Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba
Neolamarckia cadamba or locally known as Kelampayan, is one of the fast growing plantation tree species that holds great prospect as a renewable bioresources for plywood, pulp and paper, and biofuel industries. Sufficient information on cellulose synthase (CesA) gene, especially the hypervariable...
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| Format: | Article |
| Language: | English |
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International Academy Publishing (IAP)
2014
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| Online Access: | http://ir.unimas.my/id/eprint/7298/ http://ir.unimas.my/id/eprint/7298/1/Molecular%20Cloning%20of%20Hypervariable.pdf |
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| author | Wei-Seng, Ho Shek-Ling, Pang Wee-Yang, C M., Sim |
| author_facet | Wei-Seng, Ho Shek-Ling, Pang Wee-Yang, C M., Sim |
| author_sort | Wei-Seng, Ho |
| building | UNIMAS Institutional Repository |
| collection | Online Access |
| description | Neolamarckia cadamba or locally known as Kelampayan, is one of the fast growing plantation tree
species that holds great prospect as a renewable bioresources for plywood, pulp and paper, and biofuel
industries. Sufficient information on cellulose synthase (CesA) gene, especially the hypervariable region II
(HVRII) component involved in wood formation of Kelampayan is imperative for future applications. This
region is thought to play a role in interaction with other unique cell-type-specific proteins involved in the
biosynthesis of cellulose. The aim of this study was to identify and clone the HVRII regions of cellulose
synthase gene from the developing xylem tissues of Kelampayan. The cDNA of cellulose synthase HVRII
regions was amplified using reverse transcription-PCR (RT-PCR) approach using degenerate primers. Three
clones, namely NcCesA1HVRII (520bp), NcCesA2HVRII (580bp) and NcCesA3HVRII (620bp) were
successfully sequenced and characterized. NcCesA1HVRII and NcCesA3HVRII were clustered into two
distinct clades implicated with secondary cell wall development whereas NcCesA2HVRII has renamed to
NcCslD1HVRII due its high similarity with various plants’ CslD-HVRII. This study provides an easier and
faster access to NcCesAHVRII sequences to further understand the role of NcCesA/NcCslD protein for future
applications such as selecting trees with optimal cellulose content required for specific industries. |
| first_indexed | 2025-11-15T06:18:23Z |
| format | Article |
| id | unimas-7298 |
| institution | Universiti Malaysia Sarawak |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T06:18:23Z |
| publishDate | 2014 |
| publisher | International Academy Publishing (IAP) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | unimas-72982015-09-14T03:12:38Z http://ir.unimas.my/id/eprint/7298/ Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba Wei-Seng, Ho Shek-Ling, Pang Wee-Yang, C M., Sim Q Science (General) QD Chemistry SD Forestry Neolamarckia cadamba or locally known as Kelampayan, is one of the fast growing plantation tree species that holds great prospect as a renewable bioresources for plywood, pulp and paper, and biofuel industries. Sufficient information on cellulose synthase (CesA) gene, especially the hypervariable region II (HVRII) component involved in wood formation of Kelampayan is imperative for future applications. This region is thought to play a role in interaction with other unique cell-type-specific proteins involved in the biosynthesis of cellulose. The aim of this study was to identify and clone the HVRII regions of cellulose synthase gene from the developing xylem tissues of Kelampayan. The cDNA of cellulose synthase HVRII regions was amplified using reverse transcription-PCR (RT-PCR) approach using degenerate primers. Three clones, namely NcCesA1HVRII (520bp), NcCesA2HVRII (580bp) and NcCesA3HVRII (620bp) were successfully sequenced and characterized. NcCesA1HVRII and NcCesA3HVRII were clustered into two distinct clades implicated with secondary cell wall development whereas NcCesA2HVRII has renamed to NcCslD1HVRII due its high similarity with various plants’ CslD-HVRII. This study provides an easier and faster access to NcCesAHVRII sequences to further understand the role of NcCesA/NcCslD protein for future applications such as selecting trees with optimal cellulose content required for specific industries. International Academy Publishing (IAP) 2014 Article PeerReviewed text en http://ir.unimas.my/id/eprint/7298/1/Molecular%20Cloning%20of%20Hypervariable.pdf Wei-Seng, Ho and Shek-Ling, Pang and Wee-Yang, C and M., Sim (2014) Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba. International Journal of Bioscience, Biochemistry and Bioinformatics, 4 (6). pp. 475-482. ISSN 2010-3638 http://www.ijbbb.org/show-49-727-1.html doi: 10.7763/ijbbb.2014.v4.387 |
| spellingShingle | Q Science (General) QD Chemistry SD Forestry Wei-Seng, Ho Shek-Ling, Pang Wee-Yang, C M., Sim Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba |
| title | Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba |
| title_full | Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba |
| title_fullStr | Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba |
| title_full_unstemmed | Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba |
| title_short | Molecular Cloning of Hypervariable Regions (HVRII) from Cellulose Synthase (CesA) Gene in Neolamarckia cadamba |
| title_sort | molecular cloning of hypervariable regions (hvrii) from cellulose synthase (cesa) gene in neolamarckia cadamba |
| topic | Q Science (General) QD Chemistry SD Forestry |
| url | http://ir.unimas.my/id/eprint/7298/ http://ir.unimas.my/id/eprint/7298/ http://ir.unimas.my/id/eprint/7298/ http://ir.unimas.my/id/eprint/7298/1/Molecular%20Cloning%20of%20Hypervariable.pdf |