint/734/
http://ir.unimas.my/id/eprint/734/
http://ir.unimas.my/id/eprint/734/1/Risk%20modification%20of%20colorectal%20cancer%28abstract%29.pdf