Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia)

Bacterial resistance to various antimicrobial agents is common in area with high usage of antibiotics. In this study, the data on antimicrobial susceptibility patterns of Vibrio cholerae O1 from patients during an outbreak period was found to be high but variable rates of multidrug resistance. Thirt...

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Main Authors: Son, Radu, Micky, Vincent, Kasing, Apun, Raha, Abdul Rahim, Patrick Guda, Benjamin, Gulam, Rusul
Format: Article
Published: Elsevier 2002
Subjects:
Online Access:http://ir.unimas.my/id/eprint/7311/
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author Son, Radu
Micky, Vincent
Kasing, Apun
Raha, Abdul Rahim
Patrick Guda, Benjamin
Gulam, Rusul
author_facet Son, Radu
Micky, Vincent
Kasing, Apun
Raha, Abdul Rahim
Patrick Guda, Benjamin
Gulam, Rusul
author_sort Son, Radu
building UNIMAS Institutional Repository
collection Online Access
description Bacterial resistance to various antimicrobial agents is common in area with high usage of antibiotics. In this study, the data on antimicrobial susceptibility patterns of Vibrio cholerae O1 from patients during an outbreak period was found to be high but variable rates of multidrug resistance. Thirty-two of 33 V. cholerae isolates harboured the tcp, ctx, zot and ace genes, suggesting their possible roles in the outbreak cases. We analyzed the molecular diversity of a total of 33 strains of V. cholerae O1 isolated from 33 patients between November 1997 and April 1998 using random amplified polymorphic DNA (RAPD) analysis. The 30 typable isolates could be separated into four major clusters containing 5, 17, 2 and 6 isolates, respectively. However, no particular RAPD pattern was predictive of a particular pattern of antibiotic susceptibility. The findings of this study showed that multiple clones seemed to be responsible for cases in the outbreaks in the study area. Cited by 29
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spelling unimas-73112015-05-05T08:16:18Z http://ir.unimas.my/id/eprint/7311/ Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia) Son, Radu Micky, Vincent Kasing, Apun Raha, Abdul Rahim Patrick Guda, Benjamin Gulam, Rusul GE Environmental Sciences QR Microbiology QR355 Virology Bacterial resistance to various antimicrobial agents is common in area with high usage of antibiotics. In this study, the data on antimicrobial susceptibility patterns of Vibrio cholerae O1 from patients during an outbreak period was found to be high but variable rates of multidrug resistance. Thirty-two of 33 V. cholerae isolates harboured the tcp, ctx, zot and ace genes, suggesting their possible roles in the outbreak cases. We analyzed the molecular diversity of a total of 33 strains of V. cholerae O1 isolated from 33 patients between November 1997 and April 1998 using random amplified polymorphic DNA (RAPD) analysis. The 30 typable isolates could be separated into four major clusters containing 5, 17, 2 and 6 isolates, respectively. However, no particular RAPD pattern was predictive of a particular pattern of antibiotic susceptibility. The findings of this study showed that multiple clones seemed to be responsible for cases in the outbreaks in the study area. Cited by 29 Elsevier 2002 Article PeerReviewed Son, Radu and Micky, Vincent and Kasing, Apun and Raha, Abdul Rahim and Patrick Guda, Benjamin and Gulam, Rusul (2002) Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia). Acta Tropica, 83 (2). pp. 169-176. ISSN 0001-706X. http://qm7cd5ap7l.scholar.serialssolutions.com/?sid=google&auinit=S&aulast=Radu&atitle=Molecular+characterization+of+Vibrio+cholerae+O1+outbreak+strains+in+Miri,+Sarawak+(Malaysia)&id=doi:10.1016/S0001-706X(02)00110-9&title=Acta+tropica&volume=83&issue=2& 10.1016/S0001-706X(02)00110-9
spellingShingle GE Environmental Sciences
QR Microbiology
QR355 Virology
Son, Radu
Micky, Vincent
Kasing, Apun
Raha, Abdul Rahim
Patrick Guda, Benjamin
Gulam, Rusul
Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia)
title Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia)
title_full Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia)
title_fullStr Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia)
title_full_unstemmed Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia)
title_short Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia)
title_sort molecular characterization of vibrio cholerae o1 outbreak strains in miri, sarawak (malaysia)
topic GE Environmental Sciences
QR Microbiology
QR355 Virology
url http://ir.unimas.my/id/eprint/7311/
http://ir.unimas.my/id/eprint/7311/
http://ir.unimas.my/id/eprint/7311/