Risk modification of colorectal cancer susceptibility by interleukin-8 -251T>A polymorphism in Malaysians
AIM: To investigate the allele and genotype frequencies and associated risk of interleukin (IL )-8 -251T>A polymorphism on colorectal cancer (CRC) susceptibility risk. METHODS: Peripheral blood samples of 255 normal controls and 255 clinically and histopathologically confirmed CRC patients...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Baishideng
2012
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/734/ http://ir.unimas.my/id/eprint/734/1/Risk%20modification%20of%20colorectal%20cancer%28abstract%29.pdf |
| Summary: | AIM: To investigate the allele and genotype frequencies
and associated risk of interleukin (IL )-8 -251T>A
polymorphism on colorectal cancer (CRC) susceptibility
risk.
METHODS: Peripheral blood samples of 255 normal
controls and 255 clinically and histopathologically confirmed
CRC patients were genotyped for IL-8 -251T>A
polymorphism employing allele-specific polymerase chain
reaction. The relative association of variant allele and
genotypes with CRC susceptibility risk was determined
by calculating the odds ratios (ORs). Corresponding χ
2
tests on the CRC patients and controls were carried out
and 95% confidence intervals (CIs) were determined
using Fisher’s exact test. The allele frequencies and its
risk association were calculated using FAMHAP, haplotype
association analysis software.
RESULTS: On comparing the frequencies of genotypes
of patients and controls, the homozygous variant AA
was significantly higher in CRC patients (P = 0.002)
compared to controls. Investigation on the association
of the polymorphic genotypes with CRC susceptibility
risk, showed that the homozygous variant IL-8 -251AA
had a significantly increased risk with OR 3.600 (95%
CI: 1.550-8.481, P = 0.001). In the case of allele frequencies,
variant allele A of IL-8 -251 showed a significantly
increased risk of CRC predisposition with OR 1.32
(95% CI: 1.03-1.69, P = 0.003).
CONCLUSION: Variant allele and genotype of IL-8 (-251
T>A) was significantly associated with CRC susceptibility
risk and could be considered as a high-risk variant
for CRC predisposition. |
|---|