Optimization and validation of RP-HPLC-UV/Vis method for determination phenolic compounds in several personal care products
An HPLC method with ultraviolet-visible spectrophotometry detection has been optimized and validated for the simultaneous determination of phenolic compounds, such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) as antioxidants, and octyl methyl cinnamate (OMC) as UVB-filter in...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2011
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| Online Access: | http://ir.unimas.my/id/eprint/7329/ http://ir.unimas.my/id/eprint/7329/1/Optimization%20and%20Validation.pdf |
| _version_ | 1848836105191817216 |
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| author | Fasihuddin Badruddin, Ahmad Mohammed, Akkbik Zaini Bin, Assim |
| author_facet | Fasihuddin Badruddin, Ahmad Mohammed, Akkbik Zaini Bin, Assim |
| author_sort | Fasihuddin Badruddin, Ahmad |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | An HPLC method with ultraviolet-visible spectrophotometry detection has been optimized and validated for the simultaneous determination of phenolic compounds, such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) as antioxidants, and octyl methyl cinnamate (OMC) as UVB-filter in several personal care products. The dynamic range was between 1 to 250 mg/L with relative standard deviation less than 0.25% (n = 4). Limits of detection for BHA, BHT, and OMC were 0.196, 0.170, and 0.478 mg/L, respectively. While limits of quantification for BHA, BHT, and OMC were 0.593, 0.515, and 1.448 mg/L, respectively. The recovery for BHA, BHT, and OMC was ranged from 92.1–105.9%, 83.2–108.9%, and 87.3–103.7%, respectively. The concentration ranges of BHA, BHT, and OMC in 12 commercial personal care samples were 0.13–4.85, 0.16–2.30, and 0.12–65.5mg/g, respectively. The concentrations of phenolic compounds in these personal care samples were below than maximum allowable concentration in personal care formulation, that is, 0.0004–10 mg/g, 0.002–5mg/g, and up to 100 mg/g for BHA, BHT, and OMC, respectively. |
| first_indexed | 2025-11-15T06:18:28Z |
| format | Article |
| id | unimas-7329 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:18:28Z |
| publishDate | 2011 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-73292022-03-22T00:51:46Z http://ir.unimas.my/id/eprint/7329/ Optimization and validation of RP-HPLC-UV/Vis method for determination phenolic compounds in several personal care products Fasihuddin Badruddin, Ahmad Mohammed, Akkbik Zaini Bin, Assim QD Chemistry An HPLC method with ultraviolet-visible spectrophotometry detection has been optimized and validated for the simultaneous determination of phenolic compounds, such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) as antioxidants, and octyl methyl cinnamate (OMC) as UVB-filter in several personal care products. The dynamic range was between 1 to 250 mg/L with relative standard deviation less than 0.25% (n = 4). Limits of detection for BHA, BHT, and OMC were 0.196, 0.170, and 0.478 mg/L, respectively. While limits of quantification for BHA, BHT, and OMC were 0.593, 0.515, and 1.448 mg/L, respectively. The recovery for BHA, BHT, and OMC was ranged from 92.1–105.9%, 83.2–108.9%, and 87.3–103.7%, respectively. The concentration ranges of BHA, BHT, and OMC in 12 commercial personal care samples were 0.13–4.85, 0.16–2.30, and 0.12–65.5mg/g, respectively. The concentrations of phenolic compounds in these personal care samples were below than maximum allowable concentration in personal care formulation, that is, 0.0004–10 mg/g, 0.002–5mg/g, and up to 100 mg/g for BHA, BHT, and OMC, respectively. Hindawi Publishing Corporation 2011 Article PeerReviewed text en http://ir.unimas.my/id/eprint/7329/1/Optimization%20and%20Validation.pdf Fasihuddin Badruddin, Ahmad and Mohammed, Akkbik and Zaini Bin, Assim (2011) Optimization and validation of RP-HPLC-UV/Vis method for determination phenolic compounds in several personal care products. International journal of analytical chemistry, 2011. pp. 1-10. ISSN 1687-8760 (Print), 1687-8779 (Online) http://www.hindawi.com/journals/ijac/2011/858153/ doi:10.1155/2011/858153 |
| spellingShingle | QD Chemistry Fasihuddin Badruddin, Ahmad Mohammed, Akkbik Zaini Bin, Assim Optimization and validation of RP-HPLC-UV/Vis method for determination phenolic compounds in several personal care products |
| title | Optimization and validation of RP-HPLC-UV/Vis method for determination phenolic compounds in several personal care products |
| title_full | Optimization and validation of RP-HPLC-UV/Vis method for determination phenolic compounds in several personal care products |
| title_fullStr | Optimization and validation of RP-HPLC-UV/Vis method for determination phenolic compounds in several personal care products |
| title_full_unstemmed | Optimization and validation of RP-HPLC-UV/Vis method for determination phenolic compounds in several personal care products |
| title_short | Optimization and validation of RP-HPLC-UV/Vis method for determination phenolic compounds in several personal care products |
| title_sort | optimization and validation of rp-hplc-uv/vis method for determination phenolic compounds in several personal care products |
| topic | QD Chemistry |
| url | http://ir.unimas.my/id/eprint/7329/ http://ir.unimas.my/id/eprint/7329/ http://ir.unimas.my/id/eprint/7329/ http://ir.unimas.my/id/eprint/7329/1/Optimization%20and%20Validation.pdf |